大鼠再生肝8种细胞的丝氨酸族氨基酸代谢相关基因的转录谱

doi:10.3724/SP.J.1005.2010.00829

遗传 ›› 2010, Vol. 32 ›› Issue (8): 829-838.doi: 10.3724/SP.J.1005.2010.00829

大鼠再生肝8种细胞的丝氨酸族氨基酸代谢相关基因的转录谱

常翠芳^1,2,王改平²,朱秋实²,王磊²,张富春¹,马纪¹,徐存拴^2,3

1. 新疆大学生命科学与技术学院, 乌鲁木齐 830046; 2. 河南省-科技部共建细胞分化调控重点实验室, 新乡 453007; 3. 河南师范大学生命科学学院, 新乡 453007

收稿日期:2009-11-12 修回日期:2010-03-06 出版日期:2010-08-20 发布日期:2010-08-23
通讯作者: 徐存拴 E-mail:xucs@x263.net
基金资助:
国家973项目前期研究专项(编号：2006CB708506)资助

Transcriptome atlas of serine family amino acid metabolism-related genes in eight rat regenerating liver cell types

CHANG Cui-Fang^{1, 2}, WANG Gai-Ping², ZHU Qiu-Shi², WANG Lei², ZHANG Fu-Chun¹, MA Ji¹, XU Cun-Shuan^2,3

1. College of Life Science and Technology, Xin Jiang University, Urumqi 830046, China; 2. Co-construction Key Laboratory for Cell Differentiation and Regulation, Xinxiang 453007, China; 3. College of Life Science, Henan Normal University, Xinxiang 453007, China

Received:2009-11-12 Revised:2010-03-06 Online:2010-08-20 Published:2010-08-23
Contact: XU Cun-Shuan E-mail:xucs@x263.net

摘要/Abstract

摘要： 为了解大鼠肝再生中8种肝脏细胞的丝氨酸族氨基酸代谢相关基因转录谱, 文章用Percoll密度梯度离心结合免疫磁珠分选分离大鼠的8种再生肝细胞, 用Rat Genome 230 2.0芯片等检测它们中丝氨酸族氨基酸代谢相关基因的表达变化, 用Cluster和Treeview等软件分析上述基因在肝再生中表达模式, 用生物信息学和系统生物学等方法分析上述细胞中丝氨酸族氨基酸代谢活动。结果表明, 在27个发生有意义表达变化的基因中, 肝细胞、胆管上皮细胞、卵圆细胞、肝星形细胞、窦内皮细胞、库普弗细胞、陷窝细胞、树突状细胞的基因数分别为13、16、11、14、13、11、12、14, 相应细胞的上调、下调和上/下调的基因数分别为7、6和0, 2、10和4, 2、8和1, 8、3和3, 6、5和2, 4、6和1, 2、10和0, 6、6和2。总的来看, 肝再生中各细胞的表达下调基因占优势, 但在肝再生启动阶段, 肝星形细胞和窦内皮细胞的表达上调基因占优势。上述丝氨酸族氨基酸代谢相关基因转录谱预示丝氨酸族氨基酸的合成主要在肝再生启动阶段的肝细胞、肝星形细胞、窦内皮细胞和库普弗细胞中增强, 它们的降解主要在肝再生进展阶段的肝细胞、胆管上皮细胞、陷窝细胞和树突状细胞中进行。

关键词: 肝再生, Rat Genome 230 2.0芯片, 甘氨酸, 丝氨酸, 半胱氨酸

Abstract: To explore the transcription profiles of serine family amino acid metabolism-related genes in eight liver cell types during rat liver regeneration (LR), eight types of rat regenerating liver cells were isolated using the combination of percoll density gradient centrifugation and immunomagnetic bead methods. Then, the expression profiles of the genes associated with metabolism of serine family amino acid in rat liver regeneration were detected by Rat Genome 230 2.0 Array. The expression patterns of these genes were analyzed through the software of Cluster and Treeview. The activities of serine family amino acid metabolism were analyzed by the methods of bioinformatics and systems biology. The results showed that 27 genes were significantly expressed. Among them, the numbers of genes showing significant expression changes in hepatocytes, biliary epithelial cells, oval cells, hepatic stellate cells, sinusoidal endothelial cells, Kupffer cells, pit cells and dendritic cells were 13, 16, 11, 14, 13, 11, 12, and 14, respectively. The numbers of up-, down-, and up-/down-regulated genes in corresponding cells were 7, 6, and 0; 2, 10, and 4; 2, 8, and 1; 8, 3, and 3; 6, 5, and 2; 4, 6, and 1; 2, 10, and 0; and 6, 6, and 2. Overall, the genes in the eight types of cells were mostly down-regulated during liver regeneration, but most LR-related genes in hepatic stellate cells and sinusoidal endothelial cells were up-regulated in priming phase. It is suggested that biosynthesis of serine family amino acid was enhanced in hepatocytes, hepatic stellate cells, sinusoidal endothelial cells and Kupffer cells in the priming phase. The catabolism of them was enhanced in hepatocytes, biliary epithelial cells, pit cells and dendritic cells in progressive phase.

Key words: rat liver regeneration, Rat Genome 230 2.0 Array, glycine, serine, cysteine

常翠芳，王改平，朱秋实，王磊，张富春，马纪，徐存拴. 大鼠再生肝8种细胞的丝氨酸族氨基酸代谢相关基因的转录谱[J]. 遗传, 2010, 32(8): 829-838.

CHANG Cui-Fang, WANG Gai-Beng, SHU Qiu-Shi, WANG Lei, ZHANG Fu-Chun, MA Ji, XU Cun-Quan. Transcriptome atlas of serine family amino acid metabolism-related genes in eight rat regenerating liver cell types[J]. HEREDITAS, 2010, 32(8): 829-838.

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大鼠再生肝8种细胞的丝氨酸族氨基酸代谢相关基因的转录谱

Transcriptome atlas of serine family amino acid metabolism-related genes in eight rat regenerating liver cell types

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