遗传 ›› 2011, Vol. 33 ›› Issue (1): 9-16.doi: 10.3724/SP.J.1005.2011.00009

• 综述 • 上一篇    下一篇

小RNA分子与精子发生调控

孟雅楠1,3, 孟丽军2, 宋亚娟1,3, 刘美玲3, 张秀军1,3   

  1. 1. 华北煤炭医学院生物科学系, 唐山 063000; 
    2. 唐山学院环境与化学工程系, 唐山 063000; 
    3. 国家人口计生委科学技术研究所生殖内分泌室, 北京 100081
  • 收稿日期:2010-03-31 修回日期:2010-10-12 出版日期:2011-01-20 发布日期:2011-01-20
  • 通讯作者: 张秀军 E-mail:zhangxiujun66@yahoo.com.cn
  • 基金资助:

    国国家自然科学基金项目(编号: 30671092)和国家人口计生委科学技术研究所中央级公益性科研院所基本科研业务费专项(编号: 2009GJSSJKB03)资助。

Small RNA molecules and regulation of spermatogenesis

MENG Ya-Nan1,3, MENG Li-Jun2, SONG Ya-Juan1,3, LIU Mei-Ling3, ZHANG Xiu-Jun1,3   

  1. 1. Department of Biology, Northchina Coal Medical College, Tangshan 063000, China;
     2. Department of Environment & Chemical Engineering, Tangshan College, Tangshan 063000, China
    3. Department of Reproductive Endocrinology, National Research Institute for Family Planning, Beijing 100081, China
  • Received:2010-03-31 Revised:2010-10-12 Online:2011-01-20 Published:2011-01-20
  • Contact: ZHANG Xiu-Jun1 E-mail:zhangxiujun66@yahoo.com.cn

摘要: 近来研究发现小RNA(small RNAs)可作为转录后及翻译水平上基因表达调节的重要调节因子, 利用小RNA来阐明调节精子发生的分子机制取得了显著进展。这些小RNA主要分为3类, 即小干扰RNA(siRNA)、微小RNA(miRNA)以及与piwi蛋白相互作用的RNA(piRNA)。在减数分裂和精子发生过程中, 小RNA具有多种生物学功能, 如利用siRNA体外转染或体内注射来敲低特定基因从而研究该基因在精子发生过程中的作用; miRNA可能参与精子发生中有丝、减数及后减数分裂阶段的基因表达调节; piRNA主要参与调节雄性生殖细胞减数及后减数分裂的过程, 在精子发生中起抑制反转录转座子(retrotransposons)的作用。文章对小RNAs合成、作用机制、功能及展望等最新进展进行了综述。

关键词: 精子发生, 小RNAs, 减数分裂, 基因表达调控

Abstract: Small RNA molecules (small RNAs) have recently emerged as important regulators of gene expression at the post-transcriptional or translation level. Significant progress has recently been made in utilizing small RNAs to elucidate the molecular mechanisms regulating spermatogenesis. There are three major small RNAs: small interfering RNAs (siRNAs), microRNAs (miRNAs), and piwi-interacting RNAs (piRNAs). Small-RNAs have diverse biological functions in meiosis and spermatogenesis, In vitro or in vivo, use of siRNA to knockdown genes is a way to study the function of genes of intrest in spermatogenesis. miRNA can be involved in the regulation of mitosis, meiosis, and postmeiosis in spermatogenesis. piRNAs are mainly involved in regulating the process of meiosis and postmeiosis, and repressing retrotransposon transposition in male germline cells. In this paper, we reviewed recent works on the synthesis, mechanism, function, and outlook of smallRNAs.

Key words: spermatogenesis, small RNAs, meiosis, regulation of gene expression