遗传 ›› 2011, Vol. 33 ›› Issue (5): 449-458.doi: 10.3724/SP.J.1005.2011.00449
罗庆苗, 苗向阳, 张瑞杰
收稿日期:
2010-11-18
修回日期:
2011-03-16
出版日期:
2011-05-20
发布日期:
2011-05-25
通讯作者:
苗向阳
E-mail:mxy32@sohu.com
基金资助:
转基因生物新品种培育科技重大专项(编号:2009ZX08008-004B, 2008ZX08008-003), 国家高技术研究发展计划(863计划)项目 (编号:2008AA10Z140), 国家自然科学基金项目(编号:30571339)和中国农业科学院创新基金项目(编号:2004-院-1)资助
LUO Qing-Miao, MIAO Xiang-Yang, ZHANG Rui-Jie
Received:
2010-11-18
Revised:
2011-03-16
Online:
2011-05-20
Published:
2011-05-25
Contact:
MIAO Xiang-Yang
E-mail:mxy32@sohu.com
摘要: 转基因技术经过数十年的发展日渐成熟, 并推动转基因技术研究进入一个新的发展阶段。文章综述了体细胞核移植技术、慢病毒载体法、转座子介导的基因转移法、RNA干扰介导的基因敲除法和锌指核酸酶-基因打靶技术等近年发展起来的方法。而近来诱导多能干细胞(iPS 细胞)的成功为尚未获得ES细胞的大动物建立多能干细胞系提供了一种新的方案, 为转基因动物研究开创一片更广阔的天地。文章在前人研究的基础上重点总结了以上各种转基因技术的最新研究动态并对各种转基因技术的特点进行了探讨。
罗庆苗,苗向阳,张瑞杰. 转基因动物新技术研究进展[J]. 遗传, 2011, 33(5): 449-458.
LUO Qiang-Miao, MIAO Xiang-Yang, ZHANG Rui-Jie. An update on the development of transgenic animal technology[J]. HEREDITAS, 2011, 33(5): 449-458.
[1] Gordon JW, Scangos GA, Plotkin DJ, Barbosa JA, Ruddle FH. Genetic transformation of mouse embryos by microinjection of purified DNA. Proc Natl Acad Sci USA, 1980, 77(12): 7380-7384.[2] Palmiter RD, Brinster RL, Hammer RE. Dramatic growth of mice that develop from eggs microinjected with metallothionein-growth hormone fusion genes. Nature, 1982, 300(5893): 611-615.[3] Hammer RE, Pursel VG, Rexroad CE Jr, Wall RJ, Bolt DJ, Ebert KM, Palmiter RD, Brinster RL. Production of transgenic rabbits, sheep and pigs by microinjection. Nature, 1985, 315(6021): 680-683.[4] Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KHS. Viable offspring derived from fetal and adult mammalian cells. Nature, 1997, 385(6619): 810-813.[5] 吴昭, 成璐, 肖磊. 新物种诱导多能干细胞的研究进展. 生命科学, 2009, 21(5): 658-662.[6] Schnieke AE, Kind AJ, Ritchie WA, Mycock K, Scott AR, Ritchie M, Wilmut I, Colman A, Campbell KHS. Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science, 1997, 278 (5346): 2130-2133.[7] Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, de León FAP, Robl JM. Transgenic bovine chimeric offspring produced from somatic cell-derived stem-like cells. Nat Biotechnol, 1998, 16(7): 642-646.[8] Macháty Z, Bondioli KR, Ramsoondar JJ, Fodor WL. The use of nuclear transfer to produce transgenic pigs. Cloning Stem Cells, 2002, 4(1): 21-27.[9] 龚国春, 戴蕴平, 樊宝良, 朱化彬, 王莉莉, 王海平, 汤波, 刘颖, 李荣, 万荣, 黄银花, 李宁. 利用体细胞核移植技术生产转基因牛. 科学通报, 2003, 48(24): 32-37.[10] 张运海, 潘登科, 孙秀柱, 孙国杰, 王晓波, 刘晓辉, 李燕, 戴蕴平, 李宁. 利用体细胞核移植技术生产表达绿色荧光蛋白的猪转基因克隆胚胎. 中国科学C辑: 生命科学, 2005, 35(5): 439-445.[11] Yang PH, Wang JW, Gong GC, Sun XZ, Zhang R, Du Z, Liu Y, Ding FR, Tang B, Dai YP, Li N. Cattle mammary bioreactor generated by a novel procedure of transgenic cloning for large-scale production of functional human lactoferrin. PLoS One, 2008, 3(10): e3453.[12] Yang DS, Wang CE, Zhao BT, Li W, Ouyang Z, Liu ZM, Yang HQ, Fan P, O'Neill A, Gu WW, Yi H, Li SH, Lai LX, Li XJ. Expression of Huntington's disease protein results in apoptotic neurons in the brains of cloned transgenic pigs. Hum Mol Genet, 2010, 19(20): 3983-3994.[13] Pfeifer A, Ikawa M, Dayn Y, Verma IM. Transgenesis by lentiviral vectors: lack of gene silencing in mammalian embryonic stem cells and preimplantation embryos. Proc Natl Acad Sci USA, 2002, 99(4): 2140-2145.