全基因组扩增技术及其在法医个体识别中的应用

doi:10.3724/SP.J.1005.2010.01119

遗传 ›› 2010, Vol. 32 ›› Issue (11): 1119-1125.doi: 10.3724/SP.J.1005.2010.01119

全基因组扩增技术及其在法医个体识别中的应用

蔡海强¹, 柳海涛², 史斌², 李安², 唐文如¹, 罗瑛¹

1. 昆明理工大学生命科学与技术学院衰老与肿瘤分子遗传实验室, 昆明 650224; 2. 昆明市公安局刑事犯罪侦查支队, 昆明 650224

收稿日期:2010-01-23 修回日期:2010-03-29 出版日期:2010-11-20 发布日期:2010-11-25
通讯作者: 罗瑛;唐文如 E-mail:luoyingabc@yahoo.com;twr@sina.com
基金资助:
云南省科技计划项目(编号：2009CA012)资助

Recent Advance of Whole Genome Amplification and its Application prospect in Forensic Individual Identification

CAI Hai-Qiang¹, LIU Hai-Tao², SHI Bin², LI An², TANG Wen-Ru¹, LUO Ying¹

1. Laboratory of Molecular Genetics of Aging and Tumor, Faculty of Life Science and Technology, Kunming University of Science and Technology, Kunming 650224, China; 2. Criminal investigation team, Kunming public security bureau, Kunming 650224, China

Received:2010-01-23 Revised:2010-03-29 Online:2010-11-20 Published:2010-11-25
Contact: LUO Ying;TANG Wen-Ru E-mail:luoyingabc@yahoo.com;twr@sina.com

摘要/Abstract

摘要： 全基因组扩增(Whole genome amplification, WGA)技术是一种对全部基因组序列进行非选择性扩增的技术。近几年来, 对 WGA技术扩增痕量DNA检材的研究日渐深入, 这些研究可望用于刑案现场采集到的痕量DNA样品的扩增, 为法医个体识别提供足量的DNA模板。然而, 对实际案件中复杂检材的扩增偏差问题一直困扰着法医工作者, 寻求一个低扩增偏差、高扩增产率的WGA技术是法医工作者的主要目标。文章综述了WGA技术在法医个体识别中的研究进展及应用前景, 为法医解决扩增偏差问题提供参考。

关键词: 全基因组扩增, STR, 个体识别, 法医

Abstract: Whole genome amplification (WGA) is a technique that could non-selectively amplify the genomic DNA. In recent years, intensive studies have been made on application of WGA in trace DNA amplification, which might be applied to amplify trace DNA collected from crime scenes and provide enough DNA templates for forensic individual identification. However, the problem of amplification bias happened in the complicated test materials from real crime scenes is still troublesome. Development of WGA method with low amplification bias and high yield is the main target of current forensic research. Here, we reviewed the advances and the application prospect of WGA in individual identification. This might provide a reference for solving the problem of the amplification bias.

Key words: whole genome amplification (WGA), short tandem repeat, individual identification, forensic science

蔡海强，柳海涛，史斌，李安，唐文如，罗瑛. 全基因组扩增技术及其在法医个体识别中的应用[J]. 遗传, 2010, 32(11): 1119-1125.

CA Hai-Jiang, LIU Hai-Chao, SHI Bin, LI An, TANG Wen-Ru, LUO Ying. Recent Advance of Whole Genome Amplification and its Application prospect in Forensic Individual Identification[J]. HEREDITAS, 2010, 32(11): 1119-1125.

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全基因组扩增技术及其在法医个体识别中的应用

Recent Advance of Whole Genome Amplification and its Application prospect in Forensic Individual Identification

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