遗传 ›› 2001, Vol. 23 ›› Issue (2): 135-208.

• 论文 • 上一篇    下一篇

用天花粉蛋白基因转化小麦获得转基因植株

徐琼芳1;李连城1;马有志1;叶兴国1;李晓兵2;杜丽璞1;辛志勇1;张增艳1;邵鹏柱3 XU Qiong-fang1;LI Lian-cheng1;MA You-zhi1;YE Xing-guo1;LI Xiao-bing2;DU Li-pu1;XIN Zhi-yong1;ZHANG Zeng-yan1;SHAO Pang-zhu3   

  1. 1.中国农业科学院作物育种栽培研究所,农业部作物遗传育种重点实验室北京,100081; 2.中国科学院遗传研究所北京,100101; 3.香港中文大学生物系 1.Institute of Crop Breeding and Cultivation,CAAS,Beijing 100081,China; 2.Institute of Genetics, Academia Sinica,Beijing 100101,China; 3.Department of Biochemistry,The Chinese University of Hong Kong,Hong Kang,China
  • 收稿日期:1900-01-01 出版日期:2001-04-10 发布日期:2001-04-10

Wheat Transformation with Trichosantin Gene by Biolistic Particle

  • Received:1900-01-01 Online:2001-04-10 Published:2001-04-10

摘要: 取普通小麦品种京411未成熟胚诱导愈伤组织,10天左右,对820个胚性愈伤组织用含有35S启动子的天花粉蛋白(trichosanthin,TCS)基因轰击。2周后,将这些被轰击的愈伤组织转到含潮霉素50mg/L的筛选培养基上,经分化和生根,获得了33棵再生植株,经接饲毒蚜虫抗病性鉴定和PCR、Southern杂交分析,从中筛选出4株含有编码TCS的转基因小麦植株。转化频率为0.49%。
Abstract:Wheat immature embryo calli of 10 days culture from Jing 411,a common wheat variety, Triticum aestivum, were used as the receptor of Trichosanthin gene droven by 35S promotor to be bombardmented.Two weeks after bombardment, 820 embryonic calli were transferred onto selection medium containing 50mg/L hygromycin,and 33 plantlets with healthy roots were regenerated finaly from the calli. By the barley yellow dwarf virus resistance test of inoculating the wheat aphids carrying the virus on the plants, PCR analysis, and Southern blotting analysis, 4 plants were identified to be the transgenic wheats, containing the alien DNA that ecodes TCS gene. The transformed efficiency was around 0.12 percent.

关键词: transgenic wheat, 转化, 基因枪 Keywords, TCS基因, 转基因小麦