利用转录组测序筛选鸡蛋褐壳性状相关基因

doi:10.16288/j.yczz.17-111

遗传 ›› 2017, Vol. 39 ›› Issue (11): 1102-1111.doi: 10.16288/j.yczz.17-111

利用转录组测序筛选鸡蛋褐壳性状相关基因

李光奇^1,²(),孙从佼¹(),吴桂琴²,石凤英²,刘爱巧²,孙皓²,杨宁¹()

1. 中国农业大学动物科技学院,北京100193
2. 北京市华都峪口禽业有限责任公司,北京101206

收稿日期:2017-03-28 修回日期:2017-09-02 出版日期:2017-11-20 发布日期:2017-12-20
作者简介:李光奇,博士,博士后,研究方向：家禽分子遗传育种。E-mail: lgq717535@126.com|孙从佼,博士,讲师,研究方向：家禽分子遗传育种。E-mail: cjsun@cau.edu.cn|杨宁，博士，教授，研究方向：家禽分子遗传育种。E-mail: nyang@cau.edu.cn
基金资助:
国家自然科学基金项目(31501915);国家蛋鸡产业技术体系项目(2017QC009, 2017DK001);国家蛋鸡产业技术体系项目(CARS-41);长江学者和创新团队发展计划项目(IRT119)

Transcriptome sequencing identifies potential regulatory genes involved in chicken eggshell brownness

Li Guangqi^1,²(),Sun Congjiao¹(),Wu Guiqin²,Shi Fengying²,Liu Aiqiao²,Sun Hao²,Yang Ning¹()

1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
2. Beijing Huadu Yukou Poultry Industry Co. Ltd., Beijing 101206, China

Received:2017-03-28 Revised:2017-09-02 Online:2017-11-20 Published:2017-12-20
Supported by:
the National Natural Science Foundation of China(31501915);Chinese Universities Scientific Fund(2017QC009, 2017DK001);National Agricultural Technology System (Layer)(CARS-41);Programs for Changjiang Scholars and Innovative Research in University(IRT119)

摘要/Abstract

摘要：

褐壳鸡蛋在许多国家深受消费者喜爱,消费者通常把蛋壳褐色深浅作为评价鸡蛋品质的重要指标。褐壳鸡蛋蛋壳颜色形成受多基因共同调控,但是具体候选基因及调控机理尚未明确。因此,本研究以纯系褐壳蛋鸡为实验材料,筛选调控褐壳鸡蛋颜色深浅的候选基因。采用转录组测序技术对产深褐壳鸡蛋和浅褐壳鸡蛋的母鸡蛋壳腺组织进行分析,结果显示,共计有8461个基因在蛋壳腺组织表达,其中34个基因在两组之间差异表达。功能分析发现,卵转铁蛋白(ovotransferrin, TF)基因、热休克蛋白70 (heat shock protein, HSP70)基因以及氧化磷酸化通路均与原卟啉Ⅸ合成通路相关,可能影响褐壳鸡蛋蛋壳色素原卟啉Ⅸ的合成和积累。

关键词: 鸡蛋, 褐壳性状, 转录组

Abstract:

Brown eggs are popular in many countries, and consumers regard eggshell brownness as an important indicator of egg quality. Brown eggshell color is controlled by polygene. However, the responsible genes and detailed molecular mechanisms regulating eggshell brownness have not been defined. In the present study, we applied the RNA-seq technology to analyze the transcriptome data of the shell gland epithelium of hens and investigated the candidate genes associated with eggshell brownness. The results indicated that 8461 genes were expressed in the shell gland epithelium, of which 34 genes were differentially expressed in hens laying dark vs. light brown eggs. Functional analysis revealed that two genes, ovotransferrin (TF) and heat-shock protein 70 (HSP70), as well as the oxidative phosphorylation pathway were involved in the synthesis and transport of protoporphyrin Ⅸ, which might influence the formation of eggshell brownness and result in different shades of brown.

Key words: chicken eggs, eggshell brownness, transcriptome

李光奇, 孙从佼, 吴桂琴, 石凤英, 刘爱巧, 孙皓, 杨宁. 利用转录组测序筛选鸡蛋褐壳性状相关基因[J]. 遗传, 2017, 39(11): 1102-1111.

Li Guangqi, Sun Congjiao, Wu Guiqin, Shi Fengying, Liu Aiqiao, Sun Hao, Yang Ning. Transcriptome sequencing identifies potential regulatory genes involved in chicken eggshell brownness[J]. Hereditas(Beijing), 2017, 39(11): 1102-1111.

