遗传 ›› 2012, Vol. 34 ›› Issue (7): 857-862.doi: 10.3724/SP.J.1005.2012.00857

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分枝杆菌中基因敲除操作工具研究进展

杜庆林, 樊祥宇, 毛金校, 谢建平   

  1. 西南大学生命科学学院现代生物医药研究所, 三峡库区生态环境与生物资源省部共建国家重点实验室培育基地, 重庆北碚, 400715
  • 收稿日期:2011-12-07 修回日期:2012-04-04 出版日期:2012-07-20 发布日期:2012-07-25
  • 通讯作者: 谢建平 E-mail:georgex@swu.edu.cn
  • 基金资助:

    国家重要传染病科技重大专项(编号:2008ZX10003-006, 2012ZX10003003-004), 国家自然科学基金项目(编号:81071316), 教育部新世纪优秀人才支持计划(编号:NCET-2011), 中央高校基本科研业务费专项资金(编号:XDJK2009A003, XDJK2011D006)和西南大学研究生科技创新基金项目(编号:kb2010017)资助

Progression on genetic knockout tools in Mycobacterium

DU Qin-Lin, FAN Xiang-Yu, MAO Jin-Xiao, XIE-Jian-Ping   

  1. Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, School of Life Sciences, Southwest University, Chongqing 400715, China
  • Received:2011-12-07 Revised:2012-04-04 Online:2012-07-20 Published:2012-07-25

摘要: 致病性分枝杆菌仍然是人类健康的重要威胁。相对于传统模式原核生物, 在分枝杆菌中进行基因敲除一直是一个难题。文章介绍了分枝杆菌中用于基因敲除的转运载体和序列特异性重组系统的研究进展, 并进一步介绍了新近发展的分枝杆菌重组工程在基因敲除中的应用。最后, 对分枝杆菌基因敲除系统的发展历程进行了回顾, 并对未来的发展方向进行了展望。

关键词: 分枝杆菌, 基因敲除, 转运载体, 序列特异重组, 重组工程

Abstract: Pathogenic mycobacteria were and remain a heavy burden to public health. Unfortunately, genetic manipulation including knockout technologies of Mycobacterium is difficult compared with other traditional model organisms. To overcome this obstacle, achievements in Mycobacterium knockout technologies were summarized, including deliv-ery vector, sequence-specific recombination system, as well as the recently developed recombinogenic engineering and its application. The future for this tool innovation is also addressed.

Key words: Mycobacterium, knockout, delivery vector, sequence-specific recombinase, recombinogenic engineering