玉米近缘种中玉米着丝粒序列的FISH检测

doi:10.3724/SP.J.1005.2010.00264

遗传 ›› 2010, Vol. 32 ›› Issue (3): 264-270.doi: 10.3724/SP.J.1005.2010.00264

玉米近缘种中玉米着丝粒序列的FISH检测

佘朝文^{1, 2},蒋向辉^{1, 2},宋运淳³,刘伟¹

1. 怀化学院生命科学系, 湖南怀化 418008;
2. 怀化学院民族药用植物资源研究与利用湖南省重点实验室, 湖南怀化 418008;
3. 武汉大学植物发育生物学教育部重点实验室, 武汉 430072

收稿日期:2009-08-10 修回日期:2009-10-20 出版日期:2010-03-20 发布日期:2010-03-15
通讯作者: 佘朝文 E-mail:shechaowen@tom.com
基金资助:
湖南省教育厅科研基金项目(编号：07C502)资助

Detection of maize centromeric repeats in the relatives of maize using fluorescence in situ hybridization

SHE Chao-Wen^{1, 2},JIANG Xiang-Hui^{1, 2},SONG Yun-Chun³, LIU Wei¹

1. Department of Life Sciences, Huaihua University, Huaihua 418008, China;
2. Key Laboratory of Study and Utilization of Ethnomedicinal Plant Resources of Hunan Province, Huaihua University, Huaihua 418008, China;
3. Key Laboratory of MOE for Plant Developmental Biology, Wuhan University, Wuhan 430072, China

Received:2009-08-10 Revised:2009-10-20 Online:2010-03-20 Published:2010-03-15
Contact: SHE Chao-Wen E-mail:shechaowen@tom.com

摘要/Abstract

摘要：

为分析玉米着丝粒卫星DNA(CentC)和着丝粒反转录转座子(CRM)在玉米种的亚种及近缘种中的保守性,采用双色荧光原位杂交检测了这两种重复序列在墨西哥玉米、二倍体多年生类玉米、多年生类玉米、摩擦禾、薏苡、高粱中的存在和分布。CentC、CRM探针在墨西哥玉米、二倍体多年生类玉米和多年生类玉米的所有染色体的着丝粒区产生了强或较强的杂交信号, 而且不同染色体的杂交信号的强度存在差异, 表明两种玉米着丝粒重复序列在不同着丝粒中的数量不同; 此外, 部分着丝粒中的CentC和CRM信号的强度存在差异, 不完全重叠。CentC、CRM探针仅在摩擦禾的多数染色体的着丝粒区产生了弱的杂交信号。在薏苡和高粱中仅测检到主要分布在着丝粒区的较强或强的CRM信号。这些结果表明, CentC在玉米种的亚种间及玉蜀黍属的物种间高度保守, 在与玉蜀黍属亲缘关系最近的摩擦禾属物种中也具有较高的保守性; CRM在与玉蜀黍属亲缘关系较近和较远的禾本科种属中都具有保守性。

关键词: 玉蜀黍属, 着丝粒, 玉米着丝粒卫星DNA, 玉米着丝粒反转录转座子, 荧光原位杂交

Abstract:

In order to analyze the conservation of maize centromeric satellite DNA (CentC) and centromeric retrotransposon (CRM) in the subspecies and relatives of Zea mays, dual fluorescence in situ hybridization (FISH) was used to detect the existence and distribution of the above two repetitive sequences in Zea mays ssp. mexicana, Z. diploperennis, Z. perennis, Tripsacum dactyloides, Coix lacryma-jobi, and Sorghum bicolor. In Z. mays ssp. mexicana, Z. diploperennis, and Z. perennis, both CentC and CRM probes produced strong or relatively strong signals in the centromeric regions of all chromosomes. There was an obvious variation in the intensity of hybridization signals on different chromosomes, indicating that different centromeres have different amounts of CentC and CRM sequences. In some centromeres, the intensity of CentC signals differed from that of CRM signals and was free from overlapping. In T. dactyloides, only weak CentC and CRM signals were detected in the centromeric regions of most chromosomes, while in C. lacryma-jobi and S. bicolor only relatively strong or strong CRM signals primarily located in the centromeric regions were detected. This result indicates that CentC is highly conserved among the subspecies of Z. mays and the species of Zea, and has high conservation in Tripsacum, a genus that is most closely related to Zea, and CRM is conserved among the species of grass family either closely or distantly related to Zea.

Key words: Zea, centromere, CentC, CRM, fluorescence in situ hybridization

佘朝文，蒋向辉，宋运淳，刘伟. 玉米近缘种中玉米着丝粒序列的FISH检测[J]. 遗传, 2010, 32(3): 264-270.

SHE Chao-Wen, JIANG Xiang-Hui, SONG Yun-Chun, LIU Wei. Detection of maize centromeric repeats in the relatives of maize using fluorescence in situ hybridization[J]. HEREDITAS, 2010, 32(3): 264-270.

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玉米近缘种中玉米着丝粒序列的FISH检测

Detection of maize centromeric repeats in the relatives of maize using fluorescence in situ hybridization

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