[14] Lois C, Hong EJ, Pease S, Brown EJ, Baltimore D. Germline transmission and tissue-specific expression of transgenes delivered by Lentiviral Vectors. Science, 2002, 295(5556): 868-872.[15] Hofmann A, Kessler B, Ewerling S, Weppert M, Vogg B, Ludwig H, Stojkovic M, Boelhauve M, Brem G, Wolf E, Pfeifer A. Eficient transgenesis in farm animals by lentiviral vectors. EMBO Rep, 2003, 4(11): 1054-1058.[16] MeGrew MJ, Sherman A, Ellard FM, Lillico SG, Gihooley HJ, Kingsman AJ, Mitrophanous KA, Sang H. Eficient production of germline transgenie chickens using lentiviral vectors. EMBO Rep, 2004, 5(7): 728-733.[17] Sasaki E, Suemizu H, Shimada A, Hanazawa K, Oiwa R, Kamioka M, Tomioka I, Sotomaru Y, Hirakawa R, Eto T, Shiozawa S, Maeda T, Ito M, Ito R, Kito C, Yagihashi C, Kawai K, Miyoshi H, Tanioka Y, Tamaoki N, Habu S, Okano H, Nomura T. Generation of transgenic non-human primates with germline transmission. Nature, 2009, 459(7246): 523-527.[18] 张敬之, 郭歆冰, 谢书阳, 朱怡文, 黄英, 王舒, 任兆瑞. 用慢病毒载体介导产生绿色荧光蛋白(GFP)转基因小鼠. 自然科学进展, 2006, 16(5): 571-577.[19] Niu YY, Yu Y, Bernat A, Yang SH, He XC, Guo XY, Chen DL, Chen YC, Ji SH, Si W, Lv YQ, Tan T, Wei Q, Wang H, Shi L, Guan J, Zhu XM, Afanassieff M, Savatier P, Zhang K, Zhou Q, Ji WZ. Transgenic rhesus monkeys produced by gene transfer into early-cleavage-stage embryos using a simian immunodeficiency virus-based vector. Proc Natl Acad Sci USA, 2010, 107(41): 17663-17667.[20] Fadool JM, Hartl DL, Dowling JE. Transposition of the mariner element from Drosophila mauritiana in zebrafish. Proc Natl Acad Sci USA, 1998, 95(9): 5182-5186.[21] Sherman A, Dawson A, Mather C, Gilhooley H, Li Y, Mitchell R, Finnegan D, Sang H. Transposition of the Drosophila element mariner into the chicken germ line. Nat Biotechnol, 1998, 16(11): 1050-1053.[22] Ivics Z, Hackett PB, Plasterk RH, Izsvák Z. Molecular reconstruction of Sleeping Beauty, a Tc1-like transposon from fish, and its transposition in human cells. Cell, 1997, 91(4): 501-510.[23] Dupuy AJ, Clark K, Carlson CM, Fritz S, Davidson AE, Markley KM, Finley K, Fletcher CF, Ekker SC, Hackett PB, Horn S, Largaespada DA. Mammalian germ-line transgenesis by transposition. Proc Natl Acad Sci USA, 2002, 99(7): 4495-4499.[24] Ding S, Wu XH, Li G, Han M, Zhuang Y, Xu T. Efficient transposition of the piggyBac(PB) transposon in mammalian cells and mice. Cell, 2005, 122(3): 473-483.[25] Wu S, Ying GX, Wu Q, Capecchi MR. Toward simpler and faster genome-wide mutagenesis in mice. Nat Genet, 2007, 39(7): 922-930.[26] Woltjen K, Michael IP, Mohseni P, Desai R, Mileikovsky M, Hämäläinen R, Cowling R, Wang W, Liu PT, Gertsenstein M, Kaji K, Sung HK, Nagy A. piggyBac transposition reprograms fibroblasts to induced pluripotent stem cells. Nature, 2009, 458(7239): 766-770.[27] Klattenhoff C, Xi HL, Li CJ, Lee S, Xu J, Khurana JS, Zhang F, Schultz N, Koppetsh BS, Nowosielska A, Seitz H, Zamore PD, Weng ZP, Theurkauf WE. The Drosophila HP1 homolog rhino is required for transposon silencing and piRNA production by dual-strand clusters. Cell, 2009, 138(6): 1137-1149.[28] Fire A, Xu SQ, Montgomery MK, Kostas SA, Driver SE, Mello CC. