遗传 ›› 1996, Vol. 18 ›› Issue (5): 39-42.

• 论文 • 上一篇    下一篇

制备绒毛细胞染色体的研究

辜士扬; 李蒙茹; 张秀玲 GU Shi-Yang;LI Meng-Ru;ZHANG Xiu-Ling   

  1. 天津医科大学总医院, 天津 300052 Tianjin Medical University Hospital,Tianjin 300052
  • 收稿日期:1900-01-01 出版日期:1996-10-10 发布日期:1996-10-10

A Study of Chromosome Preparation form Chorionic Villus

  • Received:1900-01-01 Online:1996-10-10 Published:1996-10-10

摘要: 在以往工作的基础上,建立了制备绒毛细胞染色体的24小时培养并结合酶解的方法。主要特点是将解离细胞、低渗处理与秋水仙素处理合并为一个步骤。用这种方法能使绒毛细胞染色体的制作成功率明显提高,并对研究内容进行了讨论。
Abstract:A study of enzymolysis for chromosome preparation from chorionic villus was established upon previous work .Essential feature of this technique was to merge cell separation from the cytotrophoblastic layer,treatment with hypotonic solution and colchicines treatment into one step.This technique significantly improved the success rate of chromosome analysis from CVS.The results of the study were discussed.

关键词: 孕早期诊断, 绒毛
Key words,
First trimester prenatal diagnosis, Chorionic villus, 配合力, 品系内选择, 复合育种值, 杂种优势, 约束选择

Key words: Compound breeding value Hybrid vigour Combining ability Restrained selection Inner-strain selection