Cre/lox位点特异性重组系统在高等真核生物中的研究进展

doi:10.3724/SP.J.1005.2012.00177

遗传 ›› 2012, Vol. 34 ›› Issue (2): 177-189.doi: 10.3724/SP.J.1005.2012.00177

Cre/lox位点特异性重组系统在高等真核生物中的研究进展

龙定沛¹, 谭兵^1,2, 赵爱春¹, 许龙霞^1,3, 向仲怀¹

1. 西南大学蚕学与系统生物学研究所, 农业部蚕学重点开放实验室, 重庆 400716 2. 中国科学院武汉病毒研究所, 分子病毒学及病毒免疫学学科组, 武汉 430071 3. 中国科学院上海生命科学研究院, 生物化学与细胞生物学研究所, 上海 200031

收稿日期:2011-06-03 修回日期:2011-09-07 出版日期:2012-02-20 发布日期:2012-02-25
通讯作者: 赵爱春 E-mail:zhaoaichun@hotmail.com
基金资助:
重庆市自然科学基金计划重点项目(编号: 2011BA1005), 中央高校基本科研业务费专项(编号: XDJK2010B015)和中国博士后科学基金项目(编号: 20070420722和200801221)资助

Progress in Cre/lox site-specific recombination system in higher eukaryotes

LONG Ding-Pei¹, TAN Bing^1,2, ZHAO Ai-Chun¹, XU Long-Xia^1,3, XIANG Zhong-Huai¹

1. Institute of Sericulture and Systems Biology, Key Sericultural Laboratory of the Ministry of Agriculture, Southwest University, Chongqing 400716, China 2. Molecular Virology and Viral Immunology Research Group, Wuhan Institute of Virology, Chinese Academy of Sciences, Wuhan 430071, China 3. Shanghai Institute of Biological Sciences, Institute of Biochemistry and Cell Biology, Shanghai 200031, China

Received:2011-06-03 Revised:2011-09-07 Online:2012-02-20 Published:2012-02-25

摘要/Abstract

摘要： 来自于P1噬菌体的Cre/lox系统通过位点特异性重组可以迅速而有效地实现各种生理环境下的基因定点插入、删除、替换和倒位等操作。Cre/lox系统作为目前基因打靶技术的核心工具, 已被广泛应用于拟南芥、水稻、小鼠、果蝇、斑马鱼等高等真核模式生物。文章较为全面地介绍了Cre/lox系统的基本概况及其在高等真核生物中的应用, 讨论了Cre/lox系统在研究中存在的主要问题和今后的发展方向, 为利用该系统在不同高等生物中进行基因操作提供有用的参考。

关键词: Cre/lox重组系统, 基因操作, 位点特异性重组, 基因打靶

Abstract: Cre/lox system derived from P1 bacteriaphage can quickly and effectively achieve gene insertion, deletion, replacement, and inversion by means of site-specific recombination. As one of the most important tools for gene targeting at present, Cre/lox system has been widely used in Arabidopsis thaliana, Oryza sativa L., Mus musculus, Drosophila melanogaster, Danio rerio, and other higher eukaryotic organisms. This review roundly described the basic profile of Cre/lox system, and its application in higher eukaryotes. In addition, we also discussed the main problems and developmental trend of the Cre/lox system in this review, which can be a good reference for using Cre/lox system to realize the gene manipulations of the different high eukaryotic organisms.

Key words: Cre/lox recombination system, gene manipulation, site-specific recombination, gene targeting

龙定沛，谭兵，赵爱春，许龙霞，向仲怀. Cre/lox位点特异性重组系统在高等真核生物中的研究进展[J]. 遗传, 2012, 34(2): 177-189.

LONG Ding-Pei, TAN Bing, ZHAO Ai-Chun, XU Long-Xia, XIANG Zhong-Huai. Progress in Cre/lox site-specific recombination system in higher eukaryotes[J]. HEREDITAS, 2012, 34(2): 177-189.

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Cre/lox位点特异性重组系统在高等真核生物中的研究进展

Progress in Cre/lox site-specific recombination system in higher eukaryotes

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