遗传 ›› 2008, Vol. 30 ›› Issue (11): 1477-1486.doi: 10.3724/SP.J.1005.2008.01477

• 研究报告 • 上一篇    下一篇

玉米株高和穗位高的QTL定位

杨晓军1, 2;路明1, 3;张世煌1;周芳1;曲延英2;谢传晓1   

  1. 1. 中国农业科学院作物科学研究所, 国家农作物基因资源与遗传改良重大科学工程, 北京 100081;
    2. 新疆农业大学农学院, 乌鲁木齐 830052;
    3. 吉林省农业科学院玉米研究所, 长春 136100
  • 收稿日期:2008-02-27 修回日期:2008-05-10 出版日期:2008-11-10 发布日期:2008-11-10
  • 通讯作者: 谢传晓;杨晓军

QTL mapping of plant height and ear position in maize (Zea mays L.)

YANG Xiao-Jun1, 2;LU Ming1, 3;ZHANG Shi-Huang1;ZHOU Fang1;QU Yan-Ying2;XIE Chuan-Xiao1   

  1. 1. National Key Facilities for Crop Gene Resource and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China;
    2. Agronomy College, Xingjiang Agricultural University, Urumqi 830052, China;
    3. Institute of Maize Research, Jilin Academy of Agricultural Sciences, Changchun 136100, China
  • Received:2008-02-27 Revised:2008-05-10 Online:2008-11-10 Published:2008-11-10
  • Contact: XIE Chuan-Xiao;YANG Xiao-Jun

摘要: 摘要: 用玉米自交系掖478和丹340构建了397个F2:3家系群体, 利用双亲间多态的150个共显性SSR标记绘制分子连锁图谱, 图谱总长度1 478.7 cM, 标记间平均距离10.0 cM。在5种环境下对株高和穗位高性状进行鉴定, 复合区间作图法检测到21个株高QTL和25个穗位高QTL。于第1和5染色体的umc2025-umc1035及umc1822-bnlg1118区域检测到平均贡献率分别为12.2%和14.9%的株高QTL。于第3和5染色体的phi029-umc1102及phi109188-bnlg1118区域检测到平均贡献率达到10.2%和22.8%的穗位高QTL。第5染色体的Bin5.05-5.07区域可能存在控制株高和穗位高的主效QTL。株高和穗位高的基因作用方式主要是加性和部分显性效应。文章还分析了群体大小及试验环境对株高和穗位高QTL定位结果的影响

关键词: QTL定位, 穗位高, 株高, 玉米

Abstract: Abstract: A molecular linkage map for the maize hybrid of cross between Ye478 and Dan340 was constructed by using 150 co-dominant SSR markers. The total map length was 1 478.7 cM with an average interval of 10.0 cM. Composite interval mapping was used to identify the plant height (PH) and ear position (EH) QTL at 5 environments based on the phenotypic data of 397 F2:3 families. Then 21 PH and 25 EH relevant QTL were identified. The mean contribution of 12.2% and 14.9% QTL for plant height was identified at the interval of umc2025 - umc1035 on chromosome 1 and umc1822 - bnlg1118 on chromosome 5, respectively. Meanwhile, the mean contribution of 10.2% and 22.8% to ear position were identified at the interval of phi029 - umc1102 on chromosome 3 and phi109188 - bnlg1118 on chromosome 5. The main QTL for PH and EH were both found at the regions of Bin5.05 - 5.07 on chromosome5. The additive and partial dominant effects were the main genetic basis for plant height and ear position in maize. The effect of population size and environments on QTL map-ping were analyzed.