遗传 ›› 2002, Vol. 24 ›› Issue (3): 335-336.

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简单快速的DNA银染和胶保存方法

许绍斌;陶玉芬;杨昭庆;褚嘉祐 XU Shao-bin;TAO Yu-fen;YANG Zhao-qing;CHU Jia-you   

  1. 中国医学科学院 中国协和医科大学医学生物学研究所遗传室,昆明650118 Department of Genetics,Institute of Medical Biology,Chinese Academy of Medical Sciences& Peking Union Medical College,Kunming 650118,China
  • 收稿日期:1900-01-01 出版日期:2002-06-10 发布日期:2002-06-10

A Simple and Rapid Methods Used for Silver Staining and Gel Preservation

  • Received:1900-01-01 Online:2002-06-10 Published:2002-06-10

摘要: 本文介绍了一套简单快速的DNA银染以及胶保存的方法,整个过程仅需10~15分钟,而且背景浅,条带清楚,灵敏度高,稳定性好。胶保存采用双层玻璃纸夹心法,可长久地保存胶显色时的原貌。以常规PAG胶检测和HLA的SSCP分型为例,利用该套方法进行了银染以及胶的保存,均得到了满意的结果。该方法具有推广价值。
Abstract:This paper introduced the simple and rapid methods of silver staining and gel preservation.It was taken only about 10 and 15 minutes to stain a gel.The background of gel was light,the bands were clear,the sensibility was high and the stabilization was well by the method of silver staining.The gel preservation adopted a method named two-layer transparent plastic paper "Sandwich" which could keep the gel with primitive colors for a long time.The methods were used on PAG checking and SSCP typing of HLA and the results were satisfactory.The set of methods are expected to be widely used in laboratories.

关键词: 银染;胶保存;PAG;SSCP
Key words;silver staining,
gel preservation, PAG, SSCP