水稻“光身”突变体glr3的遗传分析及基因定位

doi:10.16288/j.yczz.16-114

遗传 ›› 2016, Vol. 38 ›› Issue (11): 1012-1019.doi: 10.16288/j.yczz.16-114

水稻“光身”突变体glr3的遗传分析及基因定位

宋海冰^{1, 2}, 汪斌^{1, 3}, 陈壬杰^{1, 2}, 郑小雅^{1, 2}, 于世波^{1, 2}, 兰涛^{1, 2}

1. 福建农林大学,作物遗传育种与综合利用省部共建教育部重点实验室,福州 350002;
2. 福建农林大学,福建省作物设计育种重点实验室,福州 350002;
3. 福建农林大学生命科学学院,福州 350002

收稿日期:2016-04-05 出版日期:2016-11-20 发布日期:2016-08-23
通讯作者: 兰涛,博士,副教授,研究方向：植物抗逆遗传。E-mail: tlan@fafu.edu.cn
作者简介:宋海冰,硕士研究生,专业方向：作物遗传育种。E-mail: 359867517@qq.com;汪斌,博士,副教授,研究方向：遗传学。E-mail: wangbin_doc@163.com;宋海冰和汪斌为并列第一作者。
基金资助:
国家自然科学基金项目(编号：31671688),福建省自然科学基金项目(编号：2015J01094)和福建省教育厅科技计划项目(编号：JK2012014)资助

Genetic analysis and gene mapping of the glabrous leaf and hull mutant glr3 in rice (Oryza sativa L.)

Haibing Song^{1, 2}, Bin Wang^{1, 3}, Renjie Chen^{1, 2}, Xiaoya Zheng^{1, 2}, Shibo Yu^{1, 2}, Tao Lan^{1, 2}

1. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. Fujian Provincial Key Laboratory of Crop Breeding by Design, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
3. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Received:2016-04-05 Online:2016-11-20 Published:2016-08-23
Supported by:
[Supported by the National Natural Science Foundation of China(No. 31671688), Natural Science Foundation of Fujian Province(No. 2015J01094) and Science and Technology Project of the Education Department of Fujian Province(No. JK2012014)]

摘要/Abstract

摘要： 在籼稻品种R401辐射诱变的M₂群体中筛选到一个叶片表皮光滑突变体,在正常条件下,突变体叶片和颖壳光滑无毛。以毛叶粳稻品种Nipponbare和“光身”突变体作为亲本,构建了一个F₂群体,通过调查该群体在正常种植条件下的表现,发现Nipponbare和“光身”突变体控制表皮毛性状的差异受单个主基因控制且“光身”为隐性,将该基因暂时命名为GLR3。利用该F₂群体,采用BSA法将GLR3定位在第6染色体上,进一步对F₂群体中417个典型的叶片光滑单株进行分子标记分析,将该基因定位在InDel标记ID27101和ID27199之间,与两标记相距皆为0.1 cM,两标记的物理位置相距98 kb。

关键词: 水稻, 光身, 遗传分析, 基因定位

Abstract: We obtained a glabrous leaf and hull mutant from a population of radiation mutagenesis of an indica rice cultivar R401. The mutant produced smooth leaves and hairless glumes under normal growth conditions. An F₂ population was developed from a cross between a japonica cultivar Nipponbare and the glabrous leaf and hull mutant. By investigating the performance of the F₂ population, we found that the mutant phenotype was controlled by a single recessive gene, temporarily designated GLR3. Bulked segregant analysis (BSA) based on the F₂ mapping population revealed that GLR3 is located on chromosome 6. By analyzing 417 typical glabrous leaf F₂ plants using molecular markers, GLR3 was mapped to a 0.2 cM interval between InDel markers ID27101 and ID27199, and the physical distance between the two markers is 98 kb. Thus we have mapped the gene GLR3, and our work will provide basis for future mechanistic analysis of GLR3 function.

Key words: rice, glabrous leaf and hull, genetic analysis, gene mapping

宋海冰, 汪斌, 陈壬杰, 郑小雅, 于世波, 兰涛. 水稻“光身”突变体glr3的遗传分析及基因定位[J]. 遗传, 2016, 38(11): 1012-1019.

