关键词: 单倍型, 汉族人群, 基因型, 细胞因子

Abstract:

In order to investigate the genetic polymorphism of 13 cytokines genes in Han individuals from Zhejiang. The polymerase chain reaction-sequence specific primers (PCR-SSP) method was used to determine genetic polymorphisms in 100 unrelated healthy Han individuals from Zhejiang province. We found that: (1)The frequency of the TGFb (G codon 25) allele is 1.00 and no TGFb (C codon 25) allele is found. (2) The frequencies of IL-1a (C-889), IL-1b(C +3962), IL-4 (T-1098), IL-6 (G-174), IL-6 (G nt565), IL-10 (A-1082) and TNFa (G-238) alleles are more than 0.95; while the frequen-cies of IL-1a (T-889), IL-1a (T +3962), IL-4 (G-1098), IL-6 (A-174), IL-6 (A nt565), IL-10 (G-1082), TNFa (A-238) al-leles are less than 0.05. (3) The haplotype frequency of the high-producer-type GCC in IL-10 gene is 0.05, while the low-producer-type ATA is 0.735. The haplotype frequency of the high-producer-type TG in the TGFb gene is 0.49, while the low-producer-type CC is 0. The hap lotype frequency of the high-producer-types AG and AA in the TNFa gene is 0.055, while of the low-producer-types GG and GA is 0.945. The results showed that the genetic polymorphism of cytokine genes in Han individuals from Zhejiang Province is distinctive.