水稻“光身”突变体glr3的遗传分析及基因定位

doi:10.16288/j.yczz.16-114

遗传 ›› 2016, Vol. 38 ›› Issue (11): 1012-1019.doi: 10.16288/j.yczz.16-114

水稻“光身”突变体glr3的遗传分析及基因定位

宋海冰^{1, 2}, 汪斌^{1, 3}, 陈壬杰^{1, 2}, 郑小雅^{1, 2}, 于世波^{1, 2}, 兰涛^{1, 2}

1. 福建农林大学,作物遗传育种与综合利用省部共建教育部重点实验室,福州 350002;
2. 福建农林大学,福建省作物设计育种重点实验室,福州 350002;
3. 福建农林大学生命科学学院,福州 350002

收稿日期:2016-04-05 出版日期:2016-11-20 发布日期:2016-08-23
通讯作者: 兰涛,博士,副教授,研究方向：植物抗逆遗传。E-mail: tlan@fafu.edu.cn
作者简介:宋海冰,硕士研究生,专业方向：作物遗传育种。E-mail: 359867517@qq.com;汪斌,博士,副教授,研究方向：遗传学。E-mail: wangbin_doc@163.com;宋海冰和汪斌为并列第一作者。
基金资助:
国家自然科学基金项目(编号：31671688),福建省自然科学基金项目(编号：2015J01094)和福建省教育厅科技计划项目(编号：JK2012014)资助

Genetic analysis and gene mapping of the glabrous leaf and hull mutant glr3 in rice (Oryza sativa L.)

Haibing Song^{1, 2}, Bin Wang^{1, 3}, Renjie Chen^{1, 2}, Xiaoya Zheng^{1, 2}, Shibo Yu^{1, 2}, Tao Lan^{1, 2}

1. Key Laboratory of Ministry of Education for Genetics, Breeding and Multiple Utilization of Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
2. Fujian Provincial Key Laboratory of Crop Breeding by Design, Fujian Agriculture and Forestry University, Fuzhou 350002, China;
3. College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Received:2016-04-05 Online:2016-11-20 Published:2016-08-23
Supported by:
[Supported by the National Natural Science Foundation of China(No. 31671688), Natural Science Foundation of Fujian Province(No. 2015J01094) and Science and Technology Project of the Education Department of Fujian Province(No. JK2012014)]

摘要/Abstract

摘要： 在籼稻品种R401辐射诱变的M₂群体中筛选到一个叶片表皮光滑突变体,在正常条件下,突变体叶片和颖壳光滑无毛。以毛叶粳稻品种Nipponbare和“光身”突变体作为亲本,构建了一个F₂群体,通过调查该群体在正常种植条件下的表现,发现Nipponbare和“光身”突变体控制表皮毛性状的差异受单个主基因控制且“光身”为隐性,将该基因暂时命名为GLR3。利用该F₂群体,采用BSA法将GLR3定位在第6染色体上,进一步对F₂群体中417个典型的叶片光滑单株进行分子标记分析,将该基因定位在InDel标记ID27101和ID27199之间,与两标记相距皆为0.1 cM,两标记的物理位置相距98 kb。

关键词: 水稻, 光身, 遗传分析, 基因定位

Abstract: We obtained a glabrous leaf and hull mutant from a population of radiation mutagenesis of an indica rice cultivar R401. The mutant produced smooth leaves and hairless glumes under normal growth conditions. An F₂ population was developed from a cross between a japonica cultivar Nipponbare and the glabrous leaf and hull mutant. By investigating the performance of the F₂ population, we found that the mutant phenotype was controlled by a single recessive gene, temporarily designated GLR3. Bulked segregant analysis (BSA) based on the F₂ mapping population revealed that GLR3 is located on chromosome 6. By analyzing 417 typical glabrous leaf F₂ plants using molecular markers, GLR3 was mapped to a 0.2 cM interval between InDel markers ID27101 and ID27199, and the physical distance between the two markers is 98 kb. Thus we have mapped the gene GLR3, and our work will provide basis for future mechanistic analysis of GLR3 function.

Key words: rice, glabrous leaf and hull, genetic analysis, gene mapping

宋海冰, 汪斌, 陈壬杰, 郑小雅, 于世波, 兰涛. 水稻“光身”突变体glr3的遗传分析及基因定位[J]. 遗传, 2016, 38(11): 1012-1019.

Haibing Song, Bin Wang, Renjie Chen, Xiaoya Zheng, Shibo Yu, Tao Lan. Genetic analysis and gene mapping of the glabrous leaf and hull mutant glr3 in rice (Oryza sativa L.)[J]. Hereditas(Beijing), 2016, 38(11): 1012-1019.

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水稻“光身”突变体glr3的遗传分析及基因定位

Genetic analysis and gene mapping of the glabrous leaf and hull mutant glr3 in rice (Oryza sativa L.)

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