Haibing Song, Bin Wang, Renjie Chen, Xiaoya Zheng, Shibo Yu, Tao Lan. Genetic analysis and gene mapping of the glabrous leaf and hull mutant glr3 in rice (Oryza sativa L.)[J]. Hereditas(Beijing), 2016, 38(11): 1012-1019.

参考文献

[1] Zhao CZ, Yang CD, Wu LB, Qi XF, Huang FS, Hu PS, Luo J. Studies of culture character in glabrous rice ( Oryza sativa L.). Acta Agron Sin , 1999, 25(1): 82-85. 赵成章, 杨长登, 吴连斌, 戚秀芳, 黄发松, 胡培松, 罗矩. 光身稻的培养特性研究. 作物学报, 1999, 25(1): 82-85.
[2] Johnson HB. Plant pubescence: An ecological perspective. Bot Rev , 1975, 41(3): 233-258.
[3] Szymanski DB, Lloyd AM, Marks MD. Progress in the molecular genetic analysis of trichome initiation and morphogenesis in Arabidopsis . Trends Plant Sci , 2000, 5(5): 214-219.
[4] Pu L, Suo JF, Xue YB. Molecular control of plant trichome development. Acta Genet Sin , 2003, 30(11): 1078-1084. 普莉, 索金凤, 薛勇彪. 植物表皮毛发育的分子遗传控制. 遗传学报, 2003, 30(11): 1078-1084.
[5] Serna L, Martin C. Trichomes: different regulatory networks lead to convergent structures. Trends Plant Sci , 2006, 11(6): 274-280.
[6] Ishida T, Kurata T, Okada K, Wada T. A genetic regulatory network in the development of trichomes and root hairs. Annu Rev Plant Biol , 2008, 59: 365-386.
[7] Yan A, Pan JB, An LZ, Gan YB, Feng HY. The responses of trichome mutants to enhanced ultraviolet-B radiation in Arabidopsis thaliana . J Photochem Photobiol B , 2012, 113: 29-35.
[8] Herman PL, Marks MD. Trichome development in Arabidopsis thaliana . II. Isolation and complementation of the GLABROUS1 gene. Plant Cell , 1989, 1(11): 1051-1055.
[9] Rerie WG, Feldmann KA, Marks MD. The GLABRA2 gene encodes a homeo domain protein required for normal trichome development in Arabidopsis . Genes Dev , 1994, 8(12): 1388-1399.
[10] Zhao HT, Wang XX, Zhu DD, Cui SJ, Li X, Cao Y, Ma LG. A single amino acid substitution in IIIf subfamily of basic Helix-Loop-Helix transcription factor AtMYC1 leads to trichome and root hair patterning defects by abolishing its interaction with partner proteins in Arabidopsis . J Biol Chem , 2012, 287(17): 14109-14121.
[11] Walker AR, Davison PA, Bolognesi-Winfield AC, James CM, Srinivasan N, Blundell TL, Esch JJ, Marks MD, Gray JC. The TRANSPARENT TESTA GLABRA1 locus, which regulates trichome differentiation and anthocyanin biosynthesis in Arabidopsis , encodes a WD40 repeat protein. Plant Cell , 1999, 11(7): 1337-1350.
[12] Di Cristina M, Sessa G, Dolan L, Linstead P, Baima S, Ruberti I, Morelli G. The Arabidopsis Athb-10 (GLABRA2) is an HD-Zip protein required for regulation of root hair development. Plant J , 2002, 10(3): 393-402.
[13] Johnson CS, Kolevski B, Smyth DR. TRANSPARENT TESTA GLABRA2 , a trichome and seed coat development gene of Arabidopsis, encodes a WRKY transcription factor. Plant Cell , 2002, 14(6): 1359-1375.
[14] Hülskamp M, Miséra S, Jürgens G. Genetic dissection of trichome cell development in Arabidopsis . Cell , 1994, 76(3): 555-566.
[15] Yu N, Cai WJ, Wang SC, Shan CM, Wang LJ, Chen XY. Temporal control of trichome distribution by microRNA156-targeted SPL genes in Arabidopsis thaliana . Plant Cell , 2010, 22(7): 2322-2335.
