遗传 ›› 2012, Vol. 34 ›› Issue (2): 177-189.doi: 10.3724/SP.J.1005.2012.00177
龙定沛1, 谭兵1,2, 赵爱春1, 许龙霞1,3, 向仲怀1
收稿日期:2011-06-03
修回日期:2011-09-07
出版日期:2012-02-20
发布日期:2012-02-25
通讯作者:
赵爱春
E-mail:zhaoaichun@hotmail.com
基金资助:重庆市自然科学基金计划重点项目(编号: 2011BA1005), 中央高校基本科研业务费专项(编号: XDJK2010B015)和中国博士后科学基金项目(编号: 20070420722和200801221)资助
LONG Ding-Pei1, TAN Bing1,2, ZHAO Ai-Chun1, XU Long-Xia1,3, XIANG Zhong-Huai1
Received:2011-06-03
Revised:2011-09-07
Online:2012-02-20
Published:2012-02-25
摘要： 来自于P1噬菌体的Cre/lox系统通过位点特异性重组可以迅速而有效地实现各种生理环境下的基因定点插入、删除、替换和倒位等操作。Cre/lox系统作为目前基因打靶技术的核心工具, 已被广泛应用于拟南芥、水稻、小鼠、果蝇、斑马鱼等高等真核模式生物。文章较为全面地介绍了Cre/lox系统的基本概况及其在高等真核生物中的应用, 讨论了Cre/lox系统在研究中存在的主要问题和今后的发展方向, 为利用该系统在不同高等生物中进行基因操作提供有用的参考。
龙定沛，谭兵，赵爱春，许龙霞，向仲怀. Cre/lox位点特异性重组系统在高等真核生物中的研究进展[J]. 遗传, 2012, 34(2): 177-189.
LONG Ding-Pei, TAN Bing, ZHAO Ai-Chun, XU Long-Xia, XIANG Zhong-Huai. Progress in Cre/lox site-specific recombination system in higher eukaryotes[J]. HEREDITAS, 2012, 34(2): 177-189.
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