遗传 ›› 2008, Vol. 30 ›› Issue (12): 1529-1535.doi: 10.3724/SP.J.1005.2008.01529

• 综述 • 上一篇    下一篇

肿瘤染色体畸变分析方法新进展

薛渊博; 宋鑫   

  1. 昆明医学院第三附属医院(云南省肿瘤医院) 肿瘤生物治疗中心, 昆明 650018
  • 收稿日期:2008-04-29 修回日期:2008-07-23 出版日期:2008-12-10 发布日期:2008-12-10
  • 通讯作者: 宋鑫

Progresses on the methods of tumor chromosome aberration analysis

XUE Yuan-Bo; SONG Xin   

  1. Center of Cancer Biotherapy, The Third Affiliated Hospital of Kunming Medical University (Tumor Hospital of Yunnan Province),
    Kunming
    650018, China
  • Received:2008-04-29 Revised:2008-07-23 Online:2008-12-10 Published:2008-12-10
  • Contact: SONG Xin

摘要: 摘要: 肿瘤的发生多与染色体畸变有关, 确定染色体畸变与肿瘤的关系, 必然离不开染色体畸变的检测分析。文章简要综述几种常用染色体畸变的检测方法及其新进展, 包括G显带、荧光原位杂交(FISH )、光谱核型分析(SKY)、多色荧光原位杂交(M-FISH)、多色显带分析技术(Rx-FISH)、比较基因组杂交(CGH)和微阵列比较基因组杂交(Array CGH), 以及这些方法在肿瘤诊断和研究方面的应用。

关键词: 检测, 染色体杂交, 染色体分析, 染色体

Abstract: Abstract: Most cancers are known to be associated with chromosome aberration, and chromosome analysis is essential to understand the relationships between chromosome aberration and cancer. Here we briefly introduce several methods of chromosome aberration detection, including G-banding, fluorescence in situ hybridization (FISH), spectral karyotyping (SKY), multi-fluorescence in situ hybridization (M-FISH), cross-species color banding (Rx-FISH), comparative genomic hybridization (CGH)and Array comparative genomic hybridization (Array CGH).