CRISPR/Cas9系统在畜禽遗传改良中研究进展

doi:10.16288/j.yczz.24-021

遗传 ›› 2024, Vol. 46 ›› Issue (3): 219-231.doi: 10.16288/j.yczz.24-021

CRISPR/Cas9系统在畜禽遗传改良中研究进展

鲍艳春¹^,²(), 戴伶俐²^,³, 刘在霞¹^,², 马凤英¹^,², 王宇⁴, 刘永斌⁵, 谷明娟¹^,²(), 娜日苏¹^,²(), 张文广¹^,²^,⁶()

1.内蒙古农业大学动物科学学院，呼和浩特 010018
2.内蒙古自治区农业基因组大数据工程研究中心，呼和浩特 010018
3.内蒙古自治区农牧业科学院兽医研究所，呼和浩特 010031
4.内蒙古农业大学兽医学院，呼和浩特 010018
5.内蒙古大学生命科学学院，呼和浩特 010021
6.内蒙古农业大学生命科学学院，呼和浩特 010018

收稿日期:2024-01-17 修回日期:2024-02-25 出版日期:2024-03-20 发布日期:2024-02-29
通讯作者: 谷明娟,娜日苏,张文广 E-mail:byc107054@163.com;gmj0119@yeah.net;narisu@swu.edu.cn;actgnmbi@aliyun.com
作者简介:鲍艳春，博士研究生，专业方向：动物遗传育种与繁殖。E-mail: byc107054@163.com
基金资助:
内蒙古自治区自然科学基金项目(2021ZD05);内蒙古自治区科技计划项目(2021GG0008);内蒙古自治区科技重大专项项目(2021ZD0009);内蒙古农业大学动物科学学院高水平成果培育专项(BZX202201);内蒙古自治区直属高校基本科研业务费(BR221024)

Progress on CRISPR/Cas9 system in the genetic improvement of livestock and poultry

Yanchun Bao¹^,²(), Lingli Dai²^,³, Zaixia Liu¹^,², Fengying Ma¹^,², Yu Wang⁴, Yongbin Liu⁵, Mingjuan Gu¹^,²(), Risu Na¹^,²(), Wenguang Zhang¹^,²^,⁶()

1. College of Animal Science and Technology, Inner Mongolia Agricultural University, Hohhot 010018, China
2. Inner Mongolia Engineering Research Center of Genomic Big Data for Agriculture, Hohhot 010018, China
3. Veterinary Research Institute, Inner Mongolia Academy of Agricultural& Animal Husbandry Sciences, Hohhot 010031, China
4. College of Veterinary Medicine, Inner Mongolia Agricultural University, Hohhot 010018, China
5. College of Life Sciences, Inner Mongolia University, Hohhot 010021, China
6. College of Life Sciences, Inner Mongolia Agricultural University, Hohhot 010018, China

Received:2024-01-17 Revised:2024-02-25 Published:2024-03-20 Online:2024-02-29
Contact: Mingjuan Gu, Risu Na, Wenguang Zhang E-mail:byc107054@163.com;gmj0119@yeah.net;narisu@swu.edu.cn;actgnmbi@aliyun.com
Supported by:
Natural Science Foundation of Inner Mongolia Autonomous Region(2021ZD05);Science and Technology Plan Project of Inner Mongolia Autonomous Region(2021GG0008);Major Science and Technology Project of Inner Mongolia Autonomous Region(2021ZD0009);High Level Achievement Cultivation Project of College of Animal Science of Inner Mongolia Agricultural University(BZX202201);Basic Research Expenses of Universities Directly of Inner Mongolia Autonomous Region(BR221024)

摘要/Abstract

摘要：

CRISPR/Cas9基因编辑技术作为一种高效的基因组编辑方法，在畜牧业遗传改良领域得到了广泛的应用。该技术以高效、精准的特点，为畜牧业发展带来了一场革命。目前，基于CRISPR/Cas9的基因敲除、基因敲入和基因修饰等已被广泛应用，实现了对畜禽物种的重要生产性状进行精准改良。本文介绍了CRISPR/Cas9技术的工作原理及发展历程，重点介绍了该技术在畜禽肌肉生长发育、绒毛纤维生长、乳品质成分、抗病育种以及动物福利中的研究进展，旨在为更深入地了解CRISPR/Cas9技术在畜禽基因编辑上的应用提供参考。

关键词: CRISPR/Cas9, 基因编辑, 畜禽, 遗传改良, 经济性状

Abstract:

CRISPR/Cas9 gene editing technology, as a highly efficient genome editing method, has been extensively employed in the realm of animal husbandry for genetic improvement. With its remarkable efficiency and precision, this technology has revolutionized the field of animal husbandry. Currently, CRISPR/Cas9-based gene knockout, gene knock-in and gene modification techniques are widely employed to achieve precise enhancements in crucial production traits of livestock and poultry species. In this review, we summarize the operational principle and development history of CRISPR/Cas9 technology. Additionally, we highlight the research advancements utilizing this technology in muscle growth and development, fiber growth, milk quality composition, disease resistance breeding, and animal welfare within the livestock and poultry sectors. Our aim is to provide a more comprehensive understanding of the application of CRISPR/Cas9 technology in gene editing for livestock and poultry.

Key words: CRISPR/Cas9, gene editing, livestock and poultry, genetic improvement, economic traits

鲍艳春, 戴伶俐, 刘在霞, 马凤英, 王宇, 刘永斌, 谷明娟, 娜日苏, 张文广. CRISPR/Cas9系统在畜禽遗传改良中研究进展[J]. 遗传, 2024, 46(3): 219-231.

Yanchun Bao, Lingli Dai, Zaixia Liu, Fengying Ma, Yu Wang, Yongbin Liu, Mingjuan Gu, Risu Na, Wenguang Zhang. Progress on CRISPR/Cas9 system in the genetic improvement of livestock and poultry[J]. Hereditas(Beijing), 2024, 46(3): 219-231.

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CRISPR/Cas9系统在畜禽遗传改良中研究进展

Progress on CRISPR/Cas9 system in the genetic improvement of livestock and poultry

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