遗传 ›› 2007, Vol. 29 ›› Issue (9): 1089-1089―1096.doi: 10.1360/yc-007-1089

• 研究报告 • 上一篇    下一篇

MyoG基因多态性与优质肉鸡屠宰性状和肉质性状的相关性分析

王琼1; 刘益平1; 蒋小松2; 杨朝武1; 杜华瑞2; 邱莫寒1; 朱庆1   

  1. 1. 四川农业大学动物科技学院, 雅安 625014;
    2. 四川省畜牧科学研究院, 成都 610066

  • 收稿日期:2006-12-01 修回日期:2007-04-05 出版日期:2007-09-10 发布日期:2007-09-10
  • 通讯作者: 朱庆

Correlation analysis of relationships between polymorphisms of high quality chicken myogenin gene and slaughter and meat quality traits

WANG Qiong1; LIU Yi-Ping1; JIANG Xiao-Song2; YANG Chao-Wu1; DU Hua-Rui2;
QIU Mo-Han1; ZHU Qing1
  

  1. 1. Animal Science and Technology College of Sichuan Agriculture University, Ya’an 625014, China;
    2. Sichuan Province Institute of Animal Husbandry Research, Chengdu 610066, China
  • Received:2006-12-01 Revised:2007-04-05 Online:2007-09-10 Published:2007-09-10
  • Contact: ZHU Qing

摘要:

采用PCR-SSCP分析方法, 对8个品系240只个体的大恒优质肉鸡进行了MyoG基因5′调控区的研究。结果表明, MyoG基因5′调控区存在A、B 2个多态位点, 结合测定的生产资料, 分析了该基因5′调控区两个变异位点产生的基因型之间在屠宰性状和肉质性状的差异并进行了最小二乘分析和显著性检验。表明MyoG基因对鸡的肌纤维生长发育有显著的正相关。

关键词: 屠体性状, PCR-SSCP, 肉质性状, MyoG基因,

Abstract:

PCR-SSCP technique was designed in this study to investigate the effect of MyoG on quality meat chicken (developed by Sichuan Dahen Poultry Breeding Company using local breeds). Four mutations at base position in promoter were detected among individuals in each line, i.e. T/C in locusA and T/A, T/C and A/G in locus B . The least square analysis showed that there were a significant difference between genotypes and breast muscle percentage and some carcass traits (P
<0.05)for locus A. There were a significant difference (P <0.05) in breast muscle weight between AC、AA and AB geno-
types; a significant difference (P <0.05) in leg muscle percentage between CC and AC for locus B, and a extremity signifi-cant difference (P <0.01) in the frequency of genotype muscle Fibre Density for Bothlocus A to locus B. There was no sig-nificant difference (P >0.05) in the other triats. It was concluded from the results that MyoG gene is the major gene affect-ing the muscle fiber traits of chicken or it links with the candidate gene, and the mutation could be used as the molecular genetic marker to select the chickens for Meat Quality traits.