遗传 ›› 2011, Vol. 33 ›› Issue (5): 469-484.doi: 10.3724/SP.J.1005.2011.00469

• 综述 • 上一篇    下一篇

条件基因打靶研究存在的主要问题及对策

林福玉, 杨晓   

  1. 军事医学科学院生物工程研究所发育和疾病遗传学研究室, 蛋白质组学国家重点实验室, 北京 100071
  • 收稿日期:2010-10-28 修回日期:2011-03-15 出版日期:2011-05-20 发布日期:2011-05-25
  • 通讯作者: 杨晓 E-mail:yangx@nic.bmi.ac.cn
  • 基金资助:

    蛋白质组学国家重点实验室深入研究课题(编号:SKLP-K200902), 国家科技重大专项新药创制(编号:2009ZX09501-027)资助

Issues and solutions of conditional gene targeting

LIN Fu-Yu, YANG Xiao   

  • Received:2010-10-28 Revised:2011-03-15 Online:2011-05-20 Published:2011-05-25
  • Contact: YANG Xiao E-mail:yangx@nic.bmi.ac.cn

关键词: 条件基因打靶, Cre-loxP, 位点特异性重组, 遗传背景

Abstract: Conditional gene targeting, based on the site-specific recombination system such as Cre-loxP, plays a vital role in the study of gene functions and the generation of disease mouse models. It was always under consideration that there were problems in the Cre-loxP recombination system, such as illegal expression pattern of Cre transgene, variation of Cre recombination efficiency and toxicity of Cre recombinase, as well as the potential influences of genetic background, breeding strategy, experimental control and gene compensation. Oversights of these issues may have a profound influence on the accuracy of gene functional dissection and conditional gene targeting mice phenotypic interpretation. Accordingly, solutions should be adopted including delicate regulative control of temporal-spatial specific Cre expression, detailed detection of Cre recombination efficiency, reduction of Cre toxicity, simplification of mouse genetic background, optimization of breeding, setting up of proper control and combined conditional gene targeting.

Key words: conditional gene targeting, Cre-LoxP, site-specific recombination, genetic background