[16] Gan LJ, Xia K, Chen JG, Wang SC. Functional characterization of TRICHOMELESS2, a new single-repeat R3 MYB transcription factor in the regulation of trichome patterning in Arabidopsis . BMC Plant Biol , 2011, 11: 176.
[17] Matías-Hernández L, Aguilar-Jaramillo AE, Osnato M, Weinstain R, Shani E, Suárez-López P, Pelaz S. TEMPRANILLO reveals the mesophyll as crucial for epidermal trichome formation. Plant Physiol , 2016, 170(3): 1624-1639.
[18] Gao Y, Gong XM, Cao WH, Zhao JF, Fu LQ, Wang XC, Schumaker KS, Guo Y. SAD2 in Arabidopsis functions in trichome initiation through mediating GL3 function and regulating GL1 , TTG1 and GL2 expression. J Integr Plant Biol , 2008, 50(7): 906-917.
[19] Reiner T, Hoefle C, Huesmann C, Ménesi D, Fehér A, Hückelhoven R. The Arabidopsis ROP-activated receptor-like cytoplasmic kinase RLCK VI_A3 is involved in control of basal resistance to powdery mildew and trichome branching. Plant Cell Rep , 2015, 34(3): 457-468.
[20] Yu DS, Yu F, Du CQ, Li XS, Zhao XY, Liu XM. RPN1a, a subunit of the 26S proteasome, controls trichome development in Arabidopsis . Plant Physiol Biochem , 2015, 88: 82-88.
[21] Li JJ, Lin ZK, Chen HZ, Xu ML. The inheritance of glabrous-leaf character in rice variety Rico No.1. Acta Agric Zhejiangensis , 1993, 5(4): 233-234. 李金军, 林正魁, 陈鸿藻, 徐美玲. 水稻品种Rico No.1光叶特性的遗传. 浙江农业学报, 1993, 5(4): 233-234.
[22] Qian Q, He P, Zheng XW, Chen Y, Zhu LH. Genetic analysis of morphological index and its related taxonomic traits for classification of indica/japonica rice. Sci China Ser C , 2000, 43(2): 113-119.
[23] Li WQ, Wu JG, Weng SL, Zhang DP, Zhang YJ, Shi CH. Characterization and fine mapping of the glabrous leaf and hull mutants ( gl1 ) in rice ( Oryza sativa L.). Plant Cell Rep , 2010, 29(6): 617-627.
[24] Hong J, Wang QZ, Fu HW, Wu DX, Shu QY. Fine mapping and candidate gene analysis of glabrous leaf and hull gene ( gl 1) in rice ( Oryza sativa L.). J Nucl Agric Sci , 2011, 25(6): 1088-1093. 洪隽, 王启钊, 富昊伟, 吴殿星, 舒庆尧. 水稻光叶性状基因 gl 1的精细定位与候选基因分析. 核农学报, 2011, 25(6): 1088-1093.
[25] Li JJ, Yuan YD, Lu ZF, Yang LS, Gao RC, Lu JG, Li JY, Xiong GS. Glabrous Rice 1 , encoding a homeodomain protein, regulates trichome development in rice. Rice , 2012, 5: 32.
[26] Zhang HL, Wu K, Wang YF, Peng Y, Hu FY, Wen L, Han B, Qian Q, Teng S. A WUSCHEL -like homeobox gene, OsWOX3B responses to NUDA/GL-1 locus in rice. Rice , 2012, 5: 30.
[27] Angeles-Shim RB, Asano K, Takashi T, Shim J, Kuroha T, Ayano M, Ashikari M. A WUSCHEL-related homeobox 3B gene, depilous ( dep ), confers glabrousness of rice leaves and glumes. Rice , 2012, 5: 28.
[28] Wang YP, Chen WL, Qin P, Huang YY, Ma BT, Ouyang XH, Chen XW, Li SG. Characterization and fine mapping of GLABROUS RICE 2 in rice. J Genet Genomics , 2013, 40(11): 579-582.
[29] Zeng YH, Zhu YS, Lian L, Xie HG, Zhang JF, Xie HA. Genetic analysis and fine mapping of the pubescence gene GL6 in rice ( Oryza sativa L.). Chin Sci Bull , 2013, 58(24): 2992-2999.
[30] Dongchen WH, Zhang XL, Zhu Q, Xiong HB, Wei ZF, Lv YG, Zhang LD, Wu TF, Li W, Wu C, Chen LJ, Lee D. Cloning and subcellular localization of a new glabrous-leaf mutant gene GLL in rice ( Oryzα sativα L.). Mol Plant Breed , 2015, 13(4): 716-726. 董陈文华, 张小玲, 朱骞, 熊海波, 魏振飞, 吕永刚, 张利东, 伍腾飞, 李伟, 吴超, 陈丽娟, 李东宣. 水稻光叶突变新基因的克隆和亚细胞定位. 分子植物育种, 2015, 13(4): 716-726.
