人精母细胞重组频率和年龄相关性的分析

doi:10.3724/SP.J.1005.2011.00725

遗传 ›› 2011, Vol. 33 ›› Issue (7): 725-730.doi: 10.3724/SP.J.1005.2011.00725

人精母细胞重组频率和年龄相关性的分析

段涛¹, 杨庆岭², 王刘², 史庆华², 于德新¹

1. 安徽医科大学第一附属医院泌尿外科, 合肥 230022 2. 中国科学技术大学生命科学学院分子细胞遗传学实验室, 合肥微尺度物质科学国家实验室, 合肥 230027

收稿日期:2011-03-16 修回日期:2011-04-09 出版日期:2011-07-20 发布日期:2011-07-25
通讯作者: 于德新 E-mail:urology_2010@hotmail.com
基金资助:
国家重点基础研究发展规划(973计划)项目(编号：2006CB504003), 中国科学院知识创新工程重要方向性项目(编号: KSCX1- YW-R-51)和国家重大科学研究计划项目(编号：2007CB947401)资助

Correlation analysis between meiotic recombination frequencies and age in human spermatocyte

DUAN Tao¹, YANG Qing-Ling², WANG Liu², SHI Qing-Hua², YU De-Xin¹

1. Department of Urology, First Affiliated Hospital, Anhui Medical University, Hefei 230022, China 2. Hefei National Laboratory for Physical Sciences at Microscale, School of Life Science, University of Science Technology of China, Hefei 230027, China

Received:2011-03-16 Revised:2011-04-09 Online:2011-07-20 Published:2011-07-25

摘要/Abstract

摘要： 减数分裂遗传重组对同源染色体的正确分离和单倍体的正确形成起至关重要的作用, 但人们对人精母细胞减数分裂遗传重组机制了解的还很少。通过免疫荧光染色技术标记减数分裂I联会复合体上的MLH1(DNA错配修复蛋白)位点可以检测人精母细胞的重组。文章对10例可育男性进行分析, 发现每个细胞中重组位点数平均为 49.4士4.4, 范围为33~63, 具有显著的个体差异, 只有0.4％(1/220)的常染色体SC上缺少MLH1位点。进一步通过Spearman相关性分析, 分析了年龄因素与个体间重组位点差异的相关性, 结果提示年龄因素对常染色体及性染色体的重组均无影响。

关键词: 联会复合体, 遗传重组, 精母细胞, 年龄, 减数分裂

Abstract: Faithful meiotic recombination is essential for the segregation of homologous chromosomes and the formation of normal haploid gametes. Little is known about the mechanism of meiotic recombination in human germ cells. MLH1 (a DNA mismatch repair protein) foci on synaptonemal complexes (SCs) at prophase I of meiosis can be used to examine re-combination frequency. In 10 fertile men, the mean number of MLH1 foci per cell in all donors was 49.4 with a range from 33 to 63. There was significant variation in the recombination frequency found among 10 normal individuals: the mean frequencies of chromosomal recombination foci ranged from 47 to 52.7. The bivalents without recombination focus were rare, with a frequency of only 0.4%. Thus, achiasmate chromosomes appeared to be rare in human male meiosis. Spearman correlation analysis between age and the frequencies of recombination foci failed to get any significantly statistical correla-tion, suggesting that aging contributes nothing to the variation among individuals.

Key words: meiosis, synaptonemal complex, genetic recombination, spermatocyte, age

段涛，杨庆岭，王刘，史庆华，于德新. 人精母细胞重组频率和年龄相关性的分析[J]. 遗传, 2011, 33(7): 725-730.

DUAN Chao, YANG Qiang-Ling, WANG Liu, SHI Qiang-Hua, XU De-Xin. Correlation analysis between meiotic recombination frequencies and age in human spermatocyte[J]. HEREDITAS, 2011, 33(7): 725-730.

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人精母细胞重组频率和年龄相关性的分析

Correlation analysis between meiotic recombination frequencies and age in human spermatocyte

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