建鲤(Cyprinus carpio var. Jian)微卫星DNA亲权鉴定

doi:10.3724/SP.J.1005.2012.01447

遗传 ›› 2012, Vol. 34 ›› Issue (11): 1447-1455.doi: 10.3724/SP.J.1005.2012.01447

建鲤(Cyprinus carpio var. Jian)微卫星DNA亲权鉴定

顾颖^1, 李超¹, 鲁翠云¹, 郑先虎¹, 俞菊华², 孙效文¹

1. 中国水产科学研究院黑龙江水产研究所, 农业部淡水水产生物技术与遗传育种重点实验室, 哈尔滨 150070 2. 中国水产科学研究院淡水渔业研究中心, 无锡 214081

收稿日期:2012-03-28 修回日期:2012-05-12 出版日期:2012-11-20 发布日期:2012-11-25
通讯作者: 孙效文 E-mail:sunxw2002@163.com
基金资助:
公益性行业(农业)科研专项(编号：200903045), 国家重点基础研究发展计划项目(编号：2010CB126305)和农业部“引进国际先进农业科学技术”重点项目(编号：2011-G12) 资助

Microsatellite markers for parentage identification in Jian Carp (Cyprinus carpio var. Jian)

GU Ying¹, LI Chao¹, LU Cui-Yun¹, ZHENG Xian-Hu¹, YU Ju-Hua², SUN Xiao-Wen¹

1. Freshwater Fisheries Biotechnology and Genetic Breeding Key Lab of Ministry of Agriculture, Heilongjiang Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Harbin 150070, China 2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China

Received:2012-03-28 Revised:2012-05-12 Online:2012-11-20 Published:2012-11-25

摘要/Abstract

摘要： 利用16个微卫星座位对建鲤10个全同胞家系647个子代进行亲权鉴定。Cervus3.0分析表明, 16个微卫星位点的平均多态信息含量为0.7025, 平均等位基因数为6.63, 期望杂合度平均为0.7405。当双亲未知时, 累积排除概率为0.999225, 已知单亲时的累积排除概率为0.999996, 置信度为95%。进一步模拟分析表明, 要达到亲权鉴定的要求在双亲未知时通常需要8～12个微卫星位点, 已知单亲时需要5～8个微卫星位点。在双亲均未知的情况下进行亲权鉴定, 94.6%的后裔找到了其父母本, 真实鉴定率低于模拟分析预测值, 分析可能是与候选亲本间存在亲缘关系、无效等位基因的存在以及分型错误等因素有关。9个建鲤全同胞家系的鉴定, 为今后的遗传图谱构建、QTL定位及分子标记辅助育种研究奠定了基础。

关键词: 建鲤, 亲权鉴定, 微卫星

Abstract: Using 16 microsatellite loci we estimated the parentage of 647 progeny in 10 Jian Carp full-sib families. Cervus 3.0 analysis showed that mean PIC value of 16 microsatellites, mean number of allele, and mean expected heterozygosity were 0.7025, 6.63, and 0.7405, respectively. The combined probability of exclusion was 0.99922456 when both parents were unknown and the combined probability of exclusion was 0.99999557 when only one of the parental genotype was known, with the confidence level of 95%. Further simulations based on allele frequencies suggested that to achieve the requirements of paternity test usually took 8 to 12 microsatellite loci when both parents were unknown and 5 to 8 microsatellite loci when one parent was known. Out of 647 progenies, 94.6% were assigned to their parental pairs without the information of both parents in parentage analysis, which were lower than the theoretical assignment rates predicted by the Cervus simulations. This could be explained by the relationship between the candidate parents or existence of null and by typing errors. The identification of 9 families was useful for linkage analysis of Jian Carp and QTL location, also for marker assisted selection for economical traits.

Key words: Jian Carp, parental identification, microsatellite

顾颖，李超，鲁翠云，郑先虎，俞菊华，孙效文. 建鲤(Cyprinus carpio var. Jian)微卫星DNA亲权鉴定[J]. 遗传, 2012, 34(11): 1447-1455.

GU Ying LI Chao LU Cui-Yun ZHENG Xian-Hu YU Ju-Hua SUN Xiao-Wen. Microsatellite markers for parentage identification in Jian Carp (Cyprinus carpio var. Jian)[J]. HEREDITAS, 2012, 34(11): 1447-1455.

