遗传 ›› 2009, Vol. 31 ›› Issue (7): 732-740.doi: 10.3724/SP.J.1005.2009.00732

• 研究报告 • 上一篇    下一篇

Grb10基因在小鼠胚期特异性表达分析

刘齐1;王燕2;陈岩1;张凤伟1;谷甜甜1;曲有鹏1;岳磊1;吴琼1   

  1. 1. 哈尔滨工业大学生命科学与工程系发育生物学研究室, 哈尔滨150001;
    2. 哈尔滨医科大学附属第一医院妇产科, 哈尔滨 150001
  • 收稿日期:2008-11-25 修回日期:2009-03-14 出版日期:2009-07-10 发布日期:2009-07-10
  • 通讯作者: 吴琼

The expression analysis of Grb10 during mouse embryonic development

LIU Qi1;WANG Yan2;CHEN Yan1;ZHANG Feng-Wei1;GU Tian-Tian1;QU You-Peng1;YUE Lei1;WU Qiong1
  

  1. 1. Developmental Biology Lab of Department of Life Science and Engineering, Harbin Institute of Technology, Harbin 150001, China;
    2. The Obstetrics and Gynecology Department of the First Hospital Affiliated to Harbin Medical University, Harbin 150001, China

  • Received:2008-11-25 Revised:2009-03-14 Online:2009-07-10 Published:2009-07-10
  • Contact: WU Qiong

摘要: 生长因子受体结合蛋白10(Growth factor receptor-bound protein 10, Grb10)是一个存在于小鼠11号染色体和人7号染色体的母本表达的印记基因。文章利用原位杂交技术和定量RT-PCR方法对不同发育阶段的小鼠胚胎Grb10 基因进行时空表达谱的分析, 以确定该基因在胚胎发育中其表达与组织发育的关系。定量RT-PCR数据结果表明, Grb10在E8.5-E13.5 (Embryonic days 8.5-13.5), 表达水平逐渐增高, 在E13.5达到高峰, 后期表达量回落。在胚胎发育的中后期脑、心、肺组织的表达水平呈递减趋势。肝脏组织中Grb10表达水平较为恒定, 在E18.5出现高峰。原位杂交数据验证了定量RT-PCR的结果, 并且说明了Grb10在其他如骨、肾和肌肉等组织器官的高表达水平。研究结果表明Grb10基因是小鼠胚期发育的一个重要的基因。

关键词: Grb10, 原位杂交, 定量RT-PCR, 小鼠

Abstract: Grb10 gene expression was analyzed in fetus and four organs during gestation process using the real-time RT-PCR and in situ hybridization. The real-time RT-PCR analysis result showed that the expression of Grb10 in whole em-bryos is throughout E8.5 to E19.5. The Grb10 gene expression level was gradually increased from E8.5 to E13.5, and then gradually reduced. The tissues specific expression levels were decreased in brain, heart, and lung from E12.5 to E19.5, but a peak expression was observed in liver at E18.5. Grb10 gene expression was also investigated at E13.5, E15.5, E16.5 and E18.5 sections by using in situ hybridization analysis. Strong signals of Grb10 were detected in brain, spine, kidney, and muscle tissues. These results suggest that Grb10 may play an important role during the development process in mouse.