植物质体基因工程调控元件研究进展

doi:10.16288/j.yczz.23-021

遗传 ›› 2023, Vol. 45 ›› Issue (6): 501-513.doi: 10.16288/j.yczz.23-021

植物质体基因工程调控元件研究进展

于一凡^1,²(), 欧阳臻², 郭娟¹, 赵瑜君¹(), 黄璐琦^1,²()

1.中国中医科学院中药资源中心，科技部与国家中医药管理局道地药材国家重点实验室，北京 100700
2.江苏大学食品与生物工程学院，镇江 212013

收稿日期:2023-01-30 修回日期:2023-04-29 出版日期:2023-06-20 发布日期:2023-05-22
通讯作者: 赵瑜君,黄璐琦 E-mail:18641603721@163.com;zhaoyj@nrc.ac.cn;huangluqi01@126.com
作者简介:于一凡，在读博士研究生，专业方向：分子生药学。E-mail: 18641603721@163.com
基金资助:
中央本级重大增减支项目“名贵中药资源可持续利用能力建设”(2060302);中央级公益性科研院所基本科研业务费专项资金(ZZ15-YQ-061);中央级公益性科研院所基本科研业务费专项资金(ZZXT202103)

Progress on regulatory elements of plant plastid genetic engineering

Yifan Yu^1,²(), Zhen OuYang², Juan Guo¹, Yujun Zhao¹(), Luqi Huang^1,²()

1. State Key Laboratory of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China
2. School of Food and Biological Engineering, Jiangsu University, Zhenjiang 212013, China

Received:2023-01-30 Revised:2023-04-29 Online:2023-06-20 Published:2023-05-22
Contact: Zhao Yujun,Huang Luqi E-mail:18641603721@163.com;zhaoyj@nrc.ac.cn;huangluqi01@126.com
Supported by:
Key Project at Central Government Level(2060302);Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZ15-YQ-061);Fundamental Research Funds for the Central Public Welfare Research Institutes(ZZXT202103)

摘要/Abstract

摘要：

随着植物合成生物学的发展，质体逐渐成为许多具有商业价值的次生代谢产物和治疗性蛋白异源生产的理想平台。与核基因工程相比，质体基因工程在外源基因高效表达和生物安全性等方面具有其独特优势。然而，外源基因在质体系统中的组成型表达或对植物生长不利，因此需进一步挖掘、设计调控元件实现对外源基因的精准调控。本文概述了质体基因工程调控元件的研究进展，内容包括操纵子设计与优化思路、多基因共表达调控策略及新型表达调控元件的挖掘等，为植物合成生物学的发展提供参考。

关键词: 植物合成生物学, 质体基因工程, 遗传转化, 操纵子, 调控元件

Abstract:

With the advancement of plant synthetic biology, plastids have emerged as an optimal platform for the heterologous production of numerous commercially valuable secondary metabolites and therapeutic proteins. In comparison on nuclear genetic engineering, plastid genetic engineering offers unique advantages in terms of efficient expression of foreign genes and biological safety. However, the constitutive expression of foreign genes in the plastid system may impede plant growth. Therefore, it is imperative to further elucidate and design regulatory elements that can achieve precise regulation of foreign genes. In this review, we summarize the progress made in developing regulatory elements for plastid genetic engineering, including operon design and optimization, multi-gene coexpression regulation strategies, and identification of new expression regulatory elements. These findings provide valuable insights for future research.

Key words: plant synthetic biology, plastid genetic engineering, genetic transformation, operon, regulatory element

于一凡, 欧阳臻, 郭娟, 赵瑜君, 黄璐琦. 植物质体基因工程调控元件研究进展[J]. 遗传, 2023, 45(6): 501-513.

Yifan Yu, Zhen OuYang, Juan Guo, Yujun Zhao, Luqi Huang. Progress on regulatory elements of plant plastid genetic engineering[J]. Hereditas(Beijing), 2023, 45(6): 501-513.

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研究内容	启动子	5′UTR	3′UTR	参考文献
苦瓜(Momordica charantia)叶绿体转化载体的构建及其功能评价	PpsbA	psbA	TpsbA	[41]
可育转质体拟南芥植物的高效培育	Prrn/PpsbA/Pclp	rbcL/T7g10L	TpsbA/TatpA/Trps16/ TatpB/TrrnB	[19]
适用于拟南芥质体转化的载体及壮观霉素敏感性拟南芥品系的开发	Prrn	atpB	TpsbA	[42]
莴苣叶绿体用于生产口服增强型疫苗抗原SARS-CoV-2刺突蛋白	Prrn/PpsbA	psbA	TrbcL/TpsbA	[43]
RuBisCO的基因工程改良	PrbcL	rbcL	Trps16/TrbcL	[31]
药用植物黄花蒿叶绿体遗传转化体系的建立	Prrn/PpsbA	T7g10L/psbA	TpsbA/Trps16	[24]
揭示质体编码的乙酰辅酶A羧化酶基因对代谢和发育功能的影响	Prrn		TpsbA	[44]
将复制微型染色体开发为质体基因工程的新工具	Prrn/PclpP	T7g10L	TrrnB/TpsbA	[45]

研究内容	启动子	5′UTR	3′UTR	参考文献
烟草新型IEE的开发	Prrn	T7g10L	TpsbA/TrbcL/Trps16	[92]
莱茵衣藻新型IEE的开发	PrbcL	T7g10L	TrbcL	[38]
工程化PPR蛋白在新型质体诱导表达调控系统中的应用	Prrn/PpsbA	atpB/atpH	TpsbA/TrbcL	[71]
工程化RNA结合蛋白在新型非绿色质体诱导表达调控系统中的应用	Prrn	atpB/atpH	TpsbA/TrbcL	[99]
质体未加工多顺反子中ORF独立翻译合成系统的开发	Prrn/PpsbA	clp/rbcL/atpB/T7g10L/ psbA/cry9Aa2/atpH	TpsbA	[106]
核编码翻译增强子TDA1在莱茵衣藻叶绿体诱导表达调控系统中的应用	Prrn	atpA		[72]
核糖开关质体诱导表达系统在虾青素生物合成中的应用	T7/Prrn/PpsbA	T7g10L/psbA	TpsbA/TatpA/Trps16/ TrbcL/T7 T	[33]
莱茵衣藻新型温度敏感表达调控系统的开发	PpsaA	psaA	TrbcL	[105]
莱茵衣藻维生素可逆性调控系统的开发	PpsbD/PpsaA	psbD/psaA	TrbcL/TpsaD	[107]

植物质体基因工程调控元件研究进展

Progress on regulatory elements of plant plastid genetic engineering

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