参考文献

[1] 孙业良, 谢庄, 刘国庆, 任航行, 王刚, 代蓉. 利用微卫星DNA技术进行绵羊亲子鉴定. 安徽农业大学学报, 2005, 32(3): 301-305.
[2] 钱林东, 张自芳, 田应华, 袁峰, 钱坤, 苗永旺. 利用微卫星DNA标记进行黄牛的亲子鉴定. 云南农业大学学报, 2010, 25(1): 69-74.
[3] 刘秋玲, 吕德坚, 孙宏钰, 陆惠玲, 伍祥林, 伍新尧. 5个X-STR基因座荧光复合扩增体系的建立及法医学应用. 遗传, 2007, 29(12): 1459-1462.
[4] 李生斌, 阎春霞, 赖江华, 汪建, 杨焕明. DNA鉴定技术在法科学中的应用. 遗传, 2001, 23(2): 157-160.
[5] 高爱保, 吴登俊. 利用微卫星标记进行凉山半细毛羊亲权鉴定的研究. 遗传, 2005, 27(1): 85-90.
[6] 张建森, 孙小异. 建鲤新品系的选育. 水产学报, 2007, 31(3): 287-292.
[7] 董在杰, 朱健, 袁新华, 王建新. 建鲤基因组DNA的RAPD分析. 湛江海洋大学学报, 2002, 22(1): 3-6.
[8] Zhao JL, Cao Y, Li SF, Li JL, Deng YF, Lu GQ. Population genetic structure and evolutionary history of grass carp Ctenopharyngodon idella in the Yangtze River, China. Environ Biol Fish, 2011, 90(1): 85-93.
[9] Xu KF, Li Q, Kong LF, Yu RH. A first-generation genetic map of the Japanese scallop Patinopecten yessoensis-based AFLP and microsatellite markers. Aquacult Res, 2008, 40(1): 35-43.
[10] Sugaya T, Ikeda M, Mori H, Taniguchi N. Inheritance mode of microsatellite DNA markers and their use for kinship estimation in kuruma prawn Penaeus japoni-cus. Fisheries Sci, 2002, 68(2): 299-305.
[11] Barrous F, Gaudet J, Perrin N. Breeding system and ge-netic variance in the monogamous, semi-social shrew, Crocidura russula. Evolution, 1998, 52(4): 1230-1235.
[12] 鲁双庆, 刘臻, 刘红玉, 肖调义, 苏建明. 鲫鱼4群体基因组DNA遗传多样性及亲缘关系的微卫星分析. 中国水产科学, 2005, 12(4): 371-376.
[13] Sekino M, Sugaya T, Hara M, Taniguchi N. Relatedness inferred from microsatellite genotypes as a tool for brood-stock management of Japanese flounder Paralichthys olivaceus. Aquaculture, 2004, 233(1): 163-172.
[14] Perez-Enriquez R, Takagi M, Taniguchi N. Genetic vari-ability and pedigree tracing of a hatchery-reared stock of red sea bream (Pagrus major) used for stock enhancement, based on microsatellite DNA markers. Aquaculture, 1999, 173(1-4): 413-423.
[15] McDonald GJ, Danzmann RG, Ferguson MM. Relatedness determination in the absence of pedigree information in three cultured strains of rainbow trout (Oncorhynchus mykiss). Aquaculture, 2004, 233(1-4): 65-78.
[16] Jerry DR, Evans BS, MattKenway M, Wilson K. Development of a microsatellite DNA parentage marker suite for black tiger shrimp Penaeus monodon. Aquaculture, 2006, 255(1-4): 542-547.
[17] Castro J, Bouza C, Presa P, Pino QA, Riaza A, Ferreiro I, Sánchez L, Martínez P. Potential sources of error in par-entage assessment of turbot (Scophthalmus maximus) using microsatellite loci. Aquaculture, 2004, 242(1-4): 119-135.
[18] Boudry P, Collet B, Cornette F, Hervouet V, Bonhomme F. High variance in reproductive success of the Pacific oyster (Crassostrea gigas, Thunberg) revealed by microsatellite-based parentage analysis of multifactorial crosses. Aquaculture, 2002, 204(3-4): 283-296.
[19] Norris AT, Bradley DG, Cunningham EP. Parentage and relatedness determination in farmed Atlantic salmon (Salmo salar) using microsatellite markers. Aquaculture, 2000, 182(1-2): 73-83.
[20] Vandeputte M, Kocour M, Mauger S, Dupont-Nivet M, De Guerry D, Rodina M, Gela D, Vallod D, Chevassus B, Linhart O. Heritability estimates for growth-Related traits using microsatellite parentage assignment in juvenile common carp (Cyprinus carpio L.). Aquaculture, 2004, 235(1-4): 223-236.
