遗传 ›› 2021, Vol. 43 ›› Issue (6): 580-600.doi: 10.16288/j.yczz.21-087

• 综述 • 上一篇    下一篇

遗传修饰技术在绵羊分子设计育种中的应用

王海涛1,2(), 李亭亭3, 黄勋1,2, 马润林1,2,4(), 刘秋月1()   

  1. 1. 中国科学院种子创新研究院,北京 100101
    2. 中国科学院遗传与发育生物学研究所,分子发育生物学国家重点实验室,北京 100101
    3. 浙江农林大学,杭州 311300
    4. 中国科学院大学,北京 100049
  • 收稿日期:2021-03-08 修回日期:2021-04-14 出版日期:2021-06-20 发布日期:2021-04-30
  • 通讯作者: 马润林,刘秋月 E-mail:wanght@genetics.ac.cn;rlma@ucas.ac.cn;qyliu@genetics.ac.cn
  • 作者简介:王海涛,博士,博士后,研究方向:农业动物基因编辑。E-mail: wanght@genetics.ac.cn
  • 基金资助:
    中国科学院战略性先导科技专项(A类)资助编号(XDA24030205)

Application of genetic modification technologies in molecular design breeding of sheep

Wang Haitao1,2(), Li Tingting3, Huang Xun1,2, Ma Runlin1,2,4(), Liu Qiuyue1()   

  1. 1. Innovation Academy for Seed Design, Chinese Academy of Sciences, Beijing 100101, China
    2. State Key Laboratory of Molecular Developmental Biology, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, China
    3. Zhejiang A&F University, Hangzhou 311300, China;
    4. University of Chinese Academy of Sciences, Beijing 100049, China
  • Received:2021-03-08 Revised:2021-04-14 Online:2021-06-20 Published:2021-04-30
  • Contact: Ma Runlin,Liu Qiuyue E-mail:wanght@genetics.ac.cn;rlma@ucas.ac.cn;qyliu@genetics.ac.cn
  • Supported by:
    Supported by the Strategic Priority Research Program of Chinese Academy of Sciences No(XDA24030205)

摘要:

利用遗传修饰技术可以使动物在遗传水平发生改变,实现在个体内表达外源基因或对其内源基因的功能造成影响。在动物育种中,可以利用遗传修饰技术在分子水平进行设计并实现品种的快速改良。从传统的遗传修饰技术、病毒载体、精子载体等介导的遗传修饰技术到新型人工核酸酶介导的基因编辑技术,尤其是CRISPR/Cas9为代表的人工核酸酶的运用使得基因编辑动物的制备变得更加高效快捷,并迅速在多个物种中得到应用。这些方法也已经拓展到了绵羊(Ovis aries)遗传育种领域。利用遗传修饰技术在绵羊中进行分子育种比传统的育种方式具有更大的优势,可以使用多种策略直接对性状进行快速改良,并且可以加快育种进程。本文详细介绍了遗传修饰技术在绵羊中的研究历程,探讨了通过遗传修饰技术进行绵羊分子设计育种的可能性,并提出以上技术和方法在绵羊育种中面临的问题和挑战。

关键词: 绵羊, 遗传修饰, 基因编辑, 动物育种

Abstract:

Genetic modification technologies can be used for modifying animal genome to express exogenous genes or affect the function of endogenous genes. In animal breeding, genetic modification technologies allow the rapid generation of germplasms with beneficial traits. It includes traditional genetic modification, virus or sperm carrier-mediated genetic modification and nuclease-mediated genome editing, especially the CRISPR/Cas9, one of the artificial nuclease genome editing technologies, have been applied in genome editing in many domestic animals including sheep (Ovis aries). Compared with conventional strategies used for animal breeding, there is great value for sheep breeding improvement by using genome editing technology, which is more effective and timesaving. In this review, we summarize the approaches of genetic modification in sheep and discuss the possibility of molecular design and breeding of sheep by genome editing technologies. We also identify the potential bottlenecks and challenges of these technologies in sheep breeding.

Key words: sheep, genetic modification, genome editing, animal breeding