欧洲千里光SvAPETALA1-5基因对花器官发育的影响

doi:10.16288/j.yczz.24-147

遗传 ›› 2024, Vol. 46 ›› Issue (9): 727-736.doi: 10.16288/j.yczz.24-147

欧洲千里光SvAPETALA1-5基因对花器官发育的影响

张玉娜(), 郝燕敏, 崔敏龙, 朴春兰()

浙江农林大学园艺科学学院，浙江省山区农业高效绿色生产协同创新中心，农业农村部亚热带果品蔬菜质量安全控制重点实验室，杭州 311300

收稿日期:2024-05-23 修回日期:2024-07-17 出版日期:2024-09-20 发布日期:2024-08-01
通讯作者: 朴春兰，硕士，助理研究员，研究方向：生物技术与分子育种。E-mail: 2488335116@qq.com
作者简介:张玉娜，硕士研究生，专业方向：植物花器官发育。E-mail: 1047925191@qq.com
基金资助:
浙江省农业科学院合作研发项目(H20220312)

Effects of SvAPETALA1-5 gene on floral organ development in Senecio vulgaris

Yuna Zhang(), Yanmin Hao, Minlong Cui, Chunlan Piao()

Key Laboratory of Quality and Safety Control for Subtropical Fruit and Vegetable,Ministry of Agriculture and Rural Affairs, Collaborative Innovation Center for Efficient and Green Production of Agriculture in Mountainous Areas of Zhejiang Province, College of Horticultural Science, Zhejiang A & F University, Hangzhou 311300, China

Received:2024-05-23 Revised:2024-07-17 Published:2024-09-20 Online:2024-08-01
Supported by:
Cooperative R&D Project of Zhejiang Academy of Agricultural Sciences(H20220312)

摘要/Abstract

摘要：

菊科(Asteraceae)是真双子叶植物的一大类，具有复杂的头状花序，目前对其花发育的分子调控机制还知之甚少。APETALA1(AP1)属于MADS-box基因家族，在植物成花诱导和花器官发育中具有关键作用。本研究对欧洲千里光(Senecio vulgaris)AP1同源基因SvAP1-5进行了生物信息学及组织特异性表达分析，并基于VIGS技术创建了SvAP1-5基因沉默植株，并在龙葵(Solanum nigrum)中过表达SvAP1-5，对其功能进行了初步探讨。生物信息学分析结果表明，SvAP1-5基因具有典型的MADS-box和K-box结构，C末端含有FUL基序和paleoAP1基序，属于euFUL分支的AP1类基因。qRT-PCR结果表明，SvAP1-5在苞片、花瓣和心皮中均有表达，其中在心皮内较高表达。与对照相比，SvAP1-5基因沉默导致花瓣不规则裂开，柱头分裂增加，心皮发育不良。而SvAP1-5过表达龙葵植株的果实发育异常，出现类似果实细胞的增生组织。综上所述，在欧洲千里光花发育过程中，SvAP1-5基因可能参与花瓣和心皮的发育，并发挥着重要作用。

关键词: 欧洲千里光, SvAPETALA1-5, 花发育, 功能研究

Abstract:

Asteraceae is a large class of eudicots with complex capitulum, and little is known regarding the molecular regulation mechanism of flower development. APETALA1(AP1) belongs to the MADS-box gene family and plays a key role in plant floral induction and floral organ development. In this study, the bioinformatics and tissue-specific expression of AP1 homologous gene SvAP1-5 in Senecio vulgaris were analyzed. Based on VIGS technology, SvAP1-5 gene silencing plants were created, and SvAP1-5 was overexpressed in Solanum nigrum. The results of bioinformatics analysis showed that SvAP1-5 gene had typical MADS-box and K-box structure, and contains FUL motif and paleoAP1 motif at the C-terminal. SvAP1-5 belongs to the euFUL branch of AP1 gene. qRT-PCR results showed that SvAP1-5 was expressed in bracts, petals and carpels, and was highly expressed in carpels. Compared with the control group, SvAP1-5 gene silencing resulted in irregular petal dehiscence, increased stigma division, and carpel dysplasia. The fruit development of SvAP1-5 overexpressing S.nigrum plants was abnormal, and the hyperplastic tissue similar to fruit appeared. In summary, SvAP1-5 gene may be involved in the development of petals and carpels and plays an important role during the development of S.vulgaris.

