遗传 ›› 2009, Vol. 31 ›› Issue (6): 573-580.doi: 10.3724/SP.J.1005.2009.00573

• 综述 • 上一篇    下一篇

原子力显微镜在染色体研究中的应用

李琦1;郑琪1;丁焰1;马璐2;李立家2   

  1. 1. 西北工业大学生命科学院实验中心, 西安 710072;
    2. 武汉大学生命科学院植物发育生物学教育部重点实验室, 武汉 430072
  • 收稿日期:2008-10-17 修回日期:2009-01-26 出版日期:2009-06-10 发布日期:2009-06-10
  • 通讯作者: 李立家

Application of atomic force microscopy in chromosome research

LI Qi1;ZHENG Qi1;DING Yan1;MA Lu2;LI Li-Jia2   

  1. 1. Faculty of Life Sciences, Northwestern Polytechnic University, Xi’an 710072, China;
    2. Key Laboratory for Plant Developmental Biology of the Ministry of Education, College of Life Sciences, Wuhan University, Wuhan 430072, China
  • Received:2008-10-17 Revised:2009-01-26 Online:2009-06-10 Published:2009-06-10
  • Contact: LI Lijia

摘要: 原子力显微镜(Atomic force microscopy, AFM)是一种具有超高分辨率的显微成像仪器, 可在空气、真空和液体环境下对样本的表面结构进行实时观察。文章介绍了AFM的工作原理, AFM相对于其他种类显微镜在观察生物样本方面的显著优势, 并综述了AFM在染色体研究中的应用和进展。

关键词: 原子力显微镜, 染色体, 超微结构

Abstract: Atomic force microscopy (AFM) is an effective apparatus for examination of the surface structure of specimens with a higher resolution. It can be used for real-time observation in vacuum, air, and liquid conditions. This review pre-sented the basic principle of AFM and its significant advantages in biological sample research relative to other types of microscopes and summarized its application in chromosome research.