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature, 1998, 391(6669): 806-811.[29] Elbashir SM, Harborth J, Lendeckel W, Yalcin A, Weber K, Tusch T. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells. Nature, 2001, 411(6836): 494-498.[30] Hasuwa H, Kaseda K, Einarsdottir T, Okabe M. Small interfering RNA and gene silencing in transgenic mice and rats. FEBS Lett, 2002, 532 (1-2): 227-230.[31] Ramsoondar J, Vaught T, Ball S, Mendicino M, Monahan J, Jobst P, Vance A, Duncan J, Wells K, Ayares D. Production of transgenic pigs that express porcine endogenous retrovirus small interfering RNAs. Xenotransplantation, 2009, 16(3): 164-180.[32] 杨志峰, 王龙, 杨生生, 匡颖, 陈欢, 徐国江, 蔡在龙, 王铸钢, 毛积芳. 慢病毒载体介导IκB激酶-α(IKKα)基因RNA干扰转基因小鼠的制备. 生物化学与生物物理进展, 2007, 34(2): 215-221.[33] Li Y, Li H, Yao G, Li W, Wang F, Jiang Z, Li M. Inhibition of telomerase RNA (hTR) in cervical cancer by adenovirus-delivered siRNA. Cancer Gene Ther, 2007, 14(8): 748-755.[34] Thomas KR, Capecchi MR. Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell, 1987, 51(3): 503-512.[35] McCreath KJ, Howcroft J, Campbell KHS, Colman A, Schnieke AE, Kind AJ. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature, 2000, 405(6790): 1066-1069.[36] Lai LX, Kolber-Simonds D, Park KW, Cheong HT, Greenstein JL, Im GS, Samuel M, Bonk A, Rieke A, Day BN, Murphy CN, Carter DB, Hawley RJ, Prather RS. Production of α-1, 3-galactosyltransferase knockout pigs by nuclear transfer cloning. Science, 2002, 295(5557): 1089-1092.[37] Kuroiwa Y, Kasinathan P, Matsushita H, Sathiyaselan J, Sullivan EJ, Kakitani M, Tomizuka K, Ishida I, Robl JM. Sequential targeting of the genes encoding immunoglobulin-μ and prion protein in cattle. Nat Genet, 2004, 36(7): 775-780.[38] van de Lavoir MC, Diamond JH, Leighton PA, Mather-Love C, Heyer BS, Bradshaw R, Kerchner A, Hooi LT, Gessaro TM, Swanberg SE, Delany ME, Etches RJ. Germline transmission of genetically modified primordial germ cells. Nature, 2006, 441(7094): 766-769.[39] Tong C, Li P, Wu NL, Yan YZ, Ying QL. Production of p53 gene knockout rats by homologous recombination in embryonic stem cells. Nature, 2010, 467(7312): 211-213.[40] Meng XD, Noyes MB, Zhu LJ, Lawson ND, Wolfe SA. Targeted gene inactivation in zebrafish using engineered zinc-finger nucleases. Nat Biotechnol, 2008, 26(6): 695-701.[41] Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, Unger-Wallace E, Sander JD, Müller-Lerch F, Fu FL, Pearlberg J, Göbel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Johnlafrate A, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK. Rapid “open-source” engineering of customized zinc-finger nucleases for highly efficient gene modification. Mol Cell, 2008, 31(2): 294-301.[42] Geurts AM, Cost GJ, Freyvert Y, Zeitler B, Miller JC, Choi VM, Jendins SS, Wood A, Cui XX, Meng XD, Vincent A, Lam S, Michalkiewicz M, Schilling R, Foeckler J, Kalloway S, Weiler H, Ménoret S, Anegon I, Davis GD, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jacob HJ, Buelow R. Knockout rats via embryo microinjection of zinc-finger nucleases. Science, 2009, 325(5939): 433-433.[43] Mashimo T, Takizawa A, Voigt B, Yoshimi K, Hiai H, Kuramoto T, Serikawa T. Generation of knockout rats with X-linked severe combined immunodeficiency(X-SCID) using zinc-finger nucleases. PLoS One, 2010, 5(1): e8870.