摘要: 本文用非放射性DIG标记组织凝集素基因探针研究其在人染色体上的基因定
位,首先对基因原位杂交条件进行了一系列研究。结果表明采用染色体温和变性、基因
片段探针等为较好的基因原位杂交条件,通过135个细胞中期相的分析结果显示, 杂交阳
性率为20%,本底为2.81,杂交点分布峰位于3q12-13。
Abstract:The localization of sarcolectin gene on human chromosome is studied.A modified system of in situ hybridization was developed.A specific signal was generated by employing a nonradioactive DIG-labelled gene fragment as a probe in combination with the hybridization buffer containing no dextran sulphate and softly denatured chromosomes.Using this system,unique sequence for sarcolectin as small as 0.65kb was detectable and observed at 3q12-13.Of 135 metaphases examined,27(20%) had at least one grain deposited on 3q12-13,and the backgrand was 2.81.