CRISPR/Cas9系统在培育抗病毒植物新种质中的应用

doi:10.16288/j.yczz.16-134

遗传 ›› 2016, Vol. 38 ›› Issue (9): 811-820.doi: 10.16288/j.yczz.16-134

CRISPR/Cas9系统在培育抗病毒植物新种质中的应用

张道微¹, 张超凡^{1, 2}, 董芳², 黄艳岚¹, 张亚¹, 周虹¹

1. 湖南省作物研究所,长沙 410125;
2. 中南大学研究生院隆平分院,长沙 410125

收稿日期:2016-04-18 出版日期:2016-09-20 发布日期:2016-09-20
通讯作者: 张超凡,博士研究生,研究员,研究方向：甘薯育种与栽培。E-mail:cfzhang2004@sina.com
作者简介:张道微,硕士研究生,助理研究员,研究方向：甘薯育种与甘薯病毒病防治。E-mail:zhang000048@126.com
基金资助:
国家甘薯产业技术体系项目(编号：CARS-11-C-16) 和湖南省旱粮产业技术体系（病虫害防控岗位）项目资助

Application of CRISPR/Cas9 system in breeding of new antiviral plant germplasm

Daowei Zhang¹, Chaofan Zhang^{1, 2}, Fang Dong², Yanlan Huang¹, Ya Zhang¹, Hong Zhou¹

1. Hunan Crop Research Institute, Changsha 410125, China;
2. Longping Branch of Graduate School of Central South University, Changsha 410125, China

Received:2016-04-18 Online:2016-09-20 Published:2016-09-20
Supported by:
[Supported by China Agriculture Research System of Sweet Potato (No; CARS-11-C-16) and Agriculture Research System of Upland Crops in Hunan Province (the Post of Prevention and Control of Pest and Disease)]

摘要/Abstract

摘要： 随着CRISPR/Cas9系统在基因组编辑技术上的开发和完善,CRISPR/Cas9系统在应用于动物病毒感染性疾病防治并取得相当成效的同时,也逐步被应用到对植物病毒基因组进行高效靶向修饰的研究中。CRISPR/Cas9系统对基因组靶向修饰作用不仅实现了对植物DNA病毒基因组序列的编辑,还展示了其有效作用于植物RNA病毒基因组的潜力,同时CRISPR/Cas9系统还能在基因转录和转录后调控水平发挥作用,说明该系统具有通过多种途径调控植物病毒复制的潜能。相对其他植物病毒病防治策略,该系统对病毒基因组的编辑更精准、对基因表达的调控更稳定,对病毒病的抗性也更为广谱。本文将CRISPR/Cas9系统与其他植物病毒病防治策略进行了比较,概述了该系统在培育植物抗病毒病新种质中的优势,分析了其具体应用在该领域中面临的主要问题,讨论了该系统在培育抗病毒植物新种质应用中的发展趋势。

关键词: CRISPR/Cas9系统, 病毒病防治, 基因组靶向修饰

Abstract: With the development and improvement of CRISPR/Cas9 system in genomic editing technology, the system has been applied to the prevention and control of animal viral infectious diseases, which has made considerable achievements. It has also been applied to the study of highly efficient gene targeting editing in plant virus genomes. The CRISPR/Cas9-mediated targeted gene modification has not only achieved the genome editing of plant DNA virus, but also showed the genome editing potential of plant RNA virus. In addition, the CRISPR/Cas9 system functions at the gene transcriptional and post-transcriptional level, indicating that the system could regulate the replication of plant viruses through different ways. Compared with other plant viral disease control strategies, this system is more accurate in genome editing, more stable in gene expression regulation, and has broader spectrum of resistance to virus disease. In this review, we summarized the advantages, main problems and development tendency of CRISPR/cas9 system in breeding of new antiviral plant germplasms.

Key words: CRISPR/Cas9 System, virus disease prevention and control, genome targeting modification

张道微, 张超凡, 董芳, 黄艳岚, 张亚, 周虹. CRISPR/Cas9系统在培育抗病毒植物新种质中的应用[J]. 遗传, 2016, 38(9): 811-820.

Daowei Zhang, Chaofan Zhang, Fang Dong, Yanlan Huang, Ya Zhang, Hong Zhou. Application of CRISPR/Cas9 system in breeding of new antiviral plant germplasm[J]. Hereditas(Beijing), 2016, 38(9): 811-820.

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CRISPR/Cas9系统在培育抗病毒植物新种质中的应用

Application of CRISPR/Cas9 system in breeding of new antiviral plant germplasm

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