表观遗传学新标记--5-羟甲基胞嘧啶检测方法的研究进展

doi:10.16288/j.yczz.15-480

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HEREDITAS(Beijing) ›› 2016, Vol. 38 ›› Issue (3): 206-216.doi: 10.16288/j.yczz.15-480

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Advances on the profiling of 5-hydroxymethylcytosine

Ke Fang, Kaixiang Zhang, Jian Wang, Zhimeng Fu, Xianghui Zhao

Department of Neurobiology and Collaborative Innovation Center for Brain Science, School of Basic Medicine, Fourth Military Medical University, Xi'an 710032, China

Received:2015-11-26 Revised:2015-12-25 Online:2016-03-20 Published:2016-03-20
Supported by:
Supported by the National Natural Science Foundation of China (Nos; 31371077,31571050) and Shaanxi Science and Technology Research and Development Program (No; 2014KJXX-57)

Abstract

Abstract: 5-hydroxymethylcytosine (5hmC) is a naturally existing component in mammalian genomic DNA and is regarded as the sixth DNA base. Accumulating studies have revealed the essential role of 5hmC in embryonic development, brain function and cancer research. Compared to another well-known cytosine methylation derivate, 5-methylcytosine (5mC), the detection of 5hmC is difficult for its lower lever existing in most tissues. To distinguish 5hmC from other cytosine derivates, the methods using chemical or enzymatic DNA treatment, have been applied in targeted 5hmC detection or non-targeted 5hmC enrichment. Therefore, profiling DNA hydroxymethylcytosine by sensitive, accurate and reliable method is crucial for epigenetic study. This review discusses the principles behind recently developed techniques for 5hmC quantification and mapping. By comparing the advantages and shortcomings of these methods, the general guidelines were provided on how to select appropriate methods for specific experimental contexts.

Key words: epigenetic, hydroxymethylcytosine, profiling, high-throughput sequencing

Ke Fang, Kaixiang Zhang, Jian Wang, Zhimeng Fu, Xianghui Zhao. Advances on the profiling of 5-hydroxymethylcytosine[J]. HEREDITAS(Beijing), 2016, 38(3): 206-216.

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