甜瓜HDM基因家族鉴定及特性分析

doi:10.16288/j.yczz.23-226

遗传 ›› 2024, Vol. 46 ›› Issue (2): 168-180.doi: 10.16288/j.yczz.23-226

• 研究报告 • 上一篇

甜瓜HDM基因家族鉴定及特性分析

张治(), 张婧, 张瑾, 哈斯阿古拉, 郝金凤()

内蒙古大学生命科学学院，内蒙古自治区牧草与特色作物生物技术重点实验室，呼和浩特 010070

收稿日期:2023-08-23 修回日期:2023-09-25 出版日期:2024-02-20 发布日期:2023-10-25
通讯作者: 郝金凤 E-mail:1819296047@qq.com;haojindd@163.com
作者简介:张治，硕士研究生，专业方向：植物学。E-mail: 1819296047@qq.com
基金资助:
内蒙古自然科学基金项目(2020MS03025)

Identification and characterization analysis of the HDM gene family in melon (Cucumis melo L.)

Zhi Zhang(), Jing Zhang, Jin Zhang, Agula Hasi, Jinfeng Hao()

Inner Mongolia Key Laboratory of Herbage & Endemic Crop Biotechnology, School of Life Sciences, Inner Mongolia University, Hohhot 010070, China

Received:2023-08-23 Revised:2023-09-25 Published:2024-02-20 Online:2023-10-25
Contact: Jinfeng Hao E-mail:1819296047@qq.com;haojindd@163.com
Supported by:
Inner Mongolia Natural Science Foundation(2020MS03025)

摘要/Abstract

摘要：

组蛋白去甲基化酶(histone demethylase，HDM)是生物生长过程中的重要调节因子之一，在植物的生长发育和环境适应过程中发挥着重要的调控作用。本研究通过生物信息学方法对甜瓜(Cucumis melo L.) HDM基因家族进行了鉴定，并通过转录组数据检测了其成员在甜瓜组织的表达特性。结果显示，在甜瓜基因组中鉴定得到了20个CmHDM基因，在各条染色体上非均匀分布；其成员可以分成LSD1和JmjC两大类，而JmjC类群又可划分为5个亚群，各类群成员数量不等；种内和种间的共线性关系显示，甜瓜HDM基因仅存在一对片段重复，与番茄(Solanum lycopersicum) HDM基因共线性区域较多，亲缘关系较近；各成员所含保守结构域数量不等，且外显子和内含子在数量上有所差异；在启动子区域存在多种与激素和环境信号响应的顺式作用元件，表达特性分析显示各基因成员在甜瓜雄花、雌花、根、茎、叶、子房和成熟果实中均存在不同程度的表达。这些结果将有助于更好地理解HDM基因的潜在功能以及它们在参与调节甜瓜生长发育和环境自适中可能发挥的作用。

关键词: 甜瓜, HDM家族, 基因鉴定, 表达分析

Abstract:

Histone demethylase (HDM) play crucial roles in regulating plant growth and environmental adaptation. In this study, the HDM gene family in melon was identified by bioinformatics methods and the expression patterns of the CmHDM family members in different melon tissues were analyzed using transcriptome data. The results showed that 20 CmHDM genes were identified in the melon genome, which were unevenly distributed across each chromosome. These members fall into two major categories: LSD1 and JmjC. The JmjC group could be further divided into five subgroups with different numbers. The results of collinearity analysis of intraspecific and interspecific relationships showed that there were only one pair of segmental duplication in melon HDM genes, and more collinearity in genetic relationship of HDM genes between melon and tomato. The numbers of conserved domains, exons and introns in each member vary and various cis-acting elements responding to hormones and environmental signals existed in the respective promoter regions. Expression analysis showed that the respective gene members were expressed at different levels in male flowers, female flowers, roots, stems, leaves, ovary, and mature fruits of melon. These results will contribute to the understanding on the potential functions of the HDM genes and their potential functions in regulating melon growth and environmental adaptation.

Key words: melon, HDM family, gene identification, expression analysis

张治, 张婧, 张瑾, 哈斯阿古拉, 郝金凤. 甜瓜HDM基因家族鉴定及特性分析[J]. 遗传, 2024, 46(2): 168-180.

Zhi Zhang, Jing Zhang, Jin Zhang, Agula Hasi, Jinfeng Hao. Identification and characterization analysis of the HDM gene family in melon (Cucumis melo L.)[J]. Hereditas(Beijing), 2024, 46(2): 168-180.

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Identification and characterization analysis of the HDM gene family in melon (Cucumis melo L.)

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