番茄抗青枯病基因的AFLP分子标记

遗传 ›› 2006, Vol. 28 ›› Issue (2): 195-199.

番茄抗青枯病基因的AFLP分子标记

寿森炎¹; 冯壮志¹; 苗立祥¹;廖芳滨²

1.浙江大学园艺系蔬菜研究所生长发育与生物技术农业部重点实验室,杭州 310029；
2.浙江永隆山生物科技工程有限公司,诸暨 311800

收稿日期:2005-01-28 修回日期:2005-05-17 出版日期:2006-02-10 发布日期:2006-02-10
通讯作者: 冯壮志

Identification of AFLP Markers Linked to Bacterial Wilt Resistance Gene in Tomato

SHOU Sen-Yan,FENG Zhuang-Zhi ,MIAO Li-Xiang ,LIAO Fang-Bin

Department of Horticulture of Zhejiang University Growth and biotechnology of agricultural ministry key laboratory, Hangzhou 310029,China

Received:2005-01-28 Revised:2005-05-17 Online:2006-02-10 Published:2006-02-10
Contact: FENG Zhuang-Zhi

摘要/Abstract

摘要：

用番茄高抗青枯病品种“T51A”与高感青枯病品种“T9230”配制杂交组合,接种鉴定其正反交Ｆ₁代及Ｆ₂代分离群体的青枯病发生情况。结果表明,T51A对青枯病的抗性属于细胞质遗传,受1对杂合基因加性控制。用64个EcoRＩ/ＭseＩ引物组合对“T51A”、“T9230”两个亲本及其Ｆ₂代抗病和感病基因池进行AFLP分析,共扩增出约4200条可分辨的带,其中2条为稳定的差异。用“T51A”和“T9230”杂交产生的Ｆ₂代分离群体对2个特异条带与目的基因的遗传连锁性进行分析,发现特异条带AAG/CAT与暂定名为RRS-342的抗青枯病基因紧密连锁,二者之间的遗传距离为6.7 cM。将AAG/CAT片段回收、克隆和测序,成功地将其转化为SCAR标记,可以更加方便地用于对番茄青枯病基因的标记辅助选择。

关键词: 番茄, 青枯病, 抗性基因, 扩增片段长度多态性（AFLP）

Abstract: A cross between bacterial wilt resistant tomato variety “T51A”and susceptible variety ‘T9230’ was made for mapping bacterial wilt resistance gene(s).Through inoculation test of its F₁ and F₂ progeny, it was proved that the resistance of ‘T51A’ to bacterial wilt was controlled by one heterozygous gene and cytoplasm. With 64 EcoRＩ/ＭseＩprimer combinations, AFLP analysis was performed on two parents and their F₂ resistant and susceptible bulks. A total of about 4200 distinguishable bands were amplified, of which two were stable. Genetic linkage analysis of the two polymorphic DNA fragments with the resistance gene(s) was tested in the F₂ segregating population derived from the cross between ‘T51A’ and ‘T9230’. The DNA fragment AAG/ CAT was found closely linked to one of the bacterial wilt resistant genes, with a genetic distance of 6.7 cM, that was tentatively named RRS-342. The cloned fragment AAG/ CAT was sequenced and then successfully converted to a SCAR marker, which can be used more conveniently in marker- assisted selection for tomato resistance to bacterial wilt gene.

中图分类号:

Q943

寿森炎，冯壮志，苗立祥，廖芳滨. 番茄抗青枯病基因的AFLP分子标记[J]. 遗传, 2006, 28(2): 195-199.

SHOU Sen-Yan, FENG Zhuang-Zhi, MIAO Li-Xiang, LIAO Fang-Bin. Identification of AFLP Markers Linked to Bacterial Wilt Resistance Gene in Tomato[J]. HEREDITAS, 2006, 28(2): 195-199.

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