摘要：

对取自MAPMAKER软件小鼠F2群体(含333个体)的5个RFLP连锁标记数据作了共显性分子标记偏分离的分析。先确定选择类型的方程组(配子或合子)，随后采用Newton-Raphson迭代法估算标记间的重组值。在构建分子标记遗传图谱时，如果两个相邻标记均存在偏分离，最好采用纳入偏分离因子的估算方法。在估计F2群体标记间偏分离重组距离上,用连续χ2检测方法比传统χ2检测更为准确。Abstract The comparative analysis of segregation distortions of the codominant markers data presented in software MAPMAKER are made, where five RFLPs markers involve in a mouse F2 population with 333 individuals. The successive χ2 test begins with the determinations of gametic or zygotic selection types, followed by the estimation of recombination fractions between two markers with the Newton-Raphson iteration method. It is better to use the molecular marker showing segregation distortion for constructing a genetic map, in the case of seriously skew segregation between both the adjoining markers. The successive χ2 test provides better accuracy than that of classical χ2 test for the estimation of the recombination values in F2 population with segregation distortion.