参考文献

[1]	Odaba?i AZ, Miles RD, Balaban MO, Portier KM. Changes in brown eggshell color as the hen ages. Poult Sci, 2007, 86(2): 356-363.
[2]	Zhang LC, Ning ZH, Xu GY, Hou ZC, Yang N. Heritabilities and genetic and phenotypic correlations of egg quality traits in brown-egg dwarf layers. Poult Sci, 2005, 84(8): 1209-1213.
[3]	Kennedy GY, Vevers HG. A survey of avian eggshell pigments. Comp Biochem Physiol B, 1976, 55(1): 117-123.
[4]	Lang MR, Wells JW. A review of eggshell pigmentation. World's Poult Sci, 1988, 43(3): 238-246.
[5]	Bhosale SV, Bhosale SV, Shitre GV, Bobe SR, Gupta A. Supramolecular chemistry of protoporphyrin IX and its derivatives. Eur J Org Chem, 2013, 2013(19): 3939-3954.
[6]	Soh T, Koga O. The effects of sex steroid hormones on the pigment accumulation in the shell gland of Japanese quail. Poult Sci, 1994, 73(1): 179-185.
[7]	Wang XT, Deng XM, Zhao CJ, Li JY, Xu GY, Lian LS, Wu CX. Study of the deposition process of eggshell pigments using an improved dissolution method. Poult Sci, 2007, 86(10): 2236-2238.
[8]	Nys Y, Zawadzki J, Gautron J, Mills AD. Whitening of brown-shelled eggs: mineral composition of uterine fluid and rate of protoporphyrin deposition. Poult Sci, 1991, 70(5): 1236-1245.
[9]	Li GQ, Chen SR, Duan ZY, Qu LJ, Xu GY, Yang N. Comparison of protoporphyrin IX content and related gene expression in the tissues of chickens laying brown-shelled eggs. Poult Sci, 2013, 92(12): 3120-3124.
[10]	Punnett RC, Bailey PG. Genetic studies in poultry. J Genet, 1920, 10(4): 277-292.
[11]	Tarazona S, García-Alcalde F, Dopazo J, Ferrer A, Conesa A. Differential expression in RNA-seq: a matter of depth. Genome Res, 2011, 21(12): 2213-2223.
[12]	Chen YS, Xie QM, Qin JP, Zhou QF, Cao YC. Avian important immune factors--chicken β defensin. Chem Life, 2005, 25(6): 501-504.
[12]	陈燕珊, 谢青梅, 覃健萍, 周庆丰, 曹永长. 禽类的重要免疫因子—鸡β防御素. 生命的化学, 2005, 25(6): 501-504.
[13]	Aisen P, Listowsky I. Iron transport and storage proteins. Annu Rev Biochem, 1980, 49: 357-393.
[14]	Williams J. A comparison of glycopeptides from the ovotransferrin and serum transferrin of the hen. Biochem J, 1968, 108(1): 57-67.
[15]	Morimoto RI. Heat shock: the role of transient inducible responses in cell damage, transformation, and differentiation. Cancer Cells, 1991, 3(8): 295-301.
[16]	Green NM. Avidin. Adv Protein Chem, 1975, 29: 85-133.
[17]	Carpenter AR, Becknell MB, Ching CB, Cuaresma EJ, Chen X, Hains DS, McHugh KM Uroplakin 1b is critical in urinary tract development and urothelial differentiation and homeostasis. Kidney Int, 2016, 89(3): 612-624.
[18]	Lee HY, Chung JW, Youn SW, Kim JY, Park KW, Koo BK, Oh BH, Park YB, Chaqour B, Walsh K, Kim HS. Forkhead transcription factor FOXO3a is a negative regulator of angiogenic immediate early gene CYR61, leading to inhibition of vascular smooth muscle cell proliferation and neointimal hyperplasia. Circ Res, 2007, 100(3): 372-380.
[19]	Wang ZP, Qu LJ, Yao JF, Yang XL, Li GQ, Zhang YY, Li JY, Wang XT, Bai JR, Xu GY, Deng XM, Yang N, Wu CX. An EAV-HP insertion in 5′ flanking region of SLCO1B3 causes blue eggshell in the chicken. PLoS Genet, 2013, 9(1): e1003183.
[20]	Wang Z, Gerstein M, Snyder M. RNA-Seq: a revolutionary tool for transcriptomics. Nat Rev Genet, 2009, 10(1): 57-63.
[21]	Qi YX, Liu YB, Rong WH. RNA-Seq and its applications: a new technology for transcriptomics. Hereditas (Beijing), 2011, 33(11): 1191-1202.
[21]	祁云霞, 刘永斌, 荣威恒. 转录组研究新技术: RNA-Seq及其应用. 遗传, 2011, 33(11): 1191-1202.
[22]	Tamura T, Fujii S. Histological observations on the quail oviduct; On the secretions in the mucous epithelium of the uterus. J Fac Fish Anim Husb, 1966, 6: 357-371.
[23]	Zheng CW. Gene expression analysis of brown eggshell pigment formation process and global expression profile microarray analysis[Dissertation]. China Agricultural University, 2013.
