郏县红牛ZAG基因多态性与生长性状的相关性

doi:10.3724/SP.J.1005.2008.01417

遗传 ›› 2008, Vol. 30 ›› Issue (11): 1417-1420.doi: 10.3724/SP.J.1005.2008.01417

郏县红牛ZAG基因多态性与生长性状的相关性

郭义昆¹;陈宏^{1, 2};张宝¹;潘传英¹;张良志¹;赵苗¹;张存芳¹;蓝贤勇¹;王居强³

1. 西北农林科技大学动物科技学院, 陕西省农业分子生物学重点实验室, 杨凌 712100;
2. 徐州师范大学细胞与分子生物学研究所, 徐州 221116;
3. 河南肉牛工程技术研究中心, 郑州 450003

收稿日期:2008-04-08 修回日期:2008-07-18 出版日期:2008-11-10 发布日期:2008-11-10
通讯作者: 陈宏

Polymorphisms of ZAG gene with growth traits in Jiaxian red cattle

GUO Yi-Kun¹;CHEN Hong^{1, 2};ZHANG Bao¹;PAN Chuan-Ying¹;ZHANG Liang-Zhi¹;ZHAO Miao¹;ZHANG Cun-Fang¹;LAN Xian-Yong¹;WANG Ju-Qiang³

1. College of Animal Science and Technology, Northwest A& F University, Shaanxi Key Laboratory of Molecular Biology for Agriculture, Yangling, Shaanxi 712100, China;
2. Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou 221116, China;
3. The Research Center of Cattle Engineering Technology in Henan, Zhengzhou 450003, China

Received:2008-04-08 Revised:2008-07-18 Online:2008-11-10 Published:2008-11-10
Contact: CHEN Hong

摘要/Abstract

摘要： 摘要: ZAG基因的功能主要是促进脂肪分解, 减少脂肪含量。文章利用PCR-SSCP和DNA测序技术研究了145头郏县红牛ZAG基因编码区4个位点(Z1、Z2、Z3、Z4)的多态性, 发现Z1、Z3、Z4 位点存在SSCP多态。对不同SSCP带型的对应片段进行了测序分析, 共发现6个新的SNP多态位点(C115T、A3257G、A4013G、T4027C、C4032T、T4120C)。Z3位点处于Hardy-Weinberg平衡状态, Z1、Z4 位点处于非平衡状态。不同基因型与生长发育性状的相关性分析显示, Z4位点上, AC基因型个体的体斜长、胸围、管围、体重指标显著(P<0.05)或极显著(P<0.01), 大于AA、AB基因型个体, 暗示该位点有可能作为郏县红牛生长性状标记辅助选择的标记之一。

关键词: 郏县红牛, ZAG基因, PCR-SSCP, 生长发育性状

Abstract:

Abstract: The main function of ZAG gene is to enable the decomposition of fat, and reduced fat content. In this study, polymorphisms of four loci (Z1, Z2, Z3, Z4) at the coding region of the bovine ZAG gene were detected in 145 Jiaxian red cattle, and polymorphisms were found on Z1, Z3, Z4 loci. The fragments showing different SSCP patterns were sequenced, nd a total of six SNPs (C115T, A3257G, A4013G, T4027C, C4032T, and T4120C) were found. The Z3 locus of ZAG gene was at Hardy-Weinberg equilibrium, while Z1 and Z4 loci of ZAG gene were not at Hardy-Weinberg disequilibrium. The association analysis of different genotypes with some of the growth and development traits showed that the individuals with genotype AC had a larger body length, heart girth, circumference of cannon bone, body weight than the individuals with genotypes AA and AB (P<0.05 or P<0.01, respectively) in Z4 locus. It is suggest that Z4 locus may be one of the marker-assisted selection used as growth traits in Jiaxian red cattle.

郭义昆，陈宏，张宝，潘传英，张良志，赵苗，张存芳，蓝贤勇，王居强. 郏县红牛ZAG基因多态性与生长性状的相关性[J]. 遗传, 2008, 30(11): 1417-1420.

GUO Yi-Kun, CHEN Hong, ZHANG Bao, PAN Chuan-Ying, ZHANG Liang-Zhi, ZHAO Miao, ZHANG Cun-Fang, LAN Xian-Yong, WANG Ju-Qiang. Polymorphisms of ZAG gene with growth traits in Jiaxian red cattle[J]. HEREDITAS, 2008, 30(11): 1417-1420.

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郏县红牛ZAG基因多态性与生长性状的相关性

Polymorphisms of ZAG gene with growth traits in Jiaxian red cattle

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