[31] Zhu XB, Sun DY, Cheng BS, Hong DL. Distribution characterization of leaf and hull pubescences and genetic analysis of their numbers in japonica rice ( Oryza sativa ). Rice Sci , 2008, 15(4): 267-275.
[32] Lan T, Liang KJ, Chen ZW, Duan YL, Wang JL, Ye N, Wu WR. Genetic analysis and gene mapping of cold-induced seedling chlorosis in rice. Hereditas ( Beijing ), 2007, 29(9): 1121-1125. 兰涛, 梁康迳, 陈志伟, 段远霖, 王俊兰, 叶宁, 吴为人. 水稻苗期低温失绿的遗传分析及基因定位. 遗传, 2007, 29(9): 1121-1125.
[33] Ströher E, Grassl J, Carrie C, Fenske R, Whelan J, Millar AH. Glutaredoxin S15 is involved in Fe-S cluster transfer in mitochondria influencing lipoic acid-dependent enzymes, plant growth, and arsenic tolerance in Arabidopsis . Plant Physiol , 2016, 170(3): 1284-1299.
[34] Walters LA, Escobar MA. The AtGRXS3/4/5/7/8 glutaredoxin gene cluster on Arabidopsis thaliana chromosome 4 is coordinately regulated by nitrate and appears to control primary root growth. Plant Signal Behav , 2016, 11(4): e1171450.
[35] Yokota E, Vidali L, Tominaga M, Tahara H, Orii H, Morizane Y, Hepler PK, Shimmen T. Plant 115-kDa actin-filament bundling protein, P-115-ABP, is a homologue of plant villin and is widely distributed in cells. Plant Cell Physiol , 2003, 44(10): 1088-1099.
[36] Yokota E, Tominaga M, Mabuchi I, Tsuji Y, Staiger CJ, Oiwa K, Shimmen T. Plant villin, lily P-135-ABP, possesses G-actin binding activity and accelerates the polymerization and depolymerization of actin in a Ca 2+ -sensitive manner. Plant Cell Physiol , 2005, 46(10): 1690-1703.
[37] Sun PL, Li S, Lu DH, Williams JS, Kao TH. Pollen S-locus F-box proteins of Petunia involved in S-RNase-based self-incompatibility are themselves subject to ubiquitin-mediated degradation. Plant J , 2015, 83(2): 213-223.
[38] Noir S, Marrocco K, Masoud K, Thomann A, Gusti A, Bitrian M, Schnittger A, Genschik P. The control of Arabidopsis thaliana growth by cell proliferation and endoreplication requires the F-Box protein FBL17. Plant Cell , 2015, 27(5): 1461-1476.
[39] Cardon GH, Höhmann S, Nettesheim K, Saedler H, Huijser P. Functional analysis of the Arabidopsis thaliana SBP-box gene SPL3 : a novel gene involved in the floral transition. Plant J , 1997, 12(2): 367-377.
[40] Cardon G, Höhmann S, Klein J, Nettesheim K, Saedler H, Huijser P. Molecular characterisation of the Arabidopsis SBP-box genes. Gene , 1999, 237(1): 91-104.
[41] Wu G, Park MY, Conway SR, Wang JW, Weigel D, Poethig RS. The sequential action of miR156 and miR172 regulates developmental timing in Arabidopsis . Cell , 2009, 138(4): 750-759
[42] Guo AY, Zhu QH, Gu XC, Ge S, Yang J, Luo JC. Genome-wide identifiﬁcation and evolutionary analysis of the plant speciﬁc SBP-box transcription factor family. Gene , 2008, 418(1-2): 1-8.
[43] Unte US, Sorensen AM, Pesaresi P, Gandikota M, Leister D, Saedler H, Huijser P. SPL8 , an SBP-Box gene that affects pollen sac development in Arabidopsis . Plant Cell , 2003, 15(4): 1009-1019.
[44] Zhang Y, Schwarz S, Saedler H, Huijser P. SPL8, a local regulator in a subset of gibberellin-mediated developmental processes in Arabidopsis . Plant Mol Biol , 2007, 63(3): 429-439.

