过表达组蛋白H3K9me3去甲基化酶对猪克隆胚胎发育的影响

doi:10.16288/j.yczz.22-244

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Hereditas(Beijing) ›› 2023, Vol. 45 ›› Issue (1): 67-77.doi: 10.16288/j.yczz.22-244

• Research Article • Previous Articles Next Articles

Effects of overexpression of histone H3K9me3 demethylase on development of porcine cloned embryos

Yanan Li^1,^2,³(), Xianjun Zhang^1,^2,³, Ning Zhang^1,^2,³, Yalin Liang^1,^2,³, Yuxing Zhang^1,^2,³, Huaxing Zhao^1,^2,³, Zicong Li^1,^2,³, Sixiu Huang^1,^2,³()

1. National Engineering Research Center for Breeding Swine Industry, College of Animal Science of South China Agricultural University, Guangzhou 510642, China
2. National & Local Joint Engineering Research Center for Breeding Animal Industry, Guangzhou 510642, China
3. Guangdong Provincial Key Laboratory of Agro-animal Genomics and Molecular Breeding, Guangzhou 510642, China

Received:2022-07-18 Revised:2022-11-08 Online:2023-01-20 Published:2022-11-28
Contact: Huang Sixiu E-mail:li321736803@163.com;sxhuang815@scau.edu.cn
Supported by:
the Natural Science Foundation of Guangdong Province(2019B1515210027);Guangdong Special Support Plan Local Innovation and Entrepreneurship Team(2019BT02N630);Guangdong Province Livestock and poultry Local Breed Protection and Development and Utilization Promotion Project]

Abstract

Abstract:

The abnormal modification of histone is an important factor restricting development of porcine cloned embryos. Overexpression of histone H3K9me3 demethylase KDM4 family can effectively improve the developmental efficiency of cloned embryos. In order to explore the effects of overexpression of H3K9me3 demethylase on the development of porcine cloned embryos, KDM4A mRNA and KDM4D mRNA were injected respectively into porcine cloned embryos at the 1-cell stage and 2-cell stage to detect the blastocyst rate; 2-cell stage cloned embryos injected with KDM4A mRNA and embryo injection water (the control group) at the 1-cell stage were collected to detect the expression level of H3K9me3, and 4-cell stage cloned embryos were collected for single cell transcriptome sequencing, then the sequencing data was analyzed with KEGG and GO. The results showed that the blastocyst rate of porcine cloned embryos injected with KDM4A mRNA at 1-cell stage was significantly higher than that of the control group (25.32 ± 0.74% vs 14.78 ± 0.87%), while cloned embryos injected with KDM4D mRNA had a similar blastocyst rate with cloned embryos in control group (16.27 ± 0.77% vs 14.78 ± 0.87%). Porcine cloned embryos injected with KDM4A mRNA and KDM4D mRNA at 2-cell stage had a similar blastocyst rate with cloned embryos in control group (32.18 ± 1.67%, 30.04 ± 0.91% vs 31.22 ± 1.40%). The expression level of H3K9me3 in cloned embryos injected with KDM4A mRNA at 1-cell stage was lower than that in control group. There were 133 differentially expressed genes detected by transcriptome sequencing, including 52 up-regulated genes and 81 down-regulated genes. Pathways enriched by GO analyses were mainly related to protein localization. Pathways enriched by KEGG analyses were related to cellular senescence and acute myeloid leukemia. These results suggest that overexpression of histone H3K9me3 demethylase KDM4A can significantly improve the developmental efficiency of porcine cloned embryos.

Key words: pig, cloning embryo, H3K9me3, KDM4A, KDM4D

Yanan Li, Xianjun Zhang, Ning Zhang, Yalin Liang, Yuxing Zhang, Huaxing Zhao, Zicong Li, Sixiu Huang. Effects of overexpression of histone H3K9me3 demethylase on development of porcine cloned embryos[J]. Hereditas(Beijing), 2023, 45(1): 67-77.

Figures/Tables 6

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组别	胚胎数(n)	卵裂数	卵裂率(%)	囊胚数	囊胚率(%)	囊胚细胞数
KDM4A	308(8)	228	74.03 ± 0.95	78	25.32 ± 0.74^aA	42.11 ± 2.39
KDM4D	295(8)	217	73.45 ± 0.92	48	16.27 ± 0.77^bB	43.79 ± 2.70
KDM4A+KDM4D	216(7)	155	71.76 ± 0.93	41	18.98 ± 1.01^abAB	38.59 ± 2.8
胚胎注射水(对照)	345(9)	250	72.46 ± 0.65	51	14.78 ± 0.87^bB	40.27 ± 2.22

组别	胚胎数(n)	囊胚数	囊胚率(%)	囊胚细胞数
KDM4A	202(6)	65	32.18 ± 1.67	35.18 ± 1.73^ab
KDM4D	243(7)	73	30.04 ± 0.91	32.47 ± 1.75^b
KDM4A+KDM4D	88(3)	26	29.55 ± 1.95	40.33 ± 4.00^a
胚胎注射水(对照)	221(6)	69	31.22 ± 1.40	35.44 ± 2.05^ab

Effects of overexpression of histone H3K9me3 demethylase on development of porcine cloned embryos

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