水稻“三明”显性核不育基因的定位

doi:10.3724/SP.J.1005.2012.00615

遗传 ›› 2012, Vol. 34 ›› Issue (5): 615-620.doi: 10.3724/SP.J.1005.2012.00615

水稻“三明”显性核不育基因的定位

杨泽茂¹, 谢小芳², 黄显波³, 王丰青¹, 童治军¹, 段远霖², 兰涛², 吴为人^1,2

1. 浙江大学农业与生物技术学院, 杭州 310000 2. 福建农林大学作物科学学院, 福州 350002 3. 福建省三明市农业科学研究所, 福建沙县365509

收稿日期:2012-01-08 修回日期:2012-03-26 出版日期:2012-05-20 发布日期:2012-05-25
通讯作者: 吴为人 E-mail:wrwu2005@yahoo.com.cn

Mapping of Sanming dominant genic male sterility gene in rice

YANG Ze-Mao¹, XIE Xiao-Fang², HUANG Xian-Bo³, WANG Feng-Qing¹, TONG Zhi-Jun¹, DUAN Yuan-Ling², LAN Tao², WU Wei-Ren^1,2

1. College of Agriculture & Biotechnology, Zhejiang University, Hangzhou 310000, China 2. College of Crop Science, Fujian Agriculture and Forestry University, Fuzhou 350002, China 3. SanMing Institute of Agricultural Science, Shaxian 365509, China

Received:2012-01-08 Revised:2012-03-26 Online:2012-05-20 Published:2012-05-25

摘要/Abstract

摘要： “三明显性核不育水稻”突变体是由福建省三明市农业科学研究所于2001年在杂交组合“SE21S/Basmati370”的F₂代群体中发现的。其不育性受1个显性基因控制(将该基因命名为SMS)。经过多代回交, 该显性不育基因已导入籼稻品种佳福占的遗传背景中(将该不育材料称为佳不育)。为了定位SMS, 文章将佳不育与粳稻品种日本晴杂交, 并将F₁与佳福占测交, 构建了一个作图群体。利用SSR和INDEL标记, 通过混合分离分析和连锁分析, 将SMS定位于第8号染色体上两个INDEL标记ZM30和ZM9之间, 约99 kb的区间内。该结果为克隆SMS奠定了基础。

关键词: 水稻, 显性雄性核不育, 基因定位

Abstract: The mutant of “Sanming Dominant Genic Male Sterile Rice” was found from an F₂ population of cross “SE2lS/Basmati370” by Sanming Institute of Agricultural Science in 2001. It has proven that the male sterility of this mutant is controlled by a dominant gene (named as SMS). By multiple backcrosses, this dominant male sterile allele was introduced into the genetic background of an indica rice cultivar Jiafuzhan (which was known as Jiabuyu). In order to map SMS, a mapping population was constructed by crossing Jiabuyu with a japonica cultivar Nipponbare and further crossing the F₁ with Jiafuzhan. By bulked segregant analysis and linkage analysis using SSR and INDEL markers, SMS was mapped to a 99 kb interval between INDEL markers ZM30 and ZM9 on chromosome 8. This result will facilitate cloning of SMS.

Key words: rice, dominant genic male sterility, gene mapping

杨泽茂，谢小芳，黄显波，王丰青，童治军，段远霖，兰涛，吴为人. 水稻“三明”显性核不育基因的定位[J]. 遗传, 2012, 34(5): 615-620.

YANG Ze-Mao, XIE Xiao-Fang, HUANG Xian-Bo, WANG Feng-Qing, TONG Zhi-Jun, DUAN Yuan-Ling, LAN Tao, WU Wei-Ren. Mapping of Sanming dominant genic male sterility gene in rice[J]. HEREDITAS, 2012, 34(5): 615-620.

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水稻“三明”显性核不育基因的定位

Mapping of Sanming dominant genic male sterility gene in rice

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