一种栉孔扇贝丝氨酸蛋白酶基因的染色体定位及其内部SNP的研究

doi:10.3724/SP.J.1005.2009.01.004

遗传 ›› 2009, Vol. 31 ›› Issue (12): 1241-1247.doi: 10.3724/SP.J.1005.2009.01.004

一种栉孔扇贝丝氨酸蛋白酶基因的染色体定位及其内部SNP的研究

郇聘^{1, 2}, 张晓军¹, 李富花¹, 张洋^{1, 3}, 赵翠^{1, 2}, 刘保忠¹, 相建海¹

1. 中国科学院海洋研究所, 青岛 266071;
2. 中国科学院研究生院, 北京 100039;
3. Department of Soil & Crop Sciences, College Station, Texas A&M University, TX 77843-2472, USA

收稿日期:2009-03-06 修回日期:2009-10-09 出版日期:2009-12-10 发布日期:2009-11-17
通讯作者: 相建海 E-mail:jhxiang@ms.qdio.ac.cn
基金资助:
国家自然科学基金重点项目(编号：30730071)和国家高技术研究发展计划(863)项目(编号：2007AA09Z430)资助

Chromosomal localization and development of SNP markers of a serine protease gene in Farrer’s scallop (Chlamys farreri)

HUAN Pin^{1, 2}, ZHANG Xiao-Jun¹, LI Fu-Hua¹, ZHANG Yang^{1, 3}, ZHAO Cui¹, LIU Bao-Zhong¹, XIANG Jian-Hai¹

1. Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;
2. Graduate School of Chinese Academy of Sciences, Beijing 100039, China;
3. Department of Soil & Crop Sciences, College Station, Texas A & M University, TX 77843-2472, USA

Received:2009-03-06 Revised:2009-10-09 Online:2009-12-10 Published:2009-11-17
Contact: XIANG Jian-Hai E-mail:jhxiang@ms.qdio.ac.cn

摘要/Abstract

摘要：

病害问题是制约我国扇贝养殖业发展的关键, 因此贝类的先天免疫也成为当前研究的热点。丝氨酸蛋白酶是先天免疫中至关重要的酶类，在许多通路中起信号放大作用。目前对栉孔扇贝丝氨酸蛋白酶的研究主要集中于基因序列分析和表达谱研究, 基因定位方面的研究尚未开展。文章以包含一种栉孔扇贝丝氨酸蛋白酶基因的BAC克隆为探针, 利用BAC-FISH技术将其定位到一对同源染色体的长臂上, 为丝氨酸蛋白酶基因的后续研究提供了基础; 同时, 利用PCR产物直接测序法筛选了该基因内部的6个SNP标记, 这些SNP标记可供遗传图谱定位使用, 从而可以实现遗传图谱与染色体间的锚定与初步整合

关键词: 栉孔扇贝, 丝氨酸蛋白酶, 荧光原位杂交, 染色体, 单核苷酸多态性

Abstract:

Disease control is a main objective in the culture of Farrer’s scallop (Chalmys farreri). Many investigations have focused on the innate immune system of this species. Serine proteases amplify signals in many immune-related pathways and thus play a crucial role in innate immune system. Recently, studies on serine protease in C. farreri were mainly on sequence analysis and expression profile, but no study on gene localization was reported. In this study, a BAC clone (CFB040H03) that contains a serine protease gene of C. farreri was located on the long arms of a pair of homologous chromosomes by BAC-FISH technique. Six SNPs were found inside the serine protease gene using direct sequencing of the PCR products. The chromosomal localization of serine protease gene would provide fundamental support for further research on this gene. The SNPs obtained can be mapped to the genetic linkage map and is helpful to the construction of the integration map of C. farreri.

Key words: Chalmys farreri, serine protease, fluorescence in situ hybridization (FISH), chromosome, single nucleotide polymorphism (SNP)

郇聘，张晓军，李富花，张洋，赵翠，刘保忠，相建海. 一种栉孔扇贝丝氨酸蛋白酶基因的染色体定位及其内部SNP的研究[J]. 遗传, 2009, 31(12): 1241-1247.

HUAN Pin, ZHANG Xiao-Jun, LI Fu-Hua, ZHANG Xiang, DIAO Cui, LIU Bao-Zhong, XIANG Jian-Hai. Chromosomal localization and development of SNP markers of a serine protease gene in Farrer’s scallop (Chlamys farreri)[J]. HEREDITAS, 2009, 31(12): 1241-1247.

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一种栉孔扇贝丝氨酸蛋白酶基因的染色体定位及其内部SNP的研究

Chromosomal localization and development of SNP markers of a serine protease gene in Farrer’s scallop (Chlamys farreri)

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