组蛋白去乙酰化酶抑制剂影响的代谢相关基因的组学筛查及验证

doi:10.16288/j.yczz.15-128

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HEREDITAS(Beijing) ›› 2015, Vol. 37 ›› Issue (9): 918-925.doi: 10.16288/j.yczz.15-128

• Research Articles • Previous Articles Next Articles

Transcriptome screening and verification of genes related to metabolism affected by histone deacetylase inhibitors

Jihong Cao¹, Weiting Liao², Cheng Wo¹, Guorong Xu¹, Huanxin Xu¹, Pinglong Li¹, Ye Tao¹, Peng Wang¹, Jiari Lin², Lianrui Deng³

1. Jiujiang Third People's Hospital, Jiujiang 332000, China;
2. Institute for Translational Medicine, Nanchang University, Nanchang 330000, China;
3. The Second Affiliated Hospital, Nanchang University, Nanchang 330000, China

Received:2015-05-12 Revised:2015-08-09 Online:2015-09-20 Published:2015-09-20

Abstract

Abstract: Histone deacetylases (HDACs) are responsible for catalyzing the deacetylation of histones, which closely related to many biological processes such as cell proliferation, differentiation and apoptosis. In recent years, HDAC inhibitors (HADCIs), with the anti-tumor potential, have been hot-spots of drug screening. Although the latest studies suggested that HDAC2 might influence the metabolism, the mechanism of HDACIs in metabolic regulation is still unclear. Here, we integrated the gene expression profiling of HDACIs (TSA and SAHA) in hepatocellular carcinoma cell (HepG2). The results showed 380 differentially expressed genes (DEGs) and 35 KEGG pathways enriched by DEGs in TSA-treatment group. Most of DEGs (177/380) and KEGG pathways (23/35) from TSA-treatment groups were confirmed by SAHA-treatment. About half of KEGG pathways (9/23) were related to metabolism ,and nearly one third of common DEGs (66/177) were involved in metabolic process. Moreover, HDAC2 siRNA experiment verified the effect of HDACIs on metabolic genes, suggesting that HDACIs potentially present a practical value to prevent tumor and other metabolism-related diseases.

Key words: HDACIs, metabolism, gene expression

Jihong Cao, Weiting Liao, Cheng Wo, Guorong Xu, Huanxin Xu, Pinglong Li, Ye Tao, Peng Wang, Jiari Lin, Lianrui Deng. Transcriptome screening and verification of genes related to metabolism affected by histone deacetylase inhibitors[J]. HEREDITAS(Beijing), 2015, 37(9): 918-925.

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Transcriptome screening and verification of genes related to metabolism affected by histone deacetylase inhibitors

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