遗传 ›› 1996, Vol. 18 ›› Issue (5): 36-38.

• 论文 • 上一篇    下一篇

组织凝集素在人染色体上的基因定位

吴秋茜; 蒋盘宏 WU Qiu-Qian;JIANG Pan-Hong   

  1. 北京化学试剂研究所生化试剂高技术实验室, 北京 100022 Biochemical Laboratory,Institute of Chemical Reagent,Beijing 100022
  • 收稿日期:1900-01-01 出版日期:1996-10-10 发布日期:1996-10-10

Localization of Sarcolectin Gene on Human Chromosome

  • Received:1900-01-01 Online:1996-10-10 Published:1996-10-10

摘要: 本文用非放射性DIG标记组织凝集素基因探针研究其在人染色体上的基因定
位,首先对基因原位杂交条件进行了一系列研究。结果表明采用染色体温和变性、基因
片段探针等为较好的基因原位杂交条件,通过135个细胞中期相的分析结果显示, 杂交阳
性率为20%,本底为2.81,杂交点分布峰位于3q12-13。
Abstract:The localization of sarcolectin gene on human chromosome is studied.A modified system of in situ hybridization was developed.A specific signal was generated by employing a nonradioactive DIG-labelled gene fragment as a probe in combination with the hybridization buffer containing no dextran sulphate and softly denatured chromosomes.Using this system,unique sequence for sarcolectin as small as 0.65kb was detectable and observed at 3q12-13.Of 135 metaphases examined,27(20%) had at least one grain deposited on 3q12-13,and the backgrand was 2.81.

关键词: 原位杂交
Key words,
Nonradioactive labeling, 人染色体, 组织凝集素, 非放射性标记, 部分同源染色体配对, 普通小麦, Ph1-like基因, Ae.cylindica

Key words: Common wheat Ae cylindrica Homoeologous pairing Backcross Ph1- like gene