干旱胁迫条件下马铃薯耐旱品种宁蒗182叶片蛋白质组学分析

doi:10.3724/SP.J.1005.2013.00666

遗传 ›› 2013, Vol. 35 ›› Issue (5): 666-672.doi: 10.3724/SP.J.1005.2013.00666

干旱胁迫条件下马铃薯耐旱品种宁蒗182叶片蛋白质组学分析

章玉婷^1,2,3, 周德群³, 苏源⁴, 余萍^1,2, 周晓罡^1,2, 姚春馨^1,2

1. 云南省农业科学院生物技术与种质资源研究所, 昆明 650223 2. 云南省农业生物技术重点实验室, 昆明650223 3. 昆明理工大学环境科学与工程学院, 昆明 650093 4. 昆明学院生命科学与技术系, 昆明 650214

收稿日期:2012-09-05 修回日期:2013-02-02 出版日期:2013-05-20 发布日期:2013-05-25
通讯作者: 周德群 E-mail:zxg88@163.com
基金资助:
云南省高层次人才引进工程(编号：2010C1120), 国家自然科学基金项目(编号：31060021)和云南省自然科学基金项目(编号：2006C0062M, 2008ZC100M)资助

Proteome analysis of potato drought resistance variety in Ninglang 182 leaves under drough stress

ZHANG Yu-Ting^1,2,3, ZHOU De-Qun³, SU Yuan⁴,YU Ping^1,2, ZHOU Xiao-Gang^1,2,YAO Chun-Xin^1,2

1. Biotechnology and Genetic Germplasm Institute, Yunnan Academy of Agricultural Sciences, Kunming 650223, China 2. Key Laboratory of Agricultural Biotechnology of Yunnan Province, Kunming 650223, China 3. Environmental Science and Engineering Faculty, Kunming University of Science and Technology, Kunming 650093, China 4. Life Science and Technology Faculty, Kunming University, Kunming 650214, China

Received:2012-09-05 Revised:2013-02-02 Online:2013-05-20 Published:2013-05-25

摘要/Abstract

摘要： 开展马铃薯抗旱分子机理的研究对培育马铃薯抗旱品种, 减少干旱造成的损失至关重要。文章利用双向电泳技术对云南地方耐旱马铃薯品种宁蒗182在干旱与正常处理条件下叶片表达差异蛋白质组进行对比研究。经电泳图谱分析和MALDI-TOF-TOF/MS质谱鉴定获得12个表达差异蛋白点, 并进行了功能分类。结果发现, 在差异蛋白中具有保护马铃薯光和系统以及线粒体正常运转的酶类; 调节该植株对环境胁迫响应的信号传导以及调控其组织内N、C运输系统的功能蛋白, 这些蛋白在受到干旱胁迫时表达量均升高。这一结果揭示出该类蛋白是马铃薯在干旱条件下产生的耐受相关蛋白。文章为阐释马铃薯抗旱品种通过多种路径和水平的调控提高其抗性的分子机理提供了理论依据。

关键词: 干旱胁迫, 双向电泳, 耐旱品种, 蛋白质组

Abstract: It is utmost important to cultivate potato variety with drought resistance to reduce arid loss by research progress on potato drought resistance molecular mechanism. A comparative study with differences in protein group analysis of potato drought resistance variety in Ninglang 182 leaves was carried out by using two-dimensional gel electrophoresis during drought and normal processing conditions.There were 12 differentially expressed protein spots identified by Electro- phoresis and MALDI-TOF-TOF/MS analysis. The function classification of these proteins results that the potato varieties to drought tolerance could be improved through the protection of photosynthesis and mitochondria, regulation of the signal transduction induced under environmental stress and regulation of plant tissue N and C transport system, these proteins expression were increased under drought.The results showed that these proteins are the drought resistance associated pro-teins of potato variety in Ninglang 182.This study provided a theoretical basis of the molecular mechanism of improving drought tolerance in order to expound the potato drought resistance variety through multiple paths and the level regulation.

Key words: drought resistance variety, proteomics, drought stress, two-dimensional gel electrophoresis

中图分类号:

S532
Q503

章玉婷周德群苏源余萍周晓罡姚春馨. 干旱胁迫条件下马铃薯耐旱品种宁蒗182叶片蛋白质组学分析[J]. 遗传, 2013, 35(5): 666-672.

ZHANG Yu-Ting ZHOU De-Qun SU Yuan YU Ping ZHOU Xiao-Gang YAO Chun-Xin. Proteome analysis of potato drought resistance variety in Ninglang 182 leaves under drough stress[J]. HEREDITAS, 2013, 35(5): 666-672.

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干旱胁迫条件下马铃薯耐旱品种宁蒗182叶片蛋白质组学分析

Proteome analysis of potato drought resistance variety in Ninglang 182 leaves under drough stress

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