遗传 ›› 2007, Vol. 29 ›› Issue (9): 1077-1082.doi: 10.1360/yc-007-1077

• 研究报告 • 上一篇    下一篇

山羊MyoD基因家族多态性及与体尺性状的相关性

张海军1, 3, 陈宏1, 2, 房兴堂1, 张润锋2, 鲍斌1, 高雪原1, 邵汝英1   

  1. 1. 徐州师范大学细胞与分子生物学研究所, 州 221116; 2. 西北农林科技大学动物科技学院, 陕西省农业分子生物学重点实验室, 陕西杨凌 712100; 3. 江苏省海门中学, 江苏海门 226100

  • 收稿日期:2007-02-28 修回日期:2007-05-15 出版日期:2007-09-10 发布日期:2007-09-10
  • 通讯作者: 陈宏

Association of variations in MyoD family of genes with body meas-urement traits in goat

ZHANG Hai-Jun1,3, CHEN Hong1,2, FANG Xing-Tang1, ZHANG RunFeng2, BAO Bin1, GAO Xue-Yuan1, SHAO Ru-Ying1
  

  1. 1. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China; 2. College of Animal Science and Technology, Northwest A&F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China; 3. Haimen Middle School of Jiangsu Province, Haimen, Jiangsu 226100, China
  • Received:2007-02-28 Revised:2007-05-15 Online:2007-09-10 Published:2007-09-10
  • Contact: CHEN Hong

摘要:

用PCR-SSCP技术研究了波尔山羊和徐淮山羊2个群体共147个个体MyoD基因家族中3个基因座位的单核苷酸多态性(single nucleotide polymorphism, SNP)。结果表明, 在徐淮白山羊群体中, myf-5基因座发现有3种基因型AA、ABBB, 波尔山羊均为AA型。在myf-6基因座和myoD 5′侧翼区基因座, 两个山羊群体均检测到了AAAB型个体。对山羊myf-5、myf-6基因座, myoD 5′侧翼区基因座不同基因型与两品种山羊体尺性状相关分析表明, myf-5基因座对管围和管围指数的效应差异显著(P<0.05)。myf-6基因座对徐淮白山羊体尺性状的效应均不显著(P>0.05), 而对波尔山羊的体高和管围指数效应差异显著(P<0.05)。两个山羊品种myoD 5′侧翼区不同基因型个体的体尺性状差异均不显著。

关键词: myf-6, MyoD, 遗传多态性, myf-5, 山羊

Abstract:

Single nucleotide polymorphisms (SNPs) of hircine myf-5, myf-6 genes and 5′flanking region of the myoD gene (myoD 5′) were analyzed by PCR-SSCP in Boer goat and Xuhuai white goat. Three genotypes were found at the myf-5 locus of Xuhuai white goat, denoted AA, AB and BB, while all of Boer goats were of AA genotype. Only AA and AB genotypes were found at myf-6 and myoD 5′ loci. The effects of genotypes of myf-5, myf-6 and myoD 5′flanking region loci on body measurement traits were estimated. The results showed that genotypes at the myf-5 locus were significantly associated with hucklebone width and hucklebone width index in Xuhuai white goat (P<0.05). Genotypes at the myf-6 locus were significantly associated with withers heights and hucklebone width index in Boer goat (P<0.05), but no significant associations were found for any traits in Xuhuai white goat. The myoD 5′ locus was not significantly associated with any traits in both types of goat.