[23]	郑传威. 鸡褐壳蛋色素形成过程的基因表达及表达谱芯片分析[Dissertation]. 中国农业大学, 2013.
[24]	Giansanti F, Leboffe L, Pitari G, Ippoliti R, Antonini G. Physiological roles of ovotransferrin. Biochim Biophys Acta, 2012, 1820(3): 218-225.
[25]	Schaeffer E, Lucero MA, Jeltsch JM, Py MC, Levin MJ, Chambon P, Cohen GN, Zakin MM. Complete structure of the human transferrin gene. Comparison with analogous chicken gene and human pseudogene. Gene, 1987, 56(1): 109-116.
[26]	Coll J, Ingram VM. Identification of ovotransferrin as a heme-, colony- and burst-stimulating factor in chick erythroid cell cultures. Exp Cell Res, 1981, 131(1): 173-184.
[27]	Keung WM, Azari P, Phillips JL. Structure and function of ovotransferrin. I. Production of iron-binding fragments from iron-ovotransferrin by the action of immobilized subtilisin. Purification and characterization of the fragments. J Biol Chem, 1982, 257(3): 1177-1183.
[28]	Jordan PM. Highlights in haem biosynthesis. Curr Opin Struct Biol, 1994, 4(6): 902-911.
[29]	Ajioka RS, Phillips JD, Kushner JP. Biosynthesis of heme in mammals. Biochim Biophys Acta, 2006, 1763(7): 723-736.
[30]	Young JC, Agashe VR, Siegers K, Hartl FU. Pathways of chaperone-mediated protein folding in the cytosol. Nat Rev Mol Cell Biol, 2004, 5(10): 781-791.
[31]	Urban-Chmiel R, Puchalski A, Wernicki A, Dec M, Paluch E. Characterization of Hsp70 proteins in bovine leukocytes induced by the temperature 41 degrees C. Pol J Vet Sci, 2009, 12(3): 323-328.
[32]	Semenkov VF, Michalski AI, Sapozhnikov AM. Heating and ultraviolet light activate anti-stress gene functions in humans. Front Genet, 2015, 6: 245.
[33]	Tanaka T, Shibazaki A, Ono R, Kaisho T. HSP70 mediates degradation of the p65 subunit of nuclear factor kappaB to inhibit inflammatory signaling. Sci Signal, 2014, 7(356): ra119.
[34]	Aggarwal A, Ashutosh, Chandra G, Singh AK. Heat shock protein 70, oxidative stress, and antioxidant status in periparturient crossbred cows supplemented with α-tocopherol acetate. Trop Anim Health Prod, 2013, 45(1): 239-245.
[35]	Afonso S, Vanore G, Batlle A. Protoporphyrin IX and oxidative stress. Free Radic Res, 1999, 31(3): 161-170.
[36]	Shan Y, Pepe J, Lu TH, Elbirt KK, Lambrecht RW, Bonkovsky HL. Induction of the heme oxygenase-1 gene by metalloporphyrins. Arch Biochem Biophys, 2000, 380(2): 219-227.
[37]	Moreno J, Osorno JL. Avian egg colour and sexual selection: does eggshell pigmentation reflect female condition and genetic quality?. Ecol Lett, 2003, 6(9): 803-806.
[38]	Martínez-de la Puente J, Merino S, Moreno J, Tomás G, Morales J, Lobato E, García-Fraile S, Martínez J. Are eggshell spottiness and colour indicators of health and condition in blue tits. Cyanistes caeruleus? J Avian Biol, 2007, 38(3): 377-384.
[39]	Brenner DA, Bloomer JR. The enzymatic defect in variegate porphyria. N Engl J Med, 1980, 302(14): 765-769.
[40]	Porra RJ, Falk JE. The enzymic conversion of coproporphyrinogen III into protoporphyrin IX. Biochem J, 1964, 90(1): 69-75.
[41]	Sparks NHC. Eggshell pigments - from formation to deposition. Avian Biol Res, 2011, 4(4): 162-167.
[42]	Calhoun MW, Thomas JW, Gennis RB. The cytochrome oxidase superfamily of redox-driven proton pumps. Trends Biochem Sci, 1994, 19(8): 325-330.
[43]	Michel H, Behr J, Harrenga A, Kannt A. CYTOCHROME C OXIDASE: structure and spectroscopy. Annu Rev Biophys Biomol Struc, 1998, 27: 329-356.
[44]	Hatefi Y. The mitochondrial electron transport and oxidative phosphorylation system. Annu Rev Biochem, 1985, 54: 1015-1069.

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利用转录组测序筛选鸡蛋褐壳性状相关基因

Transcriptome sequencing identifies potential regulatory genes involved in chicken eggshell brownness

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