建鲤(Cyprinus carpio var. Jian)微卫星DNA亲权鉴定

doi:10.3724/SP.J.1005.2012.01447

遗传 ›› 2012, Vol. 34 ›› Issue (11): 1447-1455.doi: 10.3724/SP.J.1005.2012.01447

建鲤(Cyprinus carpio var. Jian)微卫星DNA亲权鉴定

顾颖^1, 李超¹, 鲁翠云¹, 郑先虎¹, 俞菊华², 孙效文¹

1. 中国水产科学研究院黑龙江水产研究所, 农业部淡水水产生物技术与遗传育种重点实验室, 哈尔滨 150070 2. 中国水产科学研究院淡水渔业研究中心, 无锡 214081

收稿日期:2012-03-28 修回日期:2012-05-12 出版日期:2012-11-20 发布日期:2012-11-25
通讯作者: 孙效文 E-mail:sunxw2002@163.com
基金资助:
公益性行业(农业)科研专项(编号：200903045), 国家重点基础研究发展计划项目(编号：2010CB126305)和农业部“引进国际先进农业科学技术”重点项目(编号：2011-G12) 资助

Microsatellite markers for parentage identification in Jian Carp (Cyprinus carpio var. Jian)

GU Ying¹, LI Chao¹, LU Cui-Yun¹, ZHENG Xian-Hu¹, YU Ju-Hua², SUN Xiao-Wen¹

1. Freshwater Fisheries Biotechnology and Genetic Breeding Key Lab of Ministry of Agriculture, Heilongjiang Fisheries Research Institute, Chinese Academy of Fisheries Sciences, Harbin 150070, China 2. Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, China

Received:2012-03-28 Revised:2012-05-12 Online:2012-11-20 Published:2012-11-25

摘要/Abstract

摘要： 利用16个微卫星座位对建鲤10个全同胞家系647个子代进行亲权鉴定。Cervus3.0分析表明, 16个微卫星位点的平均多态信息含量为0.7025, 平均等位基因数为6.63, 期望杂合度平均为0.7405。当双亲未知时, 累积排除概率为0.999225, 已知单亲时的累积排除概率为0.999996, 置信度为95%。进一步模拟分析表明, 要达到亲权鉴定的要求在双亲未知时通常需要8～12个微卫星位点, 已知单亲时需要5～8个微卫星位点。在双亲均未知的情况下进行亲权鉴定, 94.6%的后裔找到了其父母本, 真实鉴定率低于模拟分析预测值, 分析可能是与候选亲本间存在亲缘关系、无效等位基因的存在以及分型错误等因素有关。9个建鲤全同胞家系的鉴定, 为今后的遗传图谱构建、QTL定位及分子标记辅助育种研究奠定了基础。

关键词: 建鲤, 亲权鉴定, 微卫星

Abstract: Using 16 microsatellite loci we estimated the parentage of 647 progeny in 10 Jian Carp full-sib families. Cervus 3.0 analysis showed that mean PIC value of 16 microsatellites, mean number of allele, and mean expected heterozygosity were 0.7025, 6.63, and 0.7405, respectively. The combined probability of exclusion was 0.99922456 when both parents were unknown and the combined probability of exclusion was 0.99999557 when only one of the parental genotype was known, with the confidence level of 95%. Further simulations based on allele frequencies suggested that to achieve the requirements of paternity test usually took 8 to 12 microsatellite loci when both parents were unknown and 5 to 8 microsatellite loci when one parent was known. Out of 647 progenies, 94.6% were assigned to their parental pairs without the information of both parents in parentage analysis, which were lower than the theoretical assignment rates predicted by the Cervus simulations. This could be explained by the relationship between the candidate parents or existence of null and by typing errors. The identification of 9 families was useful for linkage analysis of Jian Carp and QTL location, also for marker assisted selection for economical traits.

Key words: Jian Carp, parental identification, microsatellite

顾颖，李超，鲁翠云，郑先虎，俞菊华，孙效文. 建鲤(Cyprinus carpio var. Jian)微卫星DNA亲权鉴定[J]. 遗传, 2012, 34(11): 1447-1455.

GU Ying LI Chao LU Cui-Yun ZHENG Xian-Hu YU Ju-Hua SUN Xiao-Wen. Microsatellite markers for parentage identification in Jian Carp (Cyprinus carpio var. Jian)[J]. HEREDITAS, 2012, 34(11): 1447-1455.

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建鲤(Cyprinus carpio var. Jian)微卫星DNA亲权鉴定

Microsatellite markers for parentage identification in Jian Carp (Cyprinus carpio var. Jian)

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