fC31整合酶系统介导的位点特异性整合研究进展

doi:10.3724/SP.J.1005.2011.00567

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HEREDITAS ›› 2011, Vol. 33 ›› Issue (6): 567-575.doi: 10.3724/SP.J.1005.2011.00567

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Progress of fC31 integrase system in site-specific integration

MA Qing-Wen

Children’s Hospital of Shanghai, Institute of Medical Genetics, Shanghai JiaoTong University, Shanghai 200040, China

Received:2011-01-17 Revised:2011-04-06 Online:2011-06-20 Published:2011-06-25
Contact: MA Qing-Wen E-mail:maqingwen@hotmail.com

Abstract

Abstract: Integrase of phage fC31 catalyses the homologous recombination between Streptomyces attachment site attB and the phage attachment site attP. Meanwhile, this integrase can mediate integration of attB-containing donor plasmids into the pseudo attP sites in eukaryotic genomes by a site-specific manner and resulting long-term and robust expression of integrated genes. Nowadays, fC31 integrase system is becoming a potential tool for genome modification, gene therapy and transgenic research. Recent progress of fC31 integrase system in integration mode in mammalian genomes, efficiency improvement and researches concerned on transgenic safety were summarized in this review.

Key words: site-specific integration, gene therapy, transgenic, safety, fC31 integrase

MA Qing-Wen. Progress of fC31 integrase system in site-specific integration[J]. HEREDITAS, 2011, 33(6): 567-575.

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Progress of fC31 integrase system in site-specific integration

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