遗传 ›› 2016, Vol. 38 ›› Issue (3): 227-242.doi: 10.16288/j.yczz.15-327
周想春1, 邢永忠1, 2
收稿日期:2015-07-20
修回日期:2016-01-04
出版日期:2016-03-20
发布日期:2016-03-20
通讯作者:
邢永忠,教授,博士生导师,研究方向:生物化学与分子生物学.
E-mail:yzxing@mail.hzau.edu.cn
作者简介:周想春,博士研究生,研究方向:遗传学.E-mail: xiangchunzhou@sina.com
基金资助:Xiangchun Zhou1, Yongzhong Xing1, 2
Received:2015-07-20
Revised:2016-01-04
Online:2016-03-20
Published:2016-03-20
Supported by:摘要： 利用生物技术可以对植物基因组进行高效,精准,特异的修饰.锌指核酸酶(Zinc finger nucleases, ZFN),转录激活样效应因子核酸酶(Transcription activator-like effector nucleases, TALEN),成簇规律间隔短回文重复序列(Clustered regularly interspaced short palindromic repeats, CRISPR)/Cas9 (CRISPR-associated 9)是目前基因组编辑技术应用中的关键工程核酸酶.通过产生DNA双链断裂(Double-strand breaks, DSBs)激活植物内源修复途径(包括非同源粘性末端连接和同源重组修复),基因组编辑技术可以实现对靶位点的定点突变,缺失或者基因的插入与替换.基因组编辑已经被广泛地应用到各种植物的基因组修饰中,如拟南芥,水稻,烟草等.本文主要概述了基因组编辑技术在植物基因功能鉴定及作物遗传育种中的应用,并对其未来在作物精准改良中需要完善的相关问题进行了探讨.
周想春, 邢永忠. 基因组编辑技术在植物基因功能鉴定及作物育种中的应用[J]. 遗传, 2016, 38(3): 227-242.
Xiangchun Zhou, Yongzhong Xing. The application of genome editing in identification of plant gene function and crop breeding[J]. HEREDITAS(Beijing), 2016, 38(3): 227-242.
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