遗传 ›› 2005, Vol. 27 ›› Issue (4): 575-578.

• 研究报告 • 上一篇    下一篇

凉山半细毛羊1号染色体微卫星遗传连锁图谱的构建

张明亚; 吴登俊   

  1. 四川农业大学动物科技学院,雅安 625014
  • 收稿日期:2004-05-21 修回日期:2005-02-20 出版日期:2005-08-10 发布日期:2005-08-10
  • 通讯作者: wudengjun

A Linkage Map of Mirosatellite on Chromosome 1 in Liangshan Semi-wool Sheep

ZHANG Ming-Ya, WU Deng-Jun   

  1. College of Animal Sci-Tech., Sichuan Agricultural University, Ya’an 625014, China
  • Received:2004-05-21 Revised:2005-02-20 Online:2005-08-10 Published:2005-08-10

摘要: 实验选择绵羊1号条染色体上的9个微卫星标记,采用父系半同胞家系群体(共387个个体)构建凉山半细毛羊1号染色体遗传连锁图。建立的资源参考家系通过20个微卫星标记进行了系谱确证。试验结果表明,9个标记的等位基因数变化范围为5~15个,杂合度在0.202~0.831之间,平均杂合度为0.617,各标记的平均多态信息含量PIC=0.604。构建的凉山半细毛羊1号条染色体遗传连锁图总长度311.0 cM,与美国肉畜中心(USDA)和国际绵羊作图中心(IMF)构建的绵羊1号条染色遗传连锁图结果基本一致。可用于下一步的QTL定位研究。

关键词: 微卫星标记, 凉山半细毛羊, 连锁图

Abstract: The 9 microsatellite markers on Chromosomes 1 were used to construct a linkage map of Liangshan semi-wool sheep with a 387 members half-sib pedigrees after paternity testing. The results were as follows: the number of alleles for 9 markers was from 5 to 15; the heterozygosity was from 0.202 to 0.831, the average heterozygosity was 0.617 and the polymorphic information content (PIC) was 0.604. Length of the linkage map of chromosome 1 we built was 311.0 cM which is in consistent with the sheep linkage map of USDA and IMF, and can be used in Quantitative Trait Loci searching in Liangshan semi-wool sheep.

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