Key words: Senecio vulgaris, SvAPETALA1-5, flower development, functional research

张玉娜, 郝燕敏, 崔敏龙, 朴春兰. 欧洲千里光SvAPETALA1-5基因对花器官发育的影响[J]. 遗传, 2024, 46(9): 727-736.

Yuna Zhang, Yanmin Hao, Minlong Cui, Chunlan Piao. Effects of SvAPETALA1-5 gene on floral organ development in Senecio vulgaris[J]. Hereditas(Beijing), 2024, 46(9): 727-736.

图/表 7

表1

图1

图2

图3

图4

图5

图6

参考文献 33

[1]	Fornara F, de Montaigu A, Coupland G. SnapShot: control of flowering in Arabidopsis. Cell, 2010, 141(3): 550. e1-550.e2.
[2]	Smaczniak C, Immink RGH, Angenent GC, Kaufmann K. Developmental and evolutionary diversity of plant MADS-domain factors: insights from recent studies. Development, 2012, 139(17): 3081-3098. doi: 10.1242/dev.074674 pmid: 22872082
[3]	Mandel MA, Gustafson-Brown C, Savidge B, Yanofsky MF. Molecular characterization of the Arabidopsis floral homeotic gene APETALA1. Nature, 1992, 360(6401): 273-277.
[4]	Müller BM, Saedler H, Zachgo S. The MADS-box gene DEFH28 from Antirrhinum is involved in the regulation of floral meristem identity and fruit development. Plant J, 2001, 28(2): 169-179. pmid: 11722760
[5]	Coen ES, Meyerowitz EM. The war of the whorls: genetic interactions controlling flower development. Nature, 1991, 353(6339): 31-37.
[6]	Gustafson-Brown C, Savidge B, Yanofsky MF. Regulation of the Arabidopsis floral homeotic gene APETALA1. Cell, 1994, 76(1): 131-143.
[7]	Zhang CL, Sun YL, Yu XM, Li H, Bao MZ, He YH. Functional conservation and divergence of five AP1/FUL-like genes in Marigold (Tagetes erecta L.). Genes (Basel), 2021, 12(12): 2011.
[8]	Monniaux M, Pieper B, McKim SM, Routier- Kierzkowska AL, Kierzkowski D, Smith RS, Hay A. The role of APETALA1 in petal number robustness. Elife, 2018, 7: e39399.
[9]	Sawettalake N, Bunnag S, Wang YW, Shen LS, Yu H. DOAP1 promotes flowering in the orchid Dendrobium Chao Praya Smile. Front Plant Sci, 2017, 8: 400. doi: 10.3389/fpls.2017.00400 pmid: 28386268
[10]	Zhang SS, Lu SJ, Yi SS, Han HJ, Zhou Q, Cai FF, Bao MZ, Liu GF. Identification and characterization of FRUITFULL-like genes from Platanus acerifolia, a basal eudicot tree. Plant Sci, 2019, 280: 206-218. doi: S0168-9452(18)30749-0 pmid: 30823999
[11]	Lai XL, Vega-Léon R, Hugouvieux V, Blanc-Mathieu R, van der Wal F, Lucas J, Silva CS, Jourdain A, Muino JM, Nanao MH, Immink R, Kaufmann K, Parcy F, Smaczniak C, Zubieta C. The intervening domain is required for DNA-binding and functional identity of plant MADS transcription factors. Nat Commun, 2021, 12(1): 4760. doi: 10.1038/s41467-021-24978-w pmid: 34362909
[12]	Yue YL, Sun S, Li JW, Yu HD, Wu HX, Sun BQ, Li T, Han TF, Jiang BJ. GmFULa improves soybean yield by enhancing carbon assimilation without altering flowering time or maturity. Plant Cell Rep, 2021, 40(10): 1875-1888.
[13]	Jiang XB, Lubini G, Hernandes-Lopes J, Rijnsburger K, Veltkamp V, De Maagd RA, Angenent GC, Bemer M. FRUITFULL-like genes regulate flowering time and inflorescence architecture in tomato. Plant Cell, 2022, 34(3): 1002-1019.
[14]	Ruokolainen S, Ng YP, Albert VA, Elomaa P, Teeri TH. Over-expression of the Gerbera hybrida At-SOC1- like1 gene Gh-SOC1 leads to floral organ identity deterioration. Ann Bot, 2011, 107(9): 1491-1499.
[15]	Goloveshkina EN, Shul'ga OA, Shchennikova AV, Kamionskaya AM, Skryabin KG. Constitutive expression of the sunflower and chrysanthemum genes of the AP1/FUL group changes flowering timing in transgenic tobacco plants. Dokl Biol Sci, 2010, 434: 322-324.
[16]	Fambrini M, Bernardi R, Pugliesi C. Ray flower initiation in the Helianthus radula inflorescence is influenced by a functional allele of the HrCYC2c gene. Genesis, 2020, 58(12): e23401.
[17]	Luan SN, Liu LL, Zhou JY, Imam N, Cui ML, Piao CL. Functional analysis of flower development related gene SvGLOBOSA from Senecio vulgaris. Hereditas(Beijing), 2022, 44(6): 521-530.
