遗传 ›› 2006, Vol. 28 ›› Issue (4): 422-426.

• 研究报告 • 上一篇    下一篇

10个牛品种线粒体12S rRNA基因多态性分析

孟彦1 2;许尚忠2 1;昝林森1;高雪2;陈金宝2;任红艳2;王兴平   

  1. 1. 西北农林科技大学动科学院,杨凌712100;2. 中国农业科学院畜牧研究所,北京100094
  • 收稿日期:2005-08-29 修回日期:2005-12-02 出版日期:2006-04-10 发布日期:2006-04-10
  • 通讯作者: 许尚忠

Analysis of Mitochondrial 12S rRNA Gene Polymorphism in Cattle

MENG Yan1 2,XU Shang-Zhong1 2,  ZAN Lin-Sen1,  GAO Xue2

CHEN Jin-Bao2,REN Hong-Yan2,WANG Xing-Ping   

  1. 1. Animal Sciences and Technology College, Northwest  A & F University,  Shaanxi 712100, China; 2. Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100094, China
  • Received:2005-08-29 Revised:2005-12-02 Online:2006-04-10 Published:2006-04-10
  • Contact: XU Shang-Zhong

摘要:

 
以鲁西牛、渤海黑牛、大别山牛、南阳牛、晋南牛、秦川牛、中国西门塔尔牛、日本和牛、海福特牛和安格斯牛10个牛品种共计353头牛为研究对象,利用单链构象多态性(SSCP)分子标记方法对其线粒体DNA 12S rRNA基因进行了多态性分析,结果发现该基因出现分布频率不同的3种单倍型(Ⅰ、Ⅱ、Ⅲ),其中单倍型Ⅰ出现的频率最低,仅在晋南牛1个个体中发现;单倍型Ⅱ在中国本地黄牛中都存在,在中国西门塔尔牛中的分布频率很低,但在3个国外品种海福特牛、安格斯牛和日本和牛中不存在;单倍型Ⅲ在这10个牛品种中均有分布,但以国外品种最丰富。计算平均多态信息含量,发现我国地方品种在0.232~0.423之间,基本属于中度多态,说明我国地方黄牛品种在12S rRNA上多态性比较丰富,国外品种较为贫乏;但是单倍型Ⅰ频率很低有丢失的趋势,需要对其进行保护。
 
 

关键词: 线粒体DNA, 牛, SSCP, 12S rRNA基因, 多态性

Abstract: The mitochondrial DNA 12S rRNA in seven Chinese local yellow cattle breeds, one cultivated breed and three imported breeds was studied by single-strand conformation polymorphism (SSCP). Three haplotypes (Ⅰ,Ⅱ, Ⅲ) were detected, with haplotypeⅠfound only in one cattle of the Jinnan breed. Haplotype Ⅱ did not appear in the foreign cattle breeds and low in Chinese-Simmental breed but was rich in Chinese local breeds. Haplotype Ⅲ had the widest distribution and was present in ten cattle breeds. The average polymorphism information content (PIC) for Chinese cattle between 0.232-0.423 was modest, which suggested relative abundance of genetic resources for the Chinese native cattle.


 

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