遗传 ›› 2015, Vol. 37 ›› Issue (10): 992-291.doi: 10.16288/j.yczz.15-291
李金环, 寿佳, 吴强
收稿日期:2015-06-23
出版日期:2015-10-20
发布日期:2015-10-20
通讯作者:
吴强,博士,教授,博士生导师,研究方向：基因表达调控及神经发育。E-mail: qwu123@gmail.com
作者简介:李金环,博士研究生,专业方向：遗传学。 E-mail: lijinhuan163@126.com。寿佳,博士研究生,专业方向：遗传学。 E-mail: shoujia1106@163.com。李金环和寿佳同为第一作者
基金资助:Jinhuan Li， Jia Shou， Qiang Wu
Received:2015-06-23
Online:2015-10-20
Published:2015-10-20
摘要： 源于细菌和古菌的Ⅱ型成簇规律间隔短回文重复系统[Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated nuclease 9 (Cas9),CRISPR/Cas9]近年被改造成为基因组定点编辑的新技术。由于它具有设计简单、操作方便、费用低廉等巨大优势,给遗传操作领域带来了一场革命性的改变。本文重点介绍了CRISPR/Cas9系统在基因组DNA片段靶向编辑方面的研究和应用,主要包括DNA片段的删除、反转、重复、插入和易位,这一有效的DNA片段编辑方法为研究基因功能、调控元件、组织发育和疾病发生发展提供了有力手段。本文最后展望了Ⅱ型CRISPR/Cas9系统的应用前景和其他类型CRISPR系统的应用潜力,为开展利用基因组DNA片段靶向编辑进行基因调控和功能研究提供参考。
李金环, 寿佳, 吴强. CRISPR/Cas9系统在基因组DNA片段编辑中的应用[J]. 遗传, 2015, 37(10): 992-291.
Jinhuan Li， Jia Shou， Qiang Wu. DNA fragment editing of genomes by CRISPR/Cas9[J]. HEREDITAS(Beijing), 2015, 37(10): 992-291.
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