遗传 ›› 2008, Vol. 30 ›› Issue (8): 1033-1038.doi: 10.3724/SP.J.1005.2008.01033

• 研究报告 • 上一篇    下一篇

PRKAB2基因PCR-SSCP分析及其与屠宰性状和肉质性状的相关性

王金雷1; 朱庆1; 刘益平1; 杜华锐2   

  1. 1.四川农业大学动物科技学院, 雅安 625014;
    2.大恒家禽育种有限公司, 成都 610066

  • 收稿日期:2007-11-19 修回日期:2008-03-23 出版日期:2008-08-10 发布日期:2008-08-10
  • 通讯作者: 王金雷

Associations between SNP of chicken PRKAB2 gene and slaughter and meat quality traits

WANG Jin-Lei1; ZHU Qing1; LIU Yi-Ping1; DU Hua-Rui2   

  1. 1.College of Animal Science, Sichuan Agricultural University, Yaan 625014, China;
    2. Sichuan Daheng Poultry Breeding Company, Chengdu 610066, China
  • Received:2007-11-19 Revised:2008-03-23 Online:2008-08-10 Published:2008-08-10
  • Contact: WANG Jin-Lei

摘要: 采用测序和单链构象多态(Single-strand conformation polymorphism, SSCP)的方法, 分析了PRKAB2 (Protein kinase, AMP-activated, beta 2 non-catalytic subunit)基因在5个优质肉鸡纯系和3个杂交配套系中的遗传分布、遗传变异及群体杂合性等群体遗传信息, 并分析了PRKAB2基因对屠宰性状及肉质性状的遗传效应。结果发现该基因的CDS区域非常保守, 仅在外显子1的406 bp处发生点突变, 由T→C。野生型A基因可显著提高活重、屠体重、腿肌重和腹脂重(P < 0.05), 同时也会提高肉的嫩度。

关键词: 肉质性状, 屠宰性状, 鸡, PRKAB2基因

Abstract: SSCP (Single-strand conformation polymorphism, SSCP) technique was applied in this study to analyze the population genetic information about genetic distribution, variation and heterozygosity of PRKAB2(Protein kinase, AMP-activated, beta 2 non-catalytic subunit) gene in five purelines and three crossbreds. The genetic effects of PRKAB2 gene on fiber density and partial carcass traits were also analyzed. Only one point mutation T→C at base position 406 located among the first exon of the whole CDS was found in the whole CDS.The wild-type allele A had significant positive effects on live-weight, carcass-weight, leg-muscle-weight and abdomen-fat-weight (P<0.05), and had positive effects on meat tenderness.