丝状真菌Podospora anserina AA11家族裂解多糖单加氧酶基因的鉴定和功能研究

doi:10.16288/j.yczz.23-223

遗传 ›› 2023, Vol. 45 ›› Issue (12): 1128-1146.doi: 10.16288/j.yczz.23-223

丝状真菌Podospora anserina AA11家族裂解多糖单加氧酶基因的鉴定和功能研究

杜文珍¹(), 李元敬², 吴佳玲¹, 陈思羽¹, 姜亮¹, 刘刚¹, 谢宁¹()

1.深圳大学生命与海洋科学学院，深圳市微生物基因工程重点实验室，深圳 518060
2.华南师范大学材料与新能源学院，汕尾 516622

收稿日期:2023-08-21 修回日期:2023-10-28 出版日期:2023-12-20 发布日期:2023-11-10
通讯作者: 谢宁 E-mail:2100251038@email.szu.edu.cn;ning.xie@szu.edu.cn
作者简介:杜文珍，硕士研究生，专业方向：微生物遗传学。E-mail: 2100251038@email.szu.edu.cn
基金资助:
国家重点研发计划项目(2021YFA0910800);广东省自然科学基金项目(2021A1515012166);广东省自然科学基金项目(2021A1515012118);国家自然科学基金项目(31601014);国家自然科学基金项目(22078199)

Identification and functional study of AA11 family polysaccharide monooxygenase genes in filamentous fungus Podospora anserina

Wenzhen Du¹(), Yuanjing Li², Jialing Wu¹, Siyu Chen¹, Liang Jiang¹, Gang Liu¹, Ning Xie¹()

1. Shenzhen Key Laboratory of Microbial Genetic Engineering, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen 518060, China
2. School of Materials and New Energy, South China Normal University, Shanwei 516622, China

Received:2023-08-21 Revised:2023-10-28 Published:2023-12-20 Online:2023-11-10
Contact: Ning Xie E-mail:2100251038@email.szu.edu.cn;ning.xie@szu.edu.cn
Supported by:
National Key R&D Program of China(2021YFA0910800);Basic and Applied Basic Research Fund of Guangdong Province(2021A1515012166);Basic and Applied Basic Research Fund of Guangdong Province(2021A1515012118);National Natural Science Foundation of China(31601014);National Natural Science Foundation of China(22078199)

摘要/Abstract

摘要：

辅助活性蛋白家族(auxiliary activity family，AA family)中的裂解多糖单加氧酶(lytic polysaccharide monooxygenase, LPMO)能催化纤维素、几丁质和淀粉等多种难降解碳水化合物的氧化解聚。尽管目前对LPMO的酶学研究较多，但对LPMO基因失活的研究却鲜有报道。本研究利用同源重组方法定点敲除丝状真菌Podospora anserina中AA11家族的5个LPMO基因PaLPMO11A (Pa_4_4790)、PaLPMO11B (Pa_1_5310)、PaLPMO11C (Pa_2_7840)、PaLPMO11D (Pa_2_8610)和PaLPMO11E (Pa_3_9420)，分别构建了单突变体ΔPaLPMO11A (ΔA)、ΔPaLPMO11B (ΔB)、ΔPaLPMO11C (ΔC)、ΔPaLPMO11D (ΔD)和ΔPaLPMO11E (ΔE)，然后通过遗传杂交构建所有多基因突变体。通过在不同碳源培养基上的表型分析、DAB和NBT染色以及纤维素酶活测定分析野生型菌株与突变型菌株在生长速率、有性生殖、氧化应激和纤维素降解能力等方面的差异，揭示LPMO11基因在P. anserina菌株的生长发育和木质纤维素降解过程中的作用。实验结果表明，在不同纤维素碳源上，ΔBΔCΔE、ΔAΔBΔCΔE、ΔAΔCΔDΔE和ΔAΔBΔCΔDΔE突变型菌株的有性生殖能力降低，其余突变型菌株的孢子萌发效率、生长速率和生殖能力几乎没有差异。PaLPMO11家族5个基因的同时缺失，会导致菌株利用各种碳源的能力明显降低、生长速率降低、孢子萌发率降低、子实体数减少、部分子实体发育异常、寿命缩短和降解纤维素的能力显著下降，但仍有野生型45%以上的总纤维素酶活力。上述结果表明，LPMO11基因可能参与P. anserina的生长发育、有性生殖、衰老和纤维素降解过程。本研究为系统阐述丝状真菌P. anserina中木质纤维素降解的调控机制提供参考。