	栾思楠, 刘乐乐, 周佳圆,努尔阿斯娅·伊马木, 崔敏龙, 朴春兰. 欧洲千里光花发育相关基因SvGLOBOSA功能研究. 遗传, 2022, 44(6): 521-530.
[18]	Li FF, Hao YM, Cui ML, Piao CL. Cloning and functional analysis of RADIALIS-like 1 gene from Antirrhinum majus. Hereditas(Beijing), 2023, 45(6): 526-535.
	李菲菲, 郝燕敏, 崔敏龙, 朴春兰. 金鱼草RADIALIS-like 1基因克隆与功能研究. 遗传, 2023, 45(6): 526-535.
[19]	Nicholas AZ. Flower head development in the asteraceae family[Dissertation]. The University of Manchester, 2014.
[20]	Hao YM, Chen KL, Feng LJ, Li FF, Cui ML, Piao CL. Cloning and functional analysis of SvAPETALA1 in Senecio vulgaris. J Zhejiang A F Univ, 2022, 39(4): 821-829.
	郝燕敏, 陈柯俐, 冯丽君, 李菲菲, 崔敏龙, 朴春兰. 欧洲千里光SvAPETALA1基因的克隆及功能分析. 浙江农林大学学报, 2022, 39(4): 821-829.
[21]	Østergaard L, Kempin SA, Bies D, Klee HJ, Yanofsky MF. Pod shatter-resistant Brassica fruit produced by ectopic expression of the FRUITFULL gene. Plant Biotechnol J, 2006, 4(1): 45-51. doi: 10.1111/j.1467-7652.2005.00156.x pmid: 17177784
[22]	Morel P, Chambrier P, Boltz V, Chamot S, Rozier F, Rodrigues Bento S, Trehin C, Monniaux M, Zethof J, Vandenbussche M. Divergent functional diversification patterns in the SEP/AGL6/AP1 MADS-Box transcription factor superclade. Plant Cell, 2019, 31(12): 3033- 3056.
[23]	Shchennikova AV, Shulga OA, Immink R, Skryabin KG, Angenent GC. Identification and characterization of four chrysanthemum MADS-box genes, belonging to the APETALA1/FRUITFULL and SEPALLATA3 subfamilies. Plant Physiol, 2004, 134(4): 1632-1641. pmid: 15064378
[24]	Sather DN, Golenberg EM. Duplication of AP1 within the Spinacia oleracea L. AP1/FUL clade is followed by rapid amino acid and regulatory evolution. Planta, 2009, 229(3): 507-521. doi: 10.1007/s00425-008-0851-9 pmid: 19005675
[25]	Burko Y, Shleizer-Burko S, Yanai O, Shwartz I, Zelnik ID, Jacob-Hirsch J, Kela I, Eshed-Williams L, Ori N. A role for APETALA1/FRUITFULL transcription factors in tomato leaf development. Plant Cell, 2013, 25(6): 2070-2083.
[26]	Zhang WX, Fan SL, Pang CY, Wei HL, Ma JH, Song MZ, Yu SX. Molecular cloning and function analysis of two SQUAMOSA-like MADS-box genes from Gossypium hirsutum L. J Integr Plant Biol, 2013, 55(7): 597-607.
[27]	Ruokolainen S, Ng YP, Broholm SK, Albert VA, Elomaa P, Teeri TH. Characterization of SQUAMOSA- like genes in Gerbera hybrida, including one involved in reproductive transition. BMC Plant Biol, 2010, 10: 128. doi: 10.1186/1471-2229-10-128 pmid: 20579337
[28]	Bemer M, Karlova R, Ballester AR, Tikunov YM, Bovy AG, Wolters-Arts M, Rossetto Pde B, Angenent GC, de Maagd RA. The tomato FRUITFULL homologs TDR4/FUL1 and MBP7/FUL2regulate ethylene- independent aspects of fruit ripening. Plant Cell, 2012, 24(11): 4437-4451.
[29]	Pabón-mora N, Ambrose BA, Litt A. Poppy APETALA1/FRUITFULL orthologs control flowering time, branching, perianth identity, and fruit development. Plant Physiol, 2012, 158(4): 1685-1704. doi: 10.1104/pp.111.192104 pmid: 22286183
[30]	Ferrándiz C, Fourquin C. Role of the FUL-SHP network in the evolution of fruit morphology and function. J Exp Bot, 2014, 65(16): 4505-4513. doi: 10.1093/jxb/ert479 pmid: 24482369
[31]	Liu CJ, Zhang J, Zhang N, Shan HY, Su KM, Zhang JS, Meng Z, Kong HZ, Chen ZD. Interactions among proteins of floral MADS-box genes in basal eudicots: implications for evolution of the regulatory network for flower development. Mol Biol Evol, 2010, 27(7): 1598-1611. doi: 10.1093/molbev/msq044 pmid: 20147438
[32]	Ma GY, Zou QC, Shi XH, Tian DQ, Sheng QQ. Ectopic expression of the AaFUL1gene identified in Anthurium andraeanum affected floral organ development and seed fertility in tobacco. Gene, 2019, 696: 197-205.
[33]	Jaakola L, Poole M, Jones MO, Kämäräinen-Karppinen T, Koskimäki JJ, Hohtola A, Häggman H, Fraser PD, Manning K, King GJ, Thomson H, Seymour GB. A SQUAMOSA MADS box gene involved in the regulation of anthocyanin accumulation in bilberry fruits. Plant Physiol, 2010, 153(4): 1619-1629.

