遗传 ›› 2008, Vol. 30 ›› Issue (4): 491-494.doi: 10.3724/SP.J.1005.2008.00491

• 研究报告 • 上一篇    下一篇

来自粗山羊草抗条锈病基因的SSR标记

张海泉1, 贾继增2, 杨虹1, 张宝石3   

  1. 1. 河北经贸大学生物科学与工程学院, 石家庄 050061;
    2. 中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程, 北京 100081;
    3. 沈阳农业大学农学院, 沈阳 110161

  • 收稿日期:2007-09-25 修回日期:2008-01-14 出版日期:2008-04-10 发布日期:2008-04-10
  • 通讯作者: 张海泉

SSR mapping of stripe rust resistance gene from Ae. tauschii

ZHANG Hai-Quan1, JIA Ji-Zeng2, YANG Hong1, ZHANG Bao-Shi3

  

  1. 1. College of Biology Engineering, Hebei University of Economics and Business, Shijiazhuang 050021, China;
    2. Institute of Crop Sciences/The National Key Facilities for Crop Genetic Resources and Improvement, CAAS, Beijing 100081, China;
    3. College of Shenyang Agricultural University, Shenyang 110161, China
  • Received:2007-09-25 Revised:2008-01-14 Online:2008-04-10 Published:2008-04-10
  • Contact: ZHANG Hai-Quan

摘要:

从粗山羊草[Aegilops tauschii (Coss.) Schmal] Y201中鉴定出1个显性抗小麦条锈病基因, 暂定名为YrY201。应用分离群体分组法(BSA) 筛选到Xgwm273b、Xgwm37和wmc14标记, 与该基因之间的遗传距离分别为11.9、5.8和10.9 cM。根据连锁标记所在小麦微卫星图谱的位置, YrY201被定位在7DL染色体上。分析基因所在染色体的位置及抗病性特征, 认为YrY201是一个新的抗小麦条锈病基因,并可用于分子标记辅助选择。

关键词: 粗山羊草, 小麦条锈病, 基因定位

Abstract:

A dominant wheat stripe rust resistance gene, temporarily designated as YrY201, was identified in an accession Y201 of Aegilops tauschii. By bulk segregation analysis, three microsatellite markers Xgwm273b, Xgwm37 and Wmc14 were found to be linked to YrY201 with genetic distance of 11.5, 5.8 and 10.9 cM , respectively. According to the locations of the linked markers, the resistance gene was located on chromosome 7DL. Based on the chromosomal location and the resistance pattern of the gene, we proposed that YrY201 was a novel stripe rust resistance gene, and cuold be selected by marker-assisted selection.