关键词: 丝状真菌, Podospora anserina, LPMO11, 基因敲除, 木质纤维素降解

Abstract:

The lytic polysaccharide monooxygenase (LPMO) in the auxiliary active protein family (AA family) catalyzes the oxidative depolymerization of various refractory carbohydrates including cellulose, chitin and starch. While accumulating studies investigate the enzymology of LPMO, the research on the inactivation of LPMO genes has been rarely explored. In this study, five LPMO genes PaLPMO11A (Pa_4_4790), PaLPMO11B (Pa_1_5310), PaLPMO11C (Pa_2_7840), PaLPMO11D (Pa_2_8610) and PaLPMO11E (Pa_3_9420) of the AA11 family in the filamentous fungus Podospora anserina were knocked out by homologous recombination. Single mutants ΔPaLPMO11A (ΔA), ΔPaLPMO11B (ΔB), ΔPaLPMO11C (ΔC), ΔPaLPMO11D (ΔD) and ΔPaLPMO11E (ΔE) were constructed, and then all polygenic mutants were constructed via genetic crosses. The differences in the growth rate and sexual reproduction between wild type and mutant strains were observed on different carbon source media. The alteration of oxidative stress and cellulose degradation ability were found on DAB and NBT staining and cellulase activity determination. These results implicated that LPMO11 genes play a key role in the growth, development, and lignocellulose degradation of P. anserina. The results showed that the spore germination efficiency, growth rate and reproductive capacity of mutant strains including ΔBΔCΔE, ΔAΔBΔCΔE, ΔAΔCΔDΔE and ΔAΔBΔCΔDΔE was significantly decreased on different cellulose carbon sources and the remaining strains have no difference. The reduced utilization of various carbon sources, the growth rate, the spore germination rate, the number of fruiting bodies, the normal fruiting bodies, the shortened life span and the ability to degrade cellulose were found in strains which all five genes in the PaLPMO11 family were deleted. However, the strain still had 45% cellulase activity compared to wild type. These results suggest that LPMO11 genes may be involved in the growth and development, sexual reproduction, senescence and cellulose degradation of P. anserina. This study provides information for systematically elucidating the regulatory mechanism of lignocellulose degradation in filamentous fungus P. anserina.

Key words: filamentous fungi, Podospora anserina, LPMO11, gene knockout, lignocellulose degradation

杜文珍, 李元敬, 吴佳玲, 陈思羽, 姜亮, 刘刚, 谢宁. 丝状真菌Podospora anserina AA11家族裂解多糖单加氧酶基因的鉴定和功能研究[J]. 遗传, 2023, 45(12): 1128-1146.

Wenzhen Du, Yuanjing Li, Jialing Wu, Siyu Chen, Liang Jiang, Gang Liu, Ning Xie. Identification and functional study of AA11 family polysaccharide monooxygenase genes in filamentous fungus Podospora anserina[J]. Hereditas(Beijing), 2023, 45(12): 1128-1146.

图/表 11

图1

表1

本研究使用的引物"

