鸡3种组织中热应激相关基因的表达谱芯片分析

doi:10.3724/SP.J.1005.2014.0800

遗传 ›› 2014, Vol. 36 ›› Issue (8): 800-808.doi: 10.3724/SP.J.1005.2014.0800

鸡3种组织中热应激相关基因的表达谱芯片分析

宋晓燕^{1, 3}, 张德祥^{1, 2}, 张文武^{1, 3}, 季从亮², 张细权^{1, 3}, 罗庆斌^{1, 3}

1. 华南农业大学,动物科学学院,广州 510642;
2. 广东温氏食品集团股份有限公司,新兴 527439;
3. 华南农业大学,农业部鸡遗传育种与繁殖重点实验室,广州 510642

收稿日期:2013-11-18 出版日期:2014-08-20 发布日期:2013-07-19
通讯作者: 罗庆斌,副教授,研究方向：分子遗传与动物育种。E-mail:qbluo@scau.edu.cn
作者简介:宋晓燕,硕士,专业方向：分子遗传与动物育种。E-mail: 85468640@qq.com
基金资助:
国家自然科学基金项目(编号：30972093)和国家科技支撑计划项目(编号：2014BAD08B08)资助

Gene expression profiling of three tissues of chicken after heat stress treatment by microarray technique

Xiaoyan Song^{1, 3}, Dexiang Zhang^{1, 2}, Wenwu Zhang^{1, 3}, Congliang Ji², Xiquan Zhang^{1, 3}, Qingbin Luo^{1, 3}

1. College of Animal Science, South China Agricultural University, Guangzhou 510642, China;
2. Guangdong Wens Food Group Limited Company, Xinxing 527439, China;
3. Key Lab of Chicken Genetics, Breeding and Reproduction, Ministry of Agriculture, South China Agricultural University,Guangzhou 510642, China

Received:2013-11-18 Online:2014-08-20 Published:2013-07-19

摘要/Abstract

摘要： 文章以8日龄矮小型黄羽肉雏鸡为材料,以28℃±1℃处理3 h为对照组,40℃±1℃热应激3 h为实验组,采集大脑、肝脏、腿肌3种组织,利用全基因组表达谱芯片检测实验组和对照组各组织的差异表达基因,并结合生物信息学方法分析其功能。研究结果表明：在热应激处理组与对照组中,大脑组织中共有183个显著变化的差异表达探针(变化倍数≥2或≤0.5,q≤0.05),129个有基因注释,39个生物学过程发生了改变,18个生物学通路发生显著改变;肝脏组织中有558个显著改变的探针(变化倍数≥2或≤0.5,q≤0.05),包含339个基因,涉及37个生物学过程,58个生物学通路发生改变;腿肌组织中显著改变的探针有301个(变化倍数≥2或≤0.5,q≤0.05),209个有基因注释,参与了31个生物学过程,51个生物学通路发生改变。3种组织中,获得差异表达探针935个,不同组织具有特异性的表达模式;多种热休克蛋白家族基因被诱导上调表达,抗凋亡过程、钙离子信号通路、MAPK信号通路和多种代谢途径发生改变。文章对鸡的热应激分子机制进行了初步探索,为筛选耐热性相关基因提供了理论依据。

关键词: 鸡, 热应激, 表达谱, 基因芯片

Abstract: In the present study, tissue samples were collected from the cerebrum, liver, and leg muscle of 8-day-old dwarf chicks that were exposed to a 3 h treatment of 28℃± 1℃ (control group) or 40℃± 1℃ (treatment group). Differentially expressed (DE) genes in these samples were detected using whole-genome microarray chips, and their functions were

Key words: chicken, heat stress, gene expression profiling, gene chip

宋晓燕, 张德祥, 张文武, 季从亮, 张细权, 罗庆斌. 鸡3种组织中热应激相关基因的表达谱芯片分析[J]. 遗传, 2014, 36(8): 800-808.

Xiaoyan Song, Dexiang Zhang, Wenwu Zhang, Congliang Ji, Xiquan Zhang, Qingbin Luo. Gene expression profiling of three tissues of chicken after heat stress treatment by microarray technique[J]. HEREDITAS(Beijing), 2014, 36(8): 800-808.

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鸡3种组织中热应激相关基因的表达谱芯片分析

Gene expression profiling of three tissues of chicken after heat stress treatment by microarray technique

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