遗传 ›› 2010, Vol. 32 ›› Issue (6): 639-646.doi: 10.3724/SP.J.1005.2010.00639

• 研究报告 • 上一篇    

拟南芥在盐胁迫环境下SOS转录调控网络的构建及分析

谢崇波, 金谷雷, 徐海明, 朱军   

  1. 浙江大学农业与生物技术学院, 杭州310029
  • 收稿日期:2009-12-24 修回日期:2010-02-08 出版日期:2010-06-20 发布日期:2010-05-24
  • 通讯作者: 朱军 E-mail:jzhu@zju.edu.cn
  • 基金资助:

    农业部公益性行业科研专项(编号:200803034)和国家重点基础研究发展计划(973计划)项目(编号:2008CB117002)资助

Construction and analysis of SOS pathway-related transcriptional regulatory network underlying salt stress response in Arabidopsis

XIE Chong-Bo, JIN Gu-Lei, XU Hai-Ming, ZHU Jun   

  1. College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China
  • Received:2009-12-24 Revised:2010-02-08 Online:2010-06-20 Published:2010-05-24
  • Contact: SHU Jun E-mail:jzhu@zju.edu.cn

摘要:

研究拟南芥在高浓度盐处理环境下的基因调控网络, 有助于了解其在盐胁迫环境下保持正常生长的防御机制。针对目前广泛研究的SOS (Salt Overly Sensitive)耐盐机制, 文章整合公共数据库中盐胁迫相关的拟南芥基因组表达谱芯片, 通过反向工程方法构建了拟南芥在盐胁迫状态下的SOS转录调控网络。所获得的调控网络包含70个盐胁迫相关且高度互作的互作基因, 其中27个转录因子为主要调控节点。进而根据SOS核心基因的表达特性, 所得调控网络内的不同表达模式得到了鉴别。

关键词: 拟南芥, 盐胁迫, 转录调控网络, 转录因子, 基因表达

Abstract:

Elucidating gene regulatory network of Arabidopsis thaliana under high salt treatment is crucial to understand the defense mechanism of maintaining normal growth rate. Here, an Arabidopsis Salt Overly Sensitive (SOS) transcriptional regulatory network under salinity stress was constructed using a reverse engineering method on published genome-wide expression profiles. In this study, the SOS regulatory network constructed contains 70 genes, of which 27 are highly interconnected transcription factors. According to the expression feature of key genes in SOS signaling pathway, distinct expression patterns of the regulatory network were identified.

Key words: Arabidopsis thaliana, salt stress, transcriptional regulatory network, transcription factors, gene expression