[44] Carbery ID, Ji D, Harrington A, Brown V, Weinstein EJ, Liaw L, Cui XX. Targeted genome modification in mice using zinc-finger nucleases. Genetics, 2010, 186(2): 451-459.[45] Gupta A, Meng XD, Zhu LJ, Lawson ND, Wolfe SA. Zinc finger protein-dependent and -independent contributions to the in vivo off-target activity of zinc finger nucleases. Nucleic Acids Res, 2011, 39(1): 381-392.[46] Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 2006, 126(4): 663-676.[47] Kaji K, Norrby K, Paca A, Mileikovsky M, Mohseni P, Woltjen K. Virus-free induction of pluripotency and subsequent excision of reprogramming factors. Nature, 2009, 458(7239): 771-775.[48] Soldner F, Hockemeyer D, Beard C, Gao Q, Bell GW, Cook EG, Hargus G, Blak A, Cooper O, Mitalipova M, Isacson O, Jaenisch R. Parkinson’s disease patient-derived induced pluripotent stem cells free of viral reprogramming factors. Cell, 2009, 136(5): 964-977.[49] Kim K, Doi A, Wen B, Ng K, Zhao R, Cahan P, Kim J, Aryee MJ, Ji H, Ehrlich LIR, Yabuuchi A, Takeuchi A, Cunniff KC, Hongguang H, Mckinney-Freeman S, Naveiras O, Yoon TJ, Irizarry RA, Jung N, Seita J, Hanna J, Murakami P, Jaenisch R, Weissleder R, Orkin SH, Weissman IL Feinberg AP, Daley GQ. Epigenetic memory in induced pluripotent stem cells. Nature, 2010, 467(7313): 285-290.[50] Polo JM, Liu S, Figueroa ME, Kulalert W, Eminli S, Tan KY, Apostolou E, Stadtfeld M, Li YS, Shioda T, Natesan S, Wagers AJ, Melnick A, Evans T, Hochedlinger K. Cell type of origin influences the molecular and functional properties of mouse induced pluripotent stem cells. Nat Biotechnol, 2010, 28(8): 848-855.[51] Liu HS, Zhu FF, Yong J, Zhang PB, Hou PP, Li HG, Jiang W, Cai J, Liu M, Cui K, Qu XX, Xiang TT, Lu DY, Chi XC, Gao G, Ji WZ, Ding MX, Deng HK. Generation of induced pluripotent stem cells from adult rhesus monkey fibroblasts. Cell Stem Cell, 2008, 3(6): 587-590.[52] Wu Z, Chen JJ, Ren JT, Bao L, Liao J, Cui C, Rao LJ, Li H, Gu YJ, Dai HM, Zhu H, Teng XK, Cheng L, Xiao L. Generation of pig induced pluripotent stem cells with a drug-inducible system. J Mol Cell Biol, 2009, 1(1): 46-54.[53] Esteban MA, Xu JY, Yang JY, Peng MX, Qin DJ, Li W, Jiang ZX, Chen JK, Deng K, Zhong M, Cai JL, Lai LX, Pei DQ. Generation of induced pluripotent stem cell lines from Tibetan miniature pig. J Biol Chem, 2009, 284(26): 17634-17640.[54] Zhao XY, Li W, Lv Z, Liu L, Tong M, Hai T, Hao J, Guo CL, Ma QW, Wang L, Zeng F, Zhou Q. iPS cells produce viable mice through tetraploid complementation. Nature, 2009, 461(7260): 86-90.[55] Kang L, Wang JL, Zhang Y, Kou ZH, Gao SR. iPS cells can support full-term development of tetraploid blastocyst-complemented embryos. Cell Stem Cell, 2009, 5(2): 135-138.[56] Hockemeyer D, Soldner F, Beard C, Gao Q, Mitalipova M, DeKelver RC, Katibah GE, Amora R, Boydston EA, Zeitler B, Meng XD, Miller JC, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jaenisch R. Efficient targeting of expressed and silent genes in human ESCs and iPSCs using zinc-finger nucleases. Nat Biotechnol, 2009, 27(9): 851-857. |
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