[21] Vandeputte M, Rossignol MN, Pincent C. From theory to practice: Empirical evaluation of the assignment power of marker sets for pedigree analysis in fish breeding. Aquaculture, 2011, 314(1-4): 80-86.
[22] Zheng XH, Kuang YY, Zhang XF, Lu CY, Cao DC, Li C, Sun XW. A genetic linkage map and comparative genome analysis of common carp (Cyprinus carpio L.) us-ing microsatellites and SNPs. Mol Genet Genomics, 2011, 286(3-4): 261-277.
[23] 于飞, 王伟继, 孔杰, 阮晓红. 微卫星标记在大菱鲆(Scophthalmus maximus L.)家系系谱印证中的应用研究. 海洋学报, 2009, 31(3): 127-136.
[24] 王鸿霞, 吴长功, 张留所, 相建海. 微卫星标记应用于凡纳滨对虾家系鉴别的研究. 遗传, 2006, 28(2): 179-183.
[25] 纪志宾, 王桂芝, 王金凤, 陈珊珊, 王勇, 赵金山, 程明, 王建民. 崂山奶山羊分子系谱的构建及家系遗传特征分析. 中国农业科学, 2011, 44(13): 2796-2807.
[26] 董世瑞, 孔杰, 张天时, 孟宪红, 王如才. 中国对虾微卫星家系鉴定的模拟分析与应用. 水生生物学报, 2008, 32(1): 96-101.
[27] Pemberton JM, Slate J, Bancroft DR, Barrett JA. Nonam-plifying alleles at microsatellite loci: a caution for par-entage and population studies. Mol Ecol, 1995, 4(2): 249-252.
[28] Vigouroux Y, Jaqueth JS, Matsuoka Y, Smith OS, Beavis WD, Smith JS, Doebley J. Rate and pattern of mutation at microsatellite loci in maize. Mol Biol Evol, 2002, 19(8): 1251-1260.
[29] 程鹏, 杨爱国, 吴彪, 周丽青, 李霞. 微卫星标记在不同壳色虾夷扇贝家系亲权鉴定的适用性. 水生生物学报, 2011, 35(5): 768-775.
[30] Kline MC, Hill CR, Decker AE, Butler JM. STR sequence analysis for characterizing normal, variant, and null alleles. Genetics, 2011, 5(4): 329-332.
[31] Alderson GW, Gibbs HL, Sealy SG. Parentage and kinship studies in an obligate brood parasitic bird, the brown- headed cowbird (Molothrus ater), using microsa-tellite DNA markers. J Hered, 1999, 90(1): 182-190.
[32] Jones AG, Walker D, Avise JC, Stockwell CA. The molecular basis of a microsatellite null allele from the white sands pupfish. J Hered, 1998, 89(4): 339-342.
[33] 李琪. 皱纹盘鲍微卫星研究进展. 中国海洋大学学报(自然科学版), 2004, 34(3): 365-370.
[34] Bonin A, Bellemain E, Bronken Eidesen P, Pompanon F, Brochmann C, Taberlet P. How to track and assess geno-typing errors in population genetics studies. Mol Ecol, 2004, 13(11): 3261-3273.
[35] Hoffman JI, Amos W. Does kin selection influence fos-tering behaviour in Antarctic fur seals (Arctocephalus gazella)? Proc R Soc Lond Ser B: Biol Sci, 2005, 272(1576): 2017-2022.
[36] Castro J, Pino A, Hermida M, Bouza C, Riaza A, Ferreiro I, Sánchez L, Martínez P. A microsatellite marker tool for parentage analysis in Senegal sole (Solea senegalensis): Genotyping errors, null alleles and conformance to theoretical assumptions. Aquaculture, 2006, 261(4): 1194-1203.
[37] 黎中宝, 李少菁, 王桂忠. 锯缘青蟹(Scylla serrata)不同种群的杂合性研究. 海洋与湖沼, 2004, 35(4): 358-362.
[38] Ball AO, Leonard S, Chapman RW. Charaterization of (GT) sub(n) microsatellites from native white shrimp (Penaeus setiferus). Mol Ecol, 1998, 7(9): 1251-1253.

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建鲤(Cyprinus carpio var. Jian)微卫星DNA亲权鉴定

Microsatellite markers for parentage identification in Jian Carp (Cyprinus carpio var. Jian)

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