遗传 ›› 2016, Vol. 38 ›› Issue (5): 402-410.doi: 10.16288/j.yczz.15-466
敖政, 刘德武, 蔡更元, 吴珍芳, 李紫聪
收稿日期:
2015-11-12
修回日期:
2015-12-29
出版日期:
2016-05-20
发布日期:
2016-02-23
通讯作者:
李紫聪,博士,副教授,研究方向:动物遗传育种与繁殖。E-mail: lizicongcong@163.com
E-mail:zheng780911@163.com
作者简介:
敖政,硕士研究生,研究方向:分子遗传育种。E-mail: zheng780911@163.com
Zheng Ao, Dewu Liu, Gengyuan Cai, Zhenfang Wu, Zicong Li
Received:
2015-11-12
Revised:
2015-12-29
Online:
2016-05-20
Published:
2016-02-23
摘要: 克隆又称体细胞核移植(Somatic cell nuclear transfer, SCNT),该技术已在多种哺乳动物中成功建立并被逐渐应用。然而,利用该技术获得的哺乳动物克隆胚胎的发育效率非常低(一般只有1%~5%),这严重限制了克隆技术的应用。胎盘发育缺陷被认为是抑制克隆胚胎发育的一个主要原因。几乎所有的SCNT来源胎盘都会出现不同的发育缺陷,如胎盘增生、胎盘血管缺陷、脐带畸形等。胎盘异常的根本原因是滋养层细胞基因组在发育过程中未能建立正确的表观遗传修饰,导致胎盘发育调控相关的重要基因,特别是印迹基因的表达出现异常。印迹基因表达异常导致胎盘的形态异常和功能缺陷,进而影响克隆胚胎的发育能力。目前,虽然有许多提高克隆胚胎发育能力的研究报道,然而在大多数研究中克隆效率并没有得到大幅度的提高,主要原因之一是克隆胚胎的胎盘发育仍然存在诸多缺陷。本文综述了克隆哺乳动物的胎盘异常及其印迹基因表达,并对未来提高克隆效率的研究提出展望。
敖政, 刘德武, 蔡更元, 吴珍芳, 李紫聪. 克隆哺乳动物的胎盘发育缺陷[J]. 遗传, 2016, 38(5): 402-410.
Zheng Ao, Dewu Liu, Gengyuan Cai, Zhenfang Wu, Zicong Li. Placental developmental defects in cloned mammalian animals[J]. HEREDITAS(Beijing), 2016, 38(5): 402-410.
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