遗传 ›› 2017, Vol. 39 ›› Issue (11): 1102-1111.doi: 10.16288/j.yczz.17-111

• 研究报告 • 上一篇    下一篇

利用转录组测序筛选鸡蛋褐壳性状相关基因

李光奇1,2(),孙从佼1(),吴桂琴2,石凤英2,刘爱巧2,孙皓2,杨宁1()   

  1. 1. 中国农业大学动物科技学院,北京100193
    2. 北京市华都峪口禽业有限责任公司,北京101206
  • 收稿日期:2017-03-28 修回日期:2017-09-02 出版日期:2017-11-20 发布日期:2017-12-20
  • 作者简介:李光奇,博士,博士后,研究方向：家禽分子遗传育种。E-mail: lgq717535@126.com|孙从佼,博士,讲师,研究方向：家禽分子遗传育种。E-mail: cjsun@cau.edu.cn|杨宁，博士，教授，研究方向：家禽分子遗传育种。E-mail: nyang@cau.edu.cn
  • 基金资助:
    国家自然科学基金项目(31501915);国家蛋鸡产业技术体系项目(2017QC009, 2017DK001);国家蛋鸡产业技术体系项目(CARS-41);长江学者和创新团队发展计划项目(IRT119)

Transcriptome sequencing identifies potential regulatory genes involved in chicken eggshell brownness

Guangqi Li1,2(),Congjiao Sun1(),Guiqin Wu2,Fengying Shi2,Aiqiao Liu2,Hao Sun2,Ning Yang1()   

  1. 1. College of Animal Science and Technology, China Agricultural University, Beijing 100193, China
    2. Beijing Huadu Yukou Poultry Industry Co. Ltd., Beijing 101206, China
  • Received:2017-03-28 Revised:2017-09-02 Online:2017-11-20 Published:2017-12-20
  • Supported by:
    the National Natural Science Foundation of China(31501915);Chinese Universities Scientific Fund(2017QC009, 2017DK001);National Agricultural Technology System (Layer)(CARS-41);Programs for Changjiang Scholars and Innovative Research in University(IRT119)

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摘要：

褐壳鸡蛋在许多国家深受消费者喜爱,消费者通常把蛋壳褐色深浅作为评价鸡蛋品质的重要指标。褐壳鸡蛋蛋壳颜色形成受多基因共同调控,但是具体候选基因及调控机理尚未明确。因此,本研究以纯系褐壳蛋鸡为实验材料,筛选调控褐壳鸡蛋颜色深浅的候选基因。采用转录组测序技术对产深褐壳鸡蛋和浅褐壳鸡蛋的母鸡蛋壳腺组织进行分析,结果显示,共计有8461个基因在蛋壳腺组织表达,其中34个基因在两组之间差异表达。功能分析发现,卵转铁蛋白(ovotransferrin, TF)基因、热休克蛋白70 (heat shock protein, HSP70)基因以及氧化磷酸化通路均与原卟啉Ⅸ合成通路相关,可能影响褐壳鸡蛋蛋壳色素原卟啉Ⅸ的合成和积累。

关键词: 鸡蛋, 褐壳性状, 转录组

Abstract:

Brown eggs are popular in many countries, and consumers regard eggshell brownness as an important indicator of egg quality. Brown eggshell color is controlled by polygene. However, the responsible genes and detailed molecular mechanisms regulating eggshell brownness have not been defined. In the present study, we applied the RNA-seq technology to analyze the transcriptome data of the shell gland epithelium of hens and investigated the candidate genes associated with eggshell brownness. The results indicated that 8461 genes were expressed in the shell gland epithelium, of which 34 genes were differentially expressed in hens laying dark vs. light brown eggs. Functional analysis revealed that two genes, ovotransferrin (TF) and heat-shock protein 70 (HSP70), as well as the oxidative phosphorylation pathway were involved in the synthesis and transport of protoporphyrin Ⅸ, which might influence the formation of eggshell brownness and result in different shades of brown.

Key words: chicken eggs, eggshell brownness, transcriptome