遗传 ›› 2014, Vol. 36 ›› Issue (10): 965-973.doi: 10.3724/SP.J.1005.2014.0965
钱秋杰,车家倩,叶露鹏,钟伯雄
收稿日期:2014-05-27
出版日期:2014-10-20
发布日期:2014-10-20
通讯作者:
钟伯雄,教授,博士生导师,研究方向：家蚕分子生物学。E-mail: bxzhong@zju.edu.cn
E-mail:qianqiujie08@163.com
作者简介:钱秋杰,硕士研究生,专业方向：转基因家蚕丝腺生物反应器。E-mail: qianqiujie08@163.com
基金资助:Qiujie Qian, Jiaqian Che, Lupeng Ye, Boxiong Zhong
Received:2014-05-27
Online:2014-10-20
Published:2014-10-20
摘要： piggyBac (PB)转座系统具有转座效率高、删除精确、半随机插入和携带片段较大等优点。但是作为一种转基因实验的工具,特别是在哺乳动物个体水平的转基因方面,还需要提高其转基因效率,并降低外源基因随机插入对内源基因破坏的风险。近年来的研究结果显示,PB转座系统得到了进一步改进：采用PB转座酶与DNA特异性结合蛋白融合而构成的融合型转座酶,表现出外源片段有插入到染色体靶向位点的倾向;采用突变体筛选的方法提高了PB转座酶的活性,获得了只具有切除活性而没有插入活性的新型PB转座酶;采用PB转座系统与细菌人工染色体(Bacterial artificial chromosomes, BAC)载体联合使携带的外源片段长度提高到了207 kb。改进后的PB转座系统在基因组研究、基因治疗、诱导多能干细胞(Induced pluripotent stem cells, iPSCs)诱导及其分化方面发挥了较大的作用。文章对PB转座系统的最新研究进展和应用前景进行了综述。
钱秋杰,车家倩,叶露鹏,钟伯雄. piggyBac转座系统的功能改进及在哺乳动物中的应用[J]. 遗传, 2014, 36(10): 965-973.
Qiujie Qian, Jiaqian Che, Lupeng Ye, Boxiong Zhong. The improvement and application of piggyBac transposon system in mammals[J]. HEREDITAS(Beijing), 2014, 36(10): 965-973.
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