引物名称	序列 (5′→3′)	用途
PaLPMO11A_1F	CATTGACGCTCTCACCTTGG	敲除PaLPMO11A基因
PaLPMO11A_2R	CTATTTAACGACCCTGCCCTGAACCGATGCCCGACCTTCTGGTCCT
PaLPMO11A_MKF	AGGACCAGAAGGTCGGGCATCGGTTCAGGGCAGGGTCGTTAAATAG
PaLPMO11A_MKR	AGGTGACTACGATCCAGGCGCATCGAACTGGATCTCAACAGCGGTAAG
PaLPMO11A_3F	CTTACCGCTGTTGAGATCCAGTTCGATGCGCCTGGATCGTAGTCACCT
PaLPMO11A_4R	CCAGCCAACTTCCTCGTCAT
PaLPMO11B_1F	CCCAGACAATGACCACACGAGT	敲除PaLPMO11B基因
PaLPMO11B_2R	CTATTTAACGACCCTGCCCTGAACCGCTGCTGCATGTCACTGAAGG
PaLPMO11B_MkF	CCTTCAGTGACATGCAGCAGCGGTTCAGGGCAGGGTCGTTAAATAG
PaLPMO11B_MkR	CAAGGGTAGCTGATGAGGCACATCGAACTGGATCTCAACAGCGGTAAG
PaLPMO11B_3F	CTTACCGCTGTTGAGATCCAGTTCGATGTGCCTCATCAGCTACCCTTG
PaLPMO11B_4R	CCGCGGTGAAGCTTGACACT
PaLPMO11C_1F	ACAGGGATCATCGGTACAACTG	敲除PaLPMO11C基因
PaLPMO11C_2R	CTATTTAACGACCCTGCCCTGAACCGCTGGTTAGGTTCGAAGTCGAGT
PaLPMO11C_MKF	ACTCGACTTCGAACCTAACCAGCGGTTCAGGGCAGGGTCGTTAAATAG
PaLPMO11C_MKR	CTGGCTTCCAGTCGTCGATGCATCGAACTGGATCTCAACAGCGGTAAG
PaLPMO11C_3F	CTTACCGCTGTTGAGATCCAGTTCGATGCATCGACGACTGGAAGCCAG
PaLPMO11C_4R	CATCAGAACGACTCGCAGAT
PaLPMO11D_1F	AGAACATCCAACGTGCGTGG	敲除PaLPMO11D基因
PaLPMO11D_2R	CTATTTAACGACCCTGCCCTGAACCGAATGACAGGACAAGTGCGGT
PaLPMO11D_MKF	ACCGCACTTGTCCTGTCATTCGGTTCAGGGCAGGGTCGTTAAATAG
PaLPMO11D_MKR	CCTGACAATGCTGAAACGACTGCATCGAACTGGATCTCAACAGCGGTAAG
PaLPMO11D_3F	CTTACCGCTGTTGAGATCCAGTTCGATGCAGTCGTTTCAGCATTGTCAGG
PaLPMO11D_4R	GCATCTCCATCAGCGCAGTG
PaLPMO11E_1F	ACTAGAACGGCTCAGGCACACT	敲除PaLPMO11E基因
PaLPMO11E_2R	CTATTTAACGACCCTGCCCTGAACCGCGGATAGGCGAGTGATCGAT
PaLPMO11E_MKF	ATCGATCACTCGCCTATCCGCGGTTCAGGGCAGGGTCGTTAAATAG
PaLPMO11E_MKR	AGCTGCAGAAGTCCATCTCCCATCGAACTGGATCTCAACAGCGGTAAG
PaLPMO11E_3F	CTTACCGCTGTTGAGATCCAGTTCGATGGGAGATGGACTTCTGCAGCT
PaLPMO11E_4R	GCATGATATTCCCTCCACAGGT
5 Test	TGAGAAGCACACGGTCAC	检测目的基因的敲除
3 Test	TCGGGGCGAAAACTCTC	检测目的基因的敲除
verify_PaLPMO11A_1F	AGGCCGGATACATACCGTTG	验证PaLPMO11A基因敲除突变体
verify_PaLPMO11A_2R	CACAATGTCCTCCAACACCG	验证PaLPMO11A基因敲除突变体
verify_PaLPMO11B_1F	ACCTTGCGAGAAGGTTGGTG	验证PaLPMO11B基因敲除突变体
verify_PaLPMO11B_2R	GAGGGAGGTGGCCTCTGAT	验证PaLPMO11B基因敲除突变体
verify_PaLPMO11C_1F	GAGCAAGTCCAGCCCTGACT	验证PaLPMO11C基因敲除突变体
verify_PaLPMO11C_2R	GCAGCGCAATCGTCGTGT	验证PaLPMO11C基因敲除突变体
verify_PaLPMO11D_1F	CAGCTGAGGCTAATGATCCG	验证PaLPMO11D基因敲除突变体
verify_PaLPMO11D_2R	CTCCTCGGCAGGTCACAAGT	验证PaLPMO11D基因敲除突变体
verify_PaLPMO11E_1F	AACCAGGTTCCCCAGGATCT	验证PaLPMO11E基因敲除突变体
verify_PaLPMO11E_2R	ATAACAGGCAGCTTGGCCTT	验证PaLPMO11E基因敲除突变体

表1

图2

图3

图4

图5

图6

图7

图8

图9

图10

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丝状真菌Podospora anserina AA11家族裂解多糖单加氧酶基因的鉴定和功能研究

Identification and functional study of AA11 family polysaccharide monooxygenase genes in filamentous fungus Podospora anserina

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