编辑推荐

Metrics

www.chinagene.cn
备案号：京ICP备09063187号-4
总访问:,今日访问:,当前在线:

水稻“光身”突变体glr3的遗传分析及基因定位

Genetic analysis and gene mapping of the glabrous leaf and hull mutant glr3 in rice (Oryza sativa L.)

PDF (PC)

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

[1]	张桂权. 基于SSSL文库的水稻设计育种平台[J]. 遗传, 2019, 41(8): 754-760.
[2]	王春明, 孙英莉, 林昌俊, 王铭裕, 牛月, 李晓峰, 冯虎元. 粗糙脉孢菌7种子囊型归类教学探究[J]. 遗传, 2019, 41(11): 1067-1072.
[3]	刘次桃, 王威, 毛毕刚, 储成才. 水稻耐低温逆境研究：分子生理机制及育种展望[J]. 遗传, 2018, 40(3): 171-185.
[4]	杨德卫, 郑向华, 程朝平, 叶宁, 黄凤凰, 叶新福. 基于CSSLs群体定位和图位克隆水稻长芒基因GAD1-2[J]. 遗传, 2018, 40(12): 1101-1111.
[5]	辛高伟, 胡熙璕, 王克剑, 王兴春. Cas9蛋白变体VQR高效识别水稻NGAC前间区序列邻近基序[J]. 遗传, 2018, 40(12): 1112-1119.
[6]	吴比, 胡伟, 邢永忠. 中国水稻遗传育种历程与展望[J]. 遗传, 2018, 40(10): 841-857.
[7]	李佳,刘运华,张余,陈晨,余霞,余舜武. 干旱对水稻生物钟基因和干旱胁迫响应基因每日节律性变化的影响[J]. 遗传, 2017, 39(9): 837-846.
[8]	韩晓斌, 徐冉, 段朋根, 于海跃, 罗越华, 李云海. 水稻斑点叶突变体spl101和spl102的筛选及候选基因鉴定[J]. 遗传, 2017, 39(4): 346-353.
[9]	唐丽, 李曜魁, 张丹, 毛毕刚, 吕启明, 胡远艺, 韶也, 彭彦, 赵炳然, 夏石头. 基于基因组编辑技术的水稻靶向突变特征及遗传分析[J]. 遗传, 2016, 38(8): 746-755.
[10]	武迪, 黄林周, 高谨, 王永红. 植物重力反应的分子调控机制[J]. 遗传, 2016, 38(7): 589-602.
[11]	孔德艳, 陈守俊, 周立国, 高欢, 罗利军, 刘灶长. 水稻开花光周期调控相关基因研究进展[J]. 遗传, 2016, 38(6): 532-542.
[12]	李莉云,史佳楠,杨烁,孙财强,刘国振. 基于转录特征的水稻WRKY转录因子功能注释[J]. 遗传, 2016, 38(2): 126-136.
[13]	张红宇, 崔晓云, 侯飞雪, 王一伊, 吴挺开, 刘禹彤, 杨定乾, 张洪凯, 傅瑶, 张向阳, 李文丽, 吴先军. 水稻基因组加倍对籽粒大小调控基因表达的影响[J]. 遗传, 2016, 38(12): 1102-1111.
[14]	胡运高, 郭连安, 杨国涛, 钦鹏, 范存留, 彭友林, 严维, 何航, 李仕贵. 直立密穗基因DEP2-1388的遗传分析及在杂交稻中的育种利用[J]. 遗传, 2016, 38(1): 72-81.
[15]	储黄伟, 牛付安, 程灿, 周继华, 王新其, 罗小金, 袁勤, 曹黎明. 杂交粳稻花优14及其亲本孕穗期剑叶的基因表达谱芯片分析[J]. 遗传, 2015, 37(9): 932-938.