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

引物名称	引物序列(5′→3′)	用途
TRV2::SvAP1-5	F：AACTCGAGCAATCTCTCAGCTCCAA	VIGS载体构建
TRV2::SvAP1-5	R：AAGGATCCGATGAATCATCCAAGGAGGC	VIGS载体构建
qSvAP1-5	F：GTTGTGTGATGCTGACGTGG	qRT-PCR引物
qSvAP1-5	R：GCGTGTTCCAGAGTCCAGTT	qRT-PCR引物
Sv18S	F：CGCGCGCTACACTGATGTATTCAA	内参基因
Sv18S	R：TACAAAGGGCAGGGACGTAGTCAA	内参基因

欧洲千里光SvAPETALA1-5基因对花器官发育的影响

Effects of SvAPETALA1-5 gene on floral organ development in Senecio vulgaris

RichHTML

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

图/表 7

参考文献 33

相关文章 7

编辑推荐

Metrics

[1]	李菲菲, 郝燕敏, 崔敏龙, 朴春兰. 金鱼草RADIALIS-like 1基因克隆与功能研究[J]. 遗传, 2023, 45(6): 526-535.
[2]	栾思楠, 刘乐乐, 周佳圆, 努尔阿斯娅·伊马木, 崔敏龙, 朴春兰. 欧洲千里光花发育相关基因SvGLOBOSA功能研究[J]. 遗传, 2022, 44(6): 521-530.
[3]	郭文雅,崔艳梅,王婷婷,喻德跃,黄方. 野生大豆花发育相关基因GsLFY的功能研究[J]. 遗传, 2017, 39(1): 56-65.
[4]	周坤, 张今今. 植物中的NO及其对花发育的调节[J]. 遗传, 2014, 36(7): 661-668.
[5]	张向前，邹金松，朱海涛，李晓燕，曾瑞珍. 水稻早熟多子房突变体fon5的遗传分析和基因定位[J]. 遗传, 2008, 30(10): 1349-1355.
[6]	袁晓萌，周云涛，张红岩，薛华，周琳，赵云. 甘蓝型油菜小核糖核蛋白BnSmD1编码区全长cDNA 的克隆与表达分析[J]. 遗传, 2007, 29(12): 1525-1528.
[7]	王利琳，梁海曼，庞基良，朱睦元. 拟南芥LEAFY基因在花发育中的网络调控及其生物学功能[J]. 遗传